نوع مقاله : مقاله پژوهشی

نویسنده

دانشیار، گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران

چکیده

روش‌های مرسوم شناسایی بر اساس صفات ریخت‌شناسی هرچند در شناسایی گونه‌های مختلف آسپرژیلوس کارایی قابل قبولی دارند اما در تشخیص نژادها و جمعیت‌های گونه‌های این جنس عموماً ناکارآمد هستند، این موضوع در مورد شناسایی جمعیت‌های Aspergillus flavus که یکی از گونه‌های بسیار مهم این جنس در زمینه‌های اقتصادی، بهداشتی است نیز صدق می‌کند، در این تحقیق جمعیت‌های این گونه که از پنج محصول خشکباری کشور بدست آمده بودند بر اساس صفات میکروسکوپی و ماکروسکوپی مورد شناسایی قرار گرفتند، علاوه بر این، از بیست پرایمر استاندارد شده بر اساس روش
Inter Simple Sequence Repeats ISSR)) برای بررسی جمعیت‌های این گونه استفاده شد. نتایج آنالیزهای چند متغیره بر اساس یافته‌های ISSR نشان می‌دهد با استفاده از این روش می‌توان جمعیت‌های مختلف Aspergillus flavus جدا شده از میزبان‌های مختلف و مناطق مختلف را از یکدیگر تشخیص داد.

کلیدواژه‌ها

عنوان مقاله [English]

Application of ISSR method in systematic identification and segregation of populations of Aspergillus flavus

نویسنده [English]

  • Amir Abbas Minaeifar

Associate Professor, Department of Biology, Payam Noor University, Tehran, Iran

چکیده [English]

Conventional morphological identification methods, although reasonably effective in identifying different species of Aspergillus, are generally ineffective in identifying the races and populations of Aspergillus species; it is also true about Aspergillus flavus, which is one of the most important Aspergillus species in Economic and health. In this study, in addition to morphological identification based on microscopic and macroscopic traits of Aspergillus flavus isolates from five dried fruit products, Twenty standardized primers based on ISSR method were used to study the populations of this species. Statistical Multivariate analyzes based on ISSR findings, show different populations from different sources and different locations of Aspergillus flavus can be distinguish from each other with use of this method.

کلیدواژه‌ها [English]

  • Aspergillus flavus
  • ISSR
  • Nuts
  • Morphology
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