نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار،گروه علوم پایه،دانشکده پیرادامپزشکی،دانشگاه اردکان،اردکان،ایران

2 دانشجوی ارشد علوم جانوری گرایش سلولی- تکوینی،دانشکده پیرا دامپزشکی ، دانشگاه اردکان،اردکان ،ایران

10.22051/jab.2021.37075.1437

چکیده

دیابت از شایع ترین بیماری های قرن حاضر است. عوارض زیاد داروهای شیمیایی مورد استفاده در درمان آن گرایش به درمان را توسط گیاهان دارویی که دارای عوارض جانبی کمتری هستند روز به روز افزایش می دهد. فلاونوییدهای موجود درگیاهان با اثر آنتی اکسیدانی خود توانایی مهار استرس اکسیداتیو و دیابت ناشی از آن را دارند. انجبار گیاهی حاوی فلاونویید است لذا در این مطالعه تاثیر عصاره هیدروالکلی ریشه انجبار بر میزان قند خون موشهای سوری دیابتی شده با استرپتوزوتوسین ارزیابی شده است. برای انجام این مطالعه، دیابت با mg/kg30 استرپتوزوتوسین به صورت داخل صفاقی در ۱۵ موش سوری القا شد و موشها به طور تصادفی به 3 گروه تقسیم شدند و یک گروه هم به عنوان کنترل در نظر گرفته شد. گروه کنترل و دیابتی، نرمالین سالین را دریافت کردند و گروه های تیمار به ترتیب mg/kg 5/0 گلی بنکلامید و mg/kg 150 عصاره هیدروالکلی ریشه انجبار را به مدت ۱۵ روز دریافت کردند. در پایان روزهای اول، هفتم و پانزدهم مطالعه، خونگیری انجام شد و میزان گلوکز خون اندازه گیری شد. داده ها با استفاده از آزمون آنالیز واریانس یک طرفه و آزمون تعقیبی توکی به کمک نرم افزار SPSS ورژن 25تحلیل شد. در این پژوهش05/0> Pدر نظر گرفته شد. داده ها حاکی از آن بود که به دنبال القای دیابت، میزان گلوکز خون در تمامی گروه های دیابتی در روزهای اول، هفتم و پانزدهم افزایش معنی داری در مقایسه با گروه کنترل داشت(05/0>P).

کلیدواژه‌ها

عنوان مقاله [English]

The effect of hydroalcohlic of Bistort (Polygonum bistorta L) root on blood glucose levels of streptozotocin induced diabetic mice

نویسندگان [English]

  • elham salehi 1
  • majid morovatisharifabad 1
  • mahdieh karimi 2
  • mahnaz sazi zavareh 2

1 assistant professor,Department of Basic sciences, Faculty of Veterinary Medicine, Ardakan university,Ardakan,Iran

2 Department of Basic sciences, Faculty of Veterinary medicine, Ardakan university, Ardakan, Iran

چکیده [English]

Diabetes is one of the most common disease of the present century. The many side effects of the chemical drugs used to treat it increase the tendency to be treated by herbs that have fewer side effects. Plant flavonoids with their antioxidant effect have the ability to inhibit oxidative stress and diabetes. Bistort contains flavonoids, so in this study, the effect of hydroalcoholic extract of Bistort root on blood glucose in streptozotocin diabetic mice have been assessed in the present study. In this experimental study, diabetes was induced by administration of 30 mg/kg of streptozotocin intraperitoneally in 15 mice and they were randomly divided into 3 groups. One group considered as control. The control and diabetic groups received normal saline and the treatment groups received glibenclamide at dose 0.5 mg/kg and hydroalcoholic extract of Bistort root at doses of 150 μg/kg for 15 days. At the end of the first , seventh and fifteenth days of the study, blood samples were taken and blood glucose levels were measured. The data were analysed by one way Anova and Turkey's test using SPSS 25. In this study, P<0/05 was considered.In this study, following the induction of diabetes blood glucose levels in all diabetic groups in the first, seventh and fifteenth days had a significant increase compared to control group (P<0/05).

کلیدواژه‌ها [English]

  • Blood glucose
  • Diabetes mellitus
  • Mice
  • Polygonum bistorta L
  • Streptozotocin
Ahangarpour, A., Mohammadian, M., & Dianat, M. (2012). Antidiabetic Effect of Hydroalcholic Urtica dioica Leaf Extract in Male Rats with Fructose-Induced Insulin Resistance. Iranian Journal Medical Sciences, 37(3): 181–186.
Babre, N. P., Subal, D., Yalagatti, M., Gajanan, D., Hariprasath, K., & Kukkala, S. (2010). Hypolipidemic effect of hydro-alcoholic extract of Barringtonia acutangula Linn. Root extract on streptozotocin-induced diabetic rats, Journal of Pharmaceutical Science and Technology, 2(11): 368-371
Burton-Freeman, B., Brzezinski, M., Park, E., Sandhu, A., Xiao, D., & Edirisinghe, I. (2019). A Selective Role of Dietary Anthocyanins and Flavan-3-ols in Reducing the Risk of Type 2 Diabetes Mellitus: A Review of Recent Evidence. Nutrients, 11(4): 841
Coffey, T. (1994).The History and Folklore of North American  WildFlowers. Houghton Mifflin Harcourt: Boston.USA, 210-215
Custro, N., Carroccio, A., Ganci, A., Scafidi, V., Campagna, P., Prima, L. Di., & Montalto, G. (2001). Glycemic  homeostasis in chronic viral hepatitis and liver  cirrhosis. Diabetes & Metabolism, 4(1): 476-481
Damasceno, D. C., Netto, A. O., Iessi, I. L., Gallego, F. Q., Corvino, S. B., Dallaqua, B., & Rudge, M. V. C. (2014). Streptozotocin-Induced Diabetes Models: Pathophysiological Mechanisms and Fetal Outcomes, BioMed Research International, (Vol 2014)
Dastoor, R., Bakhshi, D., & Aliakbar, A. (2018). Evaluation of Total phenolic and Flavonoid content, Antioxidant capacity and Resveratrol of selected medicinal plant of northern iran. Agriculturea conspectus scientificus, 83(2): 175-180
Delima, S., Moreira, L., Affonso, V. R., Henriques, A. B., Sato, A., Esquibel, M. A., & Lago, C. L. S. (2010). Micropropagation of Polygonum acre Kunth var. aquatile (mart.) Meisn, and seasonal variation of tannins in acclimatized plans. Journal of medicinal plant research, 4(7): 573-578
Donner, H., Rau, H., Walfish, P. G., Braun, J., Siegmund, T., Finke, R., … & Badenhoop, K. (1997). CTLA4 Alanine-17 Confers Genetic Susceptibility to Graves’ Disease and to Type 1 Diabetes Mellitus 1. The Journal of Clinical Endocrinology & Metabolism, 82(1): 143-146
Farajpour, R., Sadigh-Eteghad, S., Ahmadian, N.,  Farzipour, M., Mahmoudi, J., & Majdi, A. (2017). Chronic  Administration of Rosa canina Hydro-alcoholic extract attenuates depressive-Like behavior and recognition Memory  Impairment in diabetic Mice: a possible  role of Oxidative Stress. Medical  Principles and Practice, 26(3): 245-250
Gallou, G., Ruelland, A., Legras, B., Maugendre, D.,  Allanic, H., & Cloarec, L. (1993). Plasma malondialdehyde in type 1 and type 2 diabetic patients. Clinica Chimica Acta, 214(2): 227-234
Genuth, S., Bennett, P., Buse, J., Defronzo, R., Kahn, R., Kitzmiller, J., & Zimmet, P.  (2003). Follow-up report on the diagnosis of diabetes mellitus: The Expert Committee on the Diagnosis and Classification of Diabetes Mellitus 2, Diabetes Care, 26(11): 3160– 3167
Ghelich, T., Hashemi–karouei, M., & Ghlampor–Azizi, I. (2014). Antibacterial effect of Methanolic Extraction of Polygonum Bistorta on some Bacteria. Medical laboratory Journal, 8(2): 41-47
Ghorbani, A., Rashidi, R., & Shafiee-Nick, R. (2019). Flavonoids for preserving pancreatic beta cell survival and function: a mechanistic review. Biomedecine & pharmacotherapies, (vol 111): 947–957 [Article in persion]
Graham, J. E., Stoebner May, D. G., Ostir, G. V., Snih, S. AL., Peek, M. K., Markides, K., & Ottenbacher, K. J. (2007). Health related quality of life in older Mexican Americans with diabetes: a cross-sectional study. Health and Quality of life Outcomes, 5(1): 1-7 [Article in persion]
Gupta, S., Kataria, M., Gupta, P. K., Murganandan, S., & Yashroy, R. C. (2004). Protective role of extracts of neem seeds in diabetes caused  by streptozitocin in rats. Journal of Ethnopharmacology, 90(2-3):185-189
He-yang, Y., Zhi-hua, H., Xiu-rong, W., Zhou, L., Zhi-ping, H., Wei, H., Jing, Z. (2005). Protective effect of Polygonum bistora L. n-butyl alcohol extract on myocardial ischemia/reperfusion injury in rats in a dosage dependent manner. Chinese Journal of Clinical Rehabilitation. 699
Heydari, M., Hashempur, M. H., Ayati, M. H., Quintern D, Nimrouzi, M., & Mosavat, S. H. (2015).The use of Chinese herbal drugs in Islamic medicine. Journal of Integrative Medicine, 13(6): 363-367
 Intisar, A., Zhang, L., Luo, H., Kiazolu, B. J., Zhang, R., & Zhang, W. (2012). Anticancer constituents and cytotoxic activity of methanol-water extract of Polygonum bistorta L. African Journal of Traditional, Complementary and Alternative Medicines, 10(1): 53-59
Jayaprakasam, B., Vareed, S. K., Olson, L. K., & Nair, M. G. (2005). Insulin secretion by bioactive anthocyanins and anthocyanidins in Present in fruits. Journal of  Agricultural and Food Chemistry, 53(1): 28-31
Kaneto, H., Kajimoto, Y., Miyagawa, J. I., Matsuoka, T. a., Fujitani, Y., Umayahara, Y., & Hori, M. (1999). Beneficial effects of antioxidants in diabetes possible protection of pancreatic beta-cells against glucose toxicity. Diabetes, 48(12): 2398-2406
Kato, A., Minoshima, Y., Yamamoto, J., Adachi, I., Watson, A. A., & Nash, R. J. (2008). Protective Effects of Dietary  Chamomile Tea on Diabetic Complications. Journal of  agricuitural and  Food Chemistry, 56(17): 8206-8211
Khaksari, M. (2000). Effect of nutrients containing bean and choicory on blood glucose of diabetic rats .kowsar Medical journal, 6(1): 1-8
Khalid, A., Waseem, A., Saadullah, M., Urreham, U., Khiljee, S., Sethi, A., & Murtaza, Gh. (2011). Antibacterial activity analysis of extracts of various plants against gram-positive and-negative bacteria. African Journal of Pharmacy and pharmacology, 5(7): 887-893
Khalili, R., Hasanzadeh, Sh., Jalali, A. Sh ., Shahrooze, R., Najafi, Gh., & Eimani, M. (2020). The effects of liraglutide on invitro fertilization in mice following exprimental diabetes.Qom university of medical sciences journal, 14(1): 51-60
Kiasalari, Z., Khalili, M., Roghani, M., Heidari, H., & Azizi, Y. (2013). Antiepileptic and antioxidant effect of hydroalcoholic extract of ferula assa foetida gum on pentylentetrazole-induced kindling in male mice. Basic and clinical neuroscience, 4(4): 299 -306
Kumar, M. D., Deepmala, J., Sangeeta, S. (2012). Hepatoprotective effects of polygonum bistorta and its active principles on albino rats intoxicated with carbon tetrachloride and paracetamol. Open access scientific report, 1(4): 226-230
Lecube, A., Hernandez, C., Genesca, J., Esteban, J. I., Jardi, R., & Simo, R. (2004). High prevalence of glucose  abnormalities in patients with hepatitis C virus infection: a multivariate analysis considering the liver injury. Diabetes Care, 27(5): 1171-1175
 Lenzen, S. (2008). The mechanisms of alloxanand-and streptozotocin-induced diabetes. Diabetologia, 51(2): 216-226
Malekirad, A. A., Nabiyouni, F., Vaezi, Gh., & Abdullahi, M.  (2016). The effect of ethanol extract of Alhagi camelorum on hepatic and renal functions in streptozotocin induced diabetic rat. Journal of Experimental Animal Biology, 1(5): 38-31[Article in Persian]
Manoharan, K. P., Benny, T. K., & Yang, D. (2005). Cycloartane type triterpenoids from the rhizomes of Polygonum bistorta. Phytochemistry, 66(19): 2304–2308
Morovati Sharifabad, M., Lotfi, Z., & Salehi, A. (2020). Protective effects of alcoholic extract of marshmallow (Althaea officinalis L.) on diazinon induced liver damage in adult male hamsters. Journal of Al-Zahra University, 3 (33): 153-136 [Article in Persian]
Narasimhula, G., Reddy, K. K., & Mohamed, J. (2014). The genus Polygonum (Polygonaceae): An ethnopharmacological and phytochemical perspectives: review. International Journal of Pharmacy and Pharmaceutical Sciences, 6(2): 21-45
Nathan, D. M., Buse, J. B., Davidson, M. B., Ferrannini, E., Holman, R. R., Shervin, R., & Zinman, B. (2009). Medical management of hyperglycamia in type 2 diabetes mellitus: A consensus algorithm for the initiation and adjustment of therapy .Diabetes care, 32(1): 193-203
Nickavar, B., Abou Alhasani, L., & Izadpanah, H. R. (2008). α-Amylase inhibitory activities of Six salvia species. Iranian Journal of Pharmaceutical Research, 7(4): 297-303
Ogwuru, N., & Adamczeski, M. (2000). Bioactive natural products  derived from polygonum species of plants: their  structures and mechanisms of action. Studies in Natural  Products Chemistry, 22(C): 607-642
Ozbay, H., & Alim, A. (2009). Antimicrobial Activity of Some Water Plants from the Northeastern Anatolian region  of Turkey. Molecules, 14(1): 321-328
Partovia, T., Zabihia, M. (2012). Coagulant compound from rhizomes of polygonum bistorta (Linn). Global advanced research jornal of engineering, technology and innovation. 1(6): 127-130
Rajasekaran, S., sivagnanam, K., & Subramanian, S. (2005). Antioxidant effect of Aloe vera gel extract in streptozotocin – induced diabetes in rats. pharmacol Rep, 57(1): 90-96
Sanchez, A., Schuster T. M., Burke J. M., & Kron, K.A. (2011).Taxonomy of Polygonoideae (Polygonaceae): a new tribal classification.Taxon, 60(1): 151-160
Shahabinezhad, M., Rahmani, M. R., Khaksari-Hadad, M., Sepehri, Gh., Mahmoodi, M., & Karimghasemi, E. (2007). The Effect of Licorice Root  Extract on Blood Sugar Level in Streptozotocin Induced Diabetic Rats. Journal of Rafsanjan University of  Medical Sciences, 6(4): 237-244.
Shokri, H., Farrokhi, F., Heydari, R., & Manaf Far, R. (2015). The effect of hydroalcoholic extract of rhubarb root on blood Glucose and histopathological changes of pancreas in alloxan-induced diabetic rats. Journal of Qom University of Medical Sciences, 8 (5): 20-26 [Article in Persian]
Srivastava, R.C. (2014). Family polygonaceae in India. Indian Journal of Plant Sciences, 3(2): 112-150
Szkudelski,T., Kandulska, K., & Okulicz, M. (1998). Alloxan in vivo does not only exert deleterious effects on pancreatic B cells. Physiological Research, (vol 47): 343-346
Thulesen, J., Orskov, C., Holst, J. J., & Poulsen, S. S. (1997). Short term insulin treatment  prevents the diabetogenic action of streptozotocin in rats. Endocrinology, 138(1): 62-68
Tiwari, A. K., & Rao, J. M. (2002). Diabetes mellitus and multiple therapeutic of phytochemical: present status and future prospect. current science, 83(1):30_38 .
Tripathi, B. K., Srivastava, A. K. (2006). Diabetes mellitus: complication and therapeutics. Med Sci Monit, 12(7): 130-147
Tsutsui, H., Kinugawa, S., & Matsushima, Sh.(2009). Mitochondrial oxidative stress and dysfunction in myocardial remodeling. cardiovascular research, 81(3):449-456
Whiting, D. R., Guariguata, L., Weil, C., & Shaw, J. (2011). IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes research and clinical practice, 94(3): 311-321