نوع مقاله : مقاله پژوهشی

نویسندگان

1 مرکز آموزش عالی شهید باکری میاندوآب، دانشگاه ارومیه، ارومیه، ایران

2 فارغ التحصیل مهندسی گیاهان دارویی، مرکز آموزش عالی شهید باکری میاندوآب، دانشگاه ارومیه، ارومیه، ایران

چکیده

نعنا سبز، Mentha spicata L. و پونه کوهی، Mentha longifolia Lکاربرد‌های فراوانی در صنایع غذایی و طب سنتی ایران دارند. سرشاخه­های گیاهان جمع‌آوری شده و اسانس آن‌ها به روش تقطیر با آب (کلونجر) استخراج گردید. عصاره‌گیری با استفاده از دو روش ماسراسیون (حلال­های آبی، هیدروالکلی و اتانولی) و روش سوکسله (حلال اتانولی) انجام شد. محتوای ترکیبات فنولی کل، محتوای فلاوونوئید کل، ظرفیت پاداکسایشی و درصد مهار تولید رادیکال آزاد اندازه­گیری شد. نتایج نشان داد محتوای ترکیبات فنولی، فلاوونوئیدی، ظرفیت پاداکسایشی و درصد بازدارندگی رادیکال آزاد DPPHتحت تأثیر روش استخراج قرار گرفت. بیش‌ترین میزان ترکیبات فنولی و فلاوونوئید کل در گیاه نعنا سبز در روش سوکسله به‌دست آمد. بالاترین ظرفیت پاداکسایشی مربوط به عصاره هیدروالکلی نعنا سبز بود. روش استخراج با سوکسله بدلیل هزینه فرآوری پایین آن، سادگی عمل، صرف زمان کمتر و حلالیت بیشتر در مقایسه با دیگر روش‌های سنتی مانند خیساندن و پرکولاسیون برای استخراج ترکیبات فنولی توصیه می‌شود. استفاده از سیستم دو حلالی در روش خیساندن با حلال‌های دیگر برای دست‌یابی به خاصیت پاداکسایشی بالا نیازمند تحقیقات بیشتر است.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of phenolic, flavonoid compounds and antioxidative properties of Mentha spicata L. and Mentha longifolia L. 's extracts by different extraction methods

نویسندگان [English]

  • Nayer Mohammadkhani 1
  • Jila Abbaszadeh 2

1 Shahid Bakeri High Education Center if Miandoab, Urmia University, Urmia, Iran

2 Graduated of Medicinal Plants Engineering, Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran

چکیده [English]

Spearmint (Mentha spicata L.) and Horsemint (Mentha longifolia L.) have abundant use in food expenses and Iranian Traditional Medicine. Tha aerial parts of both plants were collected and essential oil was isolated by hydrodistillation method (Clevenger). The extraction was conducted by maceration method (aqueous, hydroalcoholic and ethanolic solvents) and soxhlet method (ethanolic solvent). Total phenolic compounds content, Total flavonoid content, antioxidant capacity and free radical inhibition were measured. The results showed that total phenolic compounds and flavonoid content, total antioxidant capacity and percentage of free radical inhibition in applied extraction methods was different (p < 0.05). The highest total phenolic compounds and flavonoid was obtained by soxhlet method in spearmint. The highest total antioxidant capacity were belong to hydroalcoholic extract in spearmint. Soxhlet extraction method is recommended because of its low processing cost, simplicity of operation, consume lesser time and solvent compared to other traditional methods such as soaking or percolation to extract phenolic compounds using other solvents. The use of a two-solvent system in the soaking process with other solvents requires further research to achieve high antioxidant properties.

کلیدواژه‌ها [English]

  • extraction methods
  • phenol
  • flavonoid
  • total antioxidant capacity
  • inhibition of free radical
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