نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم باغبانی و فضای سبز، مجتمع آموزش عالی میناب، دانشگاه هرمزگان، هرمزگان، ایران.

2 دانشیار، گروه علوم باغبانی و فضای سبز، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.

10.22051/jab.2023.42473.1536

چکیده

‌گل مریم (.Polianthes tuberosa L) یکی از گیاهان سوخوار زینتی است و از گل‌های بریده مهم در مناطق گرمسیری و نیمه‌گرمسیری است. ویتامین‌ها در مقادیر کم نیز برای رشد و نمو عادی بافت‌ها در گیاه ضرورت دارند. وجود این دسته از مواد برای رشد گیاه در محیط‌های کشت بافت و برخی از آن‌ها در گیاهان گلخانه‌ای ثابت شده است. بدین منظور در پژوهش حاضر، تاثیر ویتامین‌های A، B، C و E بر صفات مورفولوژیکی، فیزیولوژیکی و بیوشیمیایی گل مریم مورد بررسی قرار گرفت. ویتامین‌های به‌کار گرفته شده در این پژوهش شامل ویتامین B (تیامین)،C (اسید اسکوربیک)، E (آلفا توکوفرول) و A (رتینول) بودند که در سه سطح 50، 100 و 150 میلی‌گرم در لیتر و طی سه مرحله (30، 40 و 50 روز پس از کاشت) و به صورت محلول‌پاشی روی گیاهان اعمال شدند. نتایج مقایسه میانگین داده ها نشان داد که بیشترین طول گل آذین، قطر گلچه و وزن تر اندام هوایی مربوط به تیمار اسید آسکوربیک (100 میلی‌گرم در لیتر) بود و بیشترین میزان طول ریشه سوخ و اسانس در غلظت 50 میلی‌گرم در لیتر اسید آسکوربیک و بیشترین میزان پروتئین برگ و عمر گلجایی در غلظت 150 میلی‌گرم در لیتر اسید آسکوربیک حاصل شد. همچنین بیشترین تعداد گلچه و قند احیا مربوط به غلظت 100 میلی‌گرم در لیتر تیامین و بیشترین میزان فعالیت آنزیم آسکوربات پراکسیداز مربوط به غلظت 150 میلی‌گرم در لیتر تیامین بود. بیشترین میزان فعالیت آنزیم پر‌اکسیداز و کلروفیل a به ترتیب مربوط به غلظت‌های 100 و 50 میلی‌گرم در لیترآلفا توکوفرول بود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The response of Polianthes tuberosa L. to the use of some vitamins

نویسندگان [English]

  • Mehrdad Babarabie 1
  • زارعی Zarei 2

1 Assistant Porfessor, Department Green Space Science and Engineering, Minab Higher Education Center, Hormozgan University, Hormozgan, Iran.

2 Associate Professor, Department of Green Space Science and Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

چکیده [English]

Polianthes tuberosa L. is one of the ornamental plants and is one of the important cut flowers in tropical and semi-tropical regions. Vitamins in small amounts are necessary for the normal growth and development of tissues in the plant. The existence of this group of materials for plant growth has been proven in tissue culture and some of them in greenhouse plants. For this purpose, in the present study, the effect of vitamins A, B, C and E on the morphological, physiological and biochemical traits of tuberosa flower was investigated. The vitamins used in this study included vitamin B,, C ,, E (alpha tocopherol) and A at three levels of 50, 100 and 150 mg/liter and during three stages ( 30, 40 and 50 days after planting) and were applied to the plants as a foliar spray. The results of comparing the average data showed that the highest length of inflorescence, diameter of flower and wet weight of shoot organ were related to the treatment of ascorbic acid (100 mg/liter). The highest amount of root length of bulbs and essential oil was obtained in the concentration of 50 mg/liter of ascorbic acid, and the highest amount of leaf protein and life expectancy was obtained in the concentration of 150 mg/liter of ascorbic acid. Also, the highest number of florets and reducing sugars were related to the concentration of 100 mg/liter of thiamine and the highest activity of ascorbate peroxidase enzyme was related to the concentration of 150 mg/liter of thiamine.

کلیدواژه‌ها [English]

  • Ascorbic acid
  • Growth
  • Polianthes tuberosa L
  • Stem length
  • Vitamins
[1]     Shagufta Naz. (2012). In vitro propagation of tuberose (Polianthes tuberosa). Journal of medicinal plants research, 6(24), 4107–4112. DOI: 10.5897/jmpr12.647
[2]     Jowkar, M. M., & Salehi, H. (2006). The Effects of different preservative solutions on the Vase life of Cut tuberose (Polianthes tuberose L.) cv. Goldorosht-e-Mahallat. JWSS-isfahan university of technology, 10(3), 299–309.
[3]     Antonopoulou, C., Dimassi, K., Therios, I., Chatzissavvidis, C., & Tsirakoglou, V. (2005). Inhibitory effects of riboflavin (Vitamin B2) on the in vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (Prunus amygdalus$\times$ P. persica). Scientia horticulturae, 106(2), 268–272.
[4]     Abdel-Halim, S. M. (1995). Effect of some vitamins as growth regulators on growth, yield and endogenous hormones of tomato plants during winter. Egyptian journal of applied sciences, 10(12), 322–334. https://ejoh.journals.ekb.eg/article_1326_9ee9187d5bc143e431397588af234a4a.pdf
[5]     K.M, G. E.-D. (2005). Physiological studies on the effect of some vitamins on growth and oil content in sunflower plant. Egyption journal of basic and applied science, 20, 560–571.
[6]     Youssef, A. A., & Talaat, I. M. (2003). Physiological response of rosemary plants to some vitamins. Egyptian pharmaceutical journal [national research center], 1(1), 81–93.
[7]     Nahed, G. A. A., Lobna, S. T., & Soad, M. M. I. (2009). Some studies on the effect of putrescine , ascorbic acid and thiamine on growth , flowering and some chemical constituents of gladiolus plants at nubaria. Ozean journal of applied sciences, 2(2), 169–179.
[8]     El-Aziz, N. G. A., El-Quesni, F. E. M., & Farahat, M. M. (2007). Response of vegetative growth and some chemical constituents of Syngonium podophyllum L. to foliar application of thiamine, ascorbic acid and kinetin at Nubaria.World journal of agricultural sciences, 3(3), 301–305. https://www.cabidigitallibrary.org/doi/full/10.5555/20073155892
[9]     El-Fawakhry, F. M., & El-Tayeb, H. F. (2003). Effect of some amino acids and vitamins on chrysanthemum production. Journal agriculture research alexandria university, 8(4), 755–766.
[10]   Ieamtim, P., Buanong, M., & Kanlayanarat, S. (2008). Role of ascorbic acid on vase life of red ginger (alpinia purpurata (vieill.) k. schum) [presentation]. Acta horticulturae (pp. 287–290). DOI: 10.17660/actahortic.2008.804.39
[11]   Arango, Y., & Heise, K.-P. (1998). Localization of $α$-tocopherol synthesis in chromoplast envelope membranes of Capsicum annuum L. fruits. Journal of experimental botany, 49(324), 1259–1262.
[12]   Sheppard, A. J., Pennington, J. A. T., & Weihrauch, J. L. (1993). Analysis and distribution of vitamin E in vegetable oils and foods. In Vitamin e in health and disease (pp. 9–32). CRC Press.
[13]   Anwar, M., Sahito, H. A., Hassan, I., Abbasi, N. A., Ahmed, H. A., Bhatti, M. A., … Abro, A. (2014). Effect of pre harvest treatment of salicylic on growth and vase life of tuberose with aroma environment. Wudpecker journal of agricultural research, 3(2), 50–57.
[14]   Barnes, J. D., Balaguer, L., Manrique, E., Elvira, S., & Davison, A. W. (1992). A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environmental and experimental botany, 32(2), 85–100.
[15]   In-Byung, C., Motomura, S. K., Inamota, M. D., & Mori, G. (2007). Multivariate analysis of relation between preharvest environmental factors, postharvest morphological and physiological factors, and vase life of cut ‘Asami Red’roses. Journal of the japanese society for horticultural science, 76, 66–72.
[16]   Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and cell physiology, 22(5), 867–880. DOI: 10.1093/oxfordjournals.pcp.a076232
[17]   Somogyi, M., & others. (1952). Notes on sugar determination. Journal of biological chemistry, 195, 19–23.
[18]   Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1–2), 248–254.
[19]   Kheiry, A., Khalighi, A., Mostofi, Y., & Naderi, R. (2011). Effects of gibberellic acid (GA3) and benzyladenine on tuberose quality and quantity. Journal of crops improvement, 13(1), 9–20. (In Persian). https://jci.ut.ac.ir/article_23992_en.html
[20]   Evans, R. Y., & Reid, M. S. (1988). Changes in carbohydrates and osmotic potential. Journal of the american society for horticultural science, 113(6), 884–888. https://ucanr.edu/sites/Postharvest_Technology_Center_/files/228403.pdf
[21]   Smirnoff, N. (2005). Antioxidants and reactive oxygen species in plants. Wiley Online Library.
[22]   Conklin, P. L., & Barth, C. (2004). Ascorbic acid, a familiar small molecule intertwined in the response of plants to ozone, pathogens, and the onset of senescence. Plant, cell & environment, 27(8), 959–970.
[23]   Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., & Yoshimura, K. (2002). Regulation and function of ascorbate peroxidase isoenzymes. Journal of experimental botany, 53(372), 1305–1319.
[24]   El-Lethy, S. R., Ayad, H. S., & Reda, F. (2011). Effect of riboflavin, ascorbic acid and dry yeast on vegetative growth, essential oil pattern and antioxidant activity of geranium (Pelargonium graveolens L.). American-eurasian journal of agricultural & environmental sciences, 10(5), 781–786.
[25]   Rosales, M. A., Ruiz, J. M., Hernández, J., Soriano, T., Castilla, N., & Romero, L. (2006). Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation. Journal of the science of food and agriculture, 86(10), 1545–1551.
[26]   Bedour, A. A. L., & Rawia, A. E. (2011). Improving gladiolus growth, flower keeping quality by using some vitamins application. Journal of american science, 7(3), 169–174.
[27]   Mahgoub, M. H., Abd El Azis, N., & Mazhar, A. M. A. (2011). Response of Dahlia pinnata L. plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. Am eurasian j agric environ sci, 10(5), 769–775.
[28]   Hajreza, M. R., Hadavi, E., Zeynanlou, A. A., Mirzapour, M. H., & Naeini, M. R. (2013). Effect of different levels of citric acid and salicylic acid at pre-harvesting stage on vase-life of rose (Rosa hybrida L.) cut flower. Journal of science and technology of greenhouse culture-isfahan university of technology, 4(16), 99–109. (In Persian). https://www.cabidigitallibrary.org/doi/full/10.5555/20143039077
[29]   Ichimura, K., & Goto, R. (2002). Extension of vase life of cut Narcissus tazetta var. chinensis flowers by combined treatment with STS and gibberellin A3. Journal of the japanese society for horticultural science, 71(2), 226–230. DOI: 10.2503/jjshs.71.226
[30]   M.A, A., F, N., F, S., & S,  and A. (2001). Effect of some chemicals on keeping quality and vase life of (Gladiolus grandiflorus sect. Blandus cv. Aarti) by using leaves of allelopathic plants. African journal of biotechnology, 9(30), 4681–4686.
[31]   El-Kobisy, O.S., K.A. Kady, R. A. M. and R. A. A. (2005). Response of pea plant (Pisum sativum L.) to treatment with ascorbic acid. Egyptian journal of applied sciences, Zagazig University, 20(6A), 36–50.
[32]   Blokhina, O., Virolainen, E., & Fagerstedt, K. V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of botany, 91(2), 179–194.
[33]   Nahed, Aziz, G. A., Azza, Mazher, A. M., & Farahat, M. M. (2010). Response of vegetative growth and chemical constituents of thuja orientalis l. plant to foliar application of different amino acids at nubaria. Journal of american science, 6(3), 295–301.
[34]   Ezhilmathi, K., Singh, V. P., Arora, A., & Sairam, R. K. (2007). Effect of 5-sulfosalicylic acid on antioxidant activity in relation to vase life of Gladiolus cut flowers. Plant growth regulation, 51(2), 99–108. DOI: 10.1007/s10725-006-9142-2
[35]   Menesi, F. A., Nofal, E. M. S., & El-Mahrouk, E. M. (1991). Effect of some growth regulators on Calendula officinalis L. Egyptian journal of applied science, 6, 1–15.
[36]   Razem, F. A., El-Kereamy, A., Abrams, S. R., & Hill, R. D. (2006). The RNA-binding protein FCA is an abscisic acid receptor. Nature, 439(7074), 290–294.
[37]   Barth, C., De Tullio, M., & Conklin, P. L. (2006). The role of ascorbic acid in the control of flowering time and the onset of senescence. Journal of experimental botany, 57(8), 1657–1665.
[38]   Van Doorn, W. G., & D’hont, K. (1994). Interaction between the effects of bacteria and dry storage on the opening and water relations of cut rose flowers. Journal of applied microbiology, 77(6), 644–649.
[39]   Lerslerwong, L., Ketsa, S., & van Doorn, W. G. (2009). Protein degradation and peptidase activity during petal senescence in Dendrobium cv. Khao Sanan. Postharvest biology and technology, 52(1), 84–90.
[40]   Noctor, G., & Foyer, C. H. (1998). Ascorbate and Glutathione: Keeping Active Oxygen under Control. Annual review of plant biology, 49(1), 249–279. DOI: 10.1146/annurev.arplant.49.1.249
[41]   El-Quesni, F., El-Aziz, A., & Maga, M. (2009). Some studies on the effect of Ascorbic Acid and α-tocopherol on the growth and some chemical composition of Hibiscus rosa sinensis L. Ozean journal, 2(2), 159–167.
[42]   El-Aziz, N. G. A. (2007). Stimulatory effect of NPK fertilizer and benzyladenine on Aebi, H. Methods in enzymology, 121–126.
[43]   Agarwal, S., & Pandey, V. (2004). Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. Biologia plantarum, 48(4), 555–560. DOI: 10.1023/B:BIOP.0000047152.07878.e7
[44]   Huang, C., He, W., Guo, J., Chang, X., Su, P., & Zhang, L. (2005). Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant. Journal of experimental botany, 56(422), 3041–3049.
[45]   Blekas, G., Tsimidou, M., & Boskou, D. (1995). Contribution of $α$-tocopherol to olive oil stability. Food chemistry, 52(3), 289–294.
[46]   Frankel, E. N., Huang, S.-W., Kanner, J., & German, J. B. (1994). Interfacial phenomena in the evaluation of antioxidants: bulk oils vs emulsions. Journal of agricultural and food chemistry, 42(5), 1054–1059.
[47]   Martínez de la Cuesta, P. J., Rus Martínez, E., & Galdeano Chaparro, M. (1995). Enranciamiento oxidativo de aceites vegetales en presencia de α-tocoferol. Grasas y aceites, 46(6), 349–353. DOI: 10.3989/gya.1995.v46.i6.951
[48]   Dixit, V., Pandey, V., & Shyam, R. (2001). Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad). Journal of experimental botany, 52(358), 1101–1109.
[49]   Smirnoff, N., & Wheeler, G. L. (2000). Ascorbic acid in plants: biosynthesis and function. Critical reviews in plant sciences, 19(4), 267–290.
[50]   Chen, Z., & Gallie, D. R. (2004). The ascorbic acid redox state controls guard cell signaling and stomatal movement. The plant cell, 16(5), 1143–1162.
[51]   Dubey, V. S., Bhalla, R., & Luthra, R. (2003). An overview of the non-mevalonate pathway for terpenoid biosynthesis in plants. Journal of biosciences, 28, 637–646.
[52]   Hassanein, R. A. M. (2003). Effect of some amino acids, trace elements and irradiation on fennel (foeniculum vulgare l.) [Mster Thesis].
[53]   Abou Dahab, T. A. M., & El-Aziz, N. G. A. (2006). Physiological effect of diphenylamin and tryptophan on the growth and chemical constituents of Philodendron erubescens plants. World journal of agricultural sciences, 2(1), 75–81.
[54]   El-Wahed, M., Amin, A. A., & Rashad, E. M. (2006). Physiological effect of some bioregulators on vegetative growth, yield and chemical constituents of yellow maize plants. World journal of agricultural sciences, 2(2), 149–155. https://www.cabidigitallibrary.org/doi/full/10.5555/20073201725
[55]   El-Gabas, N. M. M. (2006). Physiological studies on the effect of ascorbic acid and micronutrients on sunflower plants grown under salinity stress’. B. Sc.(Botany). Faculty of Science, Al-Azhar University.
[56]   Salem, H. M., Abdel-Rahman, S., & Mohamed, S. I. (2000). Response of sugar beet plants to boron and ascorbic acid under filed conditions’. Journal faculty education, Ain Shams University, 48, 1–20.
[57]   Buchanan-Wollaston, V. (1997). The molecular biology of leaf senescence. Journal of experimental botany, 48(307), 181–199. DOI: 10.1093/jxb/48.2.181
[58]   Hossain, Z., Kalam Azad Mandal, A., Kumar Datta, S., & Krishna Biswas, A. (2006). Decline in ascorbate peroxidase activity - A prerequisite factor for tepal senescence in gladiolus. Journal of plant physiology, 163(2), 186–194. DOI: 10.1016/j.jplph.2005.03.004
[59]   Jin, J., Shan, N., Ma, N., Bai, J., & Gao, J. (2006). Regulation of ascorbate peroxidase at the transcript level is involved in tolerance to postharvest water deficit stress in the cut rose (Rosa hybrida L.) cv. Samantha. Postharvest biology and technology, 40(3), 236–243. DOI: 10.1016/j.postharvbio.2006.01.014
[60]   Sujata, A., Vijaai Singh, N., & and Sharma, T. . (2003). Effect of chemical preservative on enhancing vase life of Gerbera treatment with STS and Gibberelin A3. Journal of the japanese society for horticultural science, 71, 216–230.
[61]   Ravanbakhsh, A., Mobasser, H. R., & Hasandokht, M. R. (2017). Effect of ascorbic acid and acetyl salicylic acid on the quality and vase life of cut flowers Glasiolus (Gladiolus persicus). International journal of agriculture and biosciences, 6(1), 31–33. http://www.ijagbio.com/pdf-files/volume-6-no-1-2017/31-33.pdf