جداسازی باکتری های مولد بیودمولسیفایر از رسوبات آلوده به نفت پالایشگاه نفت آبادان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استاد مرکز تحقیقات بیوتکنولوژی، دانشگاه شهید چمران اهواز ، اهواز، ایران.

2 کارشناسی ارشد گروه زیست شناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

 



دمولسیفایرهای شیمیایی که برای شکست امولسیون‌های آب در نفت بکار می‌روند سمی هستند و اثرات زیان‌باری بر محیط-زیست دارند. به همین دلیل بیودمولسیفایرها به عنوان ترکیبات سازگار با محیط زیست به عنوان یک جایگزین مطرح هستند. هدف از تحقیق حاضر دستیابی به باکتری‌های مولد بیودمولسیفایر است. برای این منظور با نمونه‌گیری از رسوبات نفتی، جدایه‌های مولد بیـودمولسیفایر با استفاده از روش‌های غنی‌سازی در محیط پایه نمکی با پارافین مایع جداسازی و جدایه‌های برتر با استفاده از روش‌های کمی و کیفی غربالگری انتخاب شدند. شرایط رشد بهینه این جدایه‌ها و اثر نوع منبع کربن بر تولید بیودمولسیفایر بررسی و با استفاده از روش‌های فنوتیپی و تعیین توالی ژن 16S rRNA تعیین هویت گردیدند. در نتیجه‌ی این تحقیق جدایه‌هایHS20 و HS22 به ترتیب با 43/71 و 71/85 درصد شکست امولسیون آب در نفت سفید به عنوان Alcaligenes sp. strain HS20 و Alcaligenes sp. strain HS22 شناسایی شدند. دما، pH و منبع نیتروژن بهینه رشد جدایهHS20 40 درجه سانتی‌گراد، 7 و سدیم نیترات و برای جدایه HS22 35 درجه سانتی-گراد، 7 و آمونیوم نیترات بود. بیودمولسیفایر جدایه HS20 خارج سلولی و جدایه HS22 در دو موقعیت خارج سلول و متصل به سلول حضور دارد. هر دو جدایه در حضور منابع کربنی هیدروفیل یا مخلوط هیدروکربن‌های هیدروفیل و هیدروفوب توانایی تولید بیودمولسیفایر دارند. نتایج این پژوهش نشان داد این جدایه‌ها توانایی تولید بیودمولسیفایر و شکست امولسیون آب در نفت را در حد مناسبی دارند. بنابراین ارزیابی پتانسیل این سویه‌ها به مـنـظـور بهبود شکست امولسیون-های نامطلوب آب در نفت خام در مقیاس صنعتی پیشنهاد می‌گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Isolation of biodemulsifier producing bacteria from petroleum-contaminated deposits of Abadan Oil Refinery

نویسندگان [English]

  • Hossein motamedi 1
  • Hoda Sabati 2

1 Professor Biotechnology Research Center, Shahid Chamran University of Ahvaz Ahvaz, Iran.

2 M cs Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

چکیده [English]

Chemical demulsifiers that are used for breaking water in oil emulsions, are toxic and have adverse side effects for environment. So, biodemulsifiers have been suggested as an environmental friendly alternative. The aim of this study was to achieve biodemulsifier producing bacteria. For this purpose, biodemulsifier producing bacteria were isolated from deposits in oil reservoirs in petroleum refinery through enrichment in saline-based enrichment medium with liquid paraffin and screened in quantitative and qualitative screening methods. Optimum growth condition of the selected isolates as well as the effect of carbon source on biodemulsifier production were assessed and these isolates were identified based on phenotypic methods a sequencing of 16S rRNA gene. As a result, HS20 and HS22 isolates with 71.43 and 85.71percentage demulsification of water in kerosene were identified as Alcaligenes sp. strain HS20 and Alcaligenes sp. strain HS22, respectively. Optimum temperature, pH and nitrogen source for HS20 were as 40°C, 7 and NaNO3 and for HS22 were 35°C, 7 and NH4NO3. The HS20 biodemulsifier was extracellular while in case of HS22 was in both extracellular and cell attached forms. Both isolates were able to produce biodemulsifier in the presence of hydrophilic and/or mixture of hydrophilic and hydrophobic hydrocarbons. The results of this study showed that these isolates have appropriate biodemulsifier production and emulsion breaking. Therefore, evaluation of their potential to improve the breakdown of undesirable water in crude oil emulsions at industrial scale is proposed.

کلیدواژه‌ها [English]

  • Biodemulsifier
  • Biological separation
  • Water in oil emulsion
  • Emulsion breaking
 

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