زیست شناسی کاربردی

شماره جاری

بر اساس شماره‌های نشریه

بر اساس نویسندگان

نمایه نویسندگان

نمایه کلیدواژه ها

براساس موضوعات

درباره نشریه

اهداف و چشم انداز

اعضای هیات تحریریه

اصول اخلاقی انتشار مقاله

بانک ها و نمایه نامه ها

پیوندهای مفید

پرسش‌های متداول

فرایند پذیرش مقالات

اخبار و اعلانات

اطلاعات آماری نشریه

راهنمای نویسندگان

جهت رفع مشکل پرداخت مالی در سامانه

راهنمای ثبت نام داروان در Publons

داوران سال 1398

داوران سال 1399

داوران سال 1400

داوران سال 1401

جداسازی و شناسایی قارچهای اندوفیت تولید کننده ال- آسپاراژیناز از گیاه مریم گلی باغی ( .Salvia nemorosa L)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار ، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

2 دانشیار، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

3 استادیار ، گروه شیمی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز

چکیده

آنزیم ال- آسپاراژیناز تجزیه آسپاراژین را به آسپارتیک اسید و آمونیاک کاتالیز کرده و یکی از مهمترین آنزیم ها در صنایع دارویی و غذایی می باشد. هدف از این مطالعه، جداسازی و شناسایی قارچ های اندوفیت تولید کننده آنزیم آسپاراژیناز از گیاه مریم گلی باغی و تعیین فعالیت این آنزیم در این قارچ ها می باشد. در این پژوهش، گونه های مختلف قارچ های اندوفیت از برگ های سالم گیاه مریم گلی باغی جداسازی گردید. سنجش کیفی آنزیم آسپاراژیناز روی محیط کشت آسپاراژین آگار و سنجش کمی آن در محیط کشت سیزپکس داکس به روش طیف نور سنجی با معرف نسلر انجام شد. بررسی های ریختی و مولکولی نشان داد جدایه ها بدست آمده به جنس های Alternaria، Stemphylium، Talaromyces و Linnemannia تعلق دارند. گونه های مختلف با ایجاد هاله صورتی تا قرمز رنگ نشان دادند قادر به تولید آنزیم آسپاراژیناز هستند. گونهAlternaria alternata با تولید U/mg 34/2 آسپاراژیناز در محیط کشت سیزپکس داکس، بهترین جدایه تولید کننده آنزیم انتخاب شد و می تواند به عنوان یک گزینه مناسب برای تولید آنزیم مطرح باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Isolation and identification of L-asparaginase-producing endophytic fungi from Salvia nemorosa L.

نویسندگان [English]

  • Samaneh Narimani 1
  • Mostafa Ebadi 1
  • Mohammad Pazhang 2
  • Saeid Mollaei 3

1 Assistant Professor, Department of Biology, Azarbaijan Shahid Madani University, Tabriz, Iran

2 Associate Professor, Department of Biology, Azarbaijan Shahid Madani University, Tabriz, Iran

3 Assistant Professor , Department of Chemistry, Azarbaijan Shahid Madani University, Tabriz، Iran

چکیده [English]

L-asparaginase is an enzyme that catalyzes the hydrolysis of asparagine into aspartic acid and ammonia and is one of the most important enzymes in the pharmaceutical and food industries. The goal of this study was the isolation and identification of asparaginase producing endophytic fungi from Salvia nemorosa and the determination of their enzyme activities. In this study, different species of endophytic fungi were isolated from healthy leaves of S. nemorosa. A qualitative assay of asparaginase enzyme was performed on asparagine agar medium and its quantitative assay was performed on Czapexdox's medium by spectroscopic method with Nessler reagent. Morphological and molecular investigations indicated that the obtained isolates belonged to genera of Alternaria, Stemphylium Talaromyces, and Linnemannia . Different species were shown to be able to produce the asparaginase by creating a pink to a red color zone. Alternaria alternata was selected as the best enzyme-producing isolate by producing 2.34 U/mg asparaginase in Czapexdox's medium, and could be considered as a potential source for enzyme production.

کلیدواژه‌ها [English]

  • L-asparaginase
  • Phylogenetic
  • Endophytic fungi
  • S. nemorosa
  • Nesslerization
مراجع
Barnett, H.L., Hunter, B.B. (1998). The Illustrated Genera of Imperfect Fungi, fourth edition. Aps Press, The American Phytopathological Society, St. Paul, Minnesota 218 p.
Bhagat, J., Kaur, A., Kaur, R., Yadav, A.K., Sharma, V., Chadha, B.S. (2016). Cholinesterase inhibitor (Altenuene) from an endophytic fungus Alternaria alternata: optimization, purification and characterization. Journal of applied microbiology, 121(4): 1015-25.
Carris, L.M., Little, C.R., Stiles, C.M. (2012). Introduction to Fungi. The Plant Health Instructor.
Chen, S., Ding, M., Liu, W., Huang, X., Liu, Z., Lu, Y., Liu, H., She, Z. (2018). Anti-inflammatory meroterpenoids from the mangrove endophytic fungus Talaromyces amestolkiae YX1. Phytochemistry, 146: 8-15.
Chen, S., Liu, Y., Liu, Z., Cai, R., Lu, Y., Huang, X., She, Z. (2016). Isocoumarins and benzofurans from the mangrove endophytic fungus Talaromyces amestolkiae possess α-glucosidase inhibitory and antibacterial activities. RSC advances,6(31): 26412-20.
Daskalova, T. (2004). On some specificities of seed formation in Salvia nemorosa (Lamiaceae). Phytologia Balcanica, 10: 79-84.
Dhanam, J.G., Kannan, S. (2013). L-asparaginase- Types, perspectives and applications. Advanced Biotech, 13(5): 1-5.
Ebadi, M., Riahi, H., Zare, R. (2014). Genetic diversity of Fusarium semitectum isolates from rice, using RAPD and REP-PCR markers. Mycologia Iranica, 1(1): 19-26.
Fang, Z.F., Yu, S.S., Zhou, W.Q., Chen, X.G., Ma, S.G., Li, Y., Qu, J. (2012). A new isocoumarin from metabolites of the endophytic fungus Alternaria tenuissima (Nees & T. Nees: Fr.) Wiltshire. Chinese Chemical Letters, 23(3): 317-20.
Fernandes, M.D., Pfenning, L.H., Costa-Neto, C.M., Heinrich, T.A., Alencar, S.M., Lima, M.A., Ikegaki, M. (2009). Biological activities of the fermentation extract of the endophytic fungus Alternaria alternata isolated from Coffea arabica L. Brazilian Journal of Pharmaceutical Sciences, 45(4): 677-85.
Fotovvat, M., Radjabian, T., Saboora, A. (2018). HPLC fingerprint of important phenolic compounds in some Salvia L. species from Iran. Records of Natural Products, 13(1).
Fu, Y., Li, C., Zhu, J., Zhang, L., Wang, Y., Chen, Q., Xu, L., Zhang, S., Fang, Y., Liu, T. (2020). A new meroterpenoid from endophytic fungus Talaromyces amestolkiae CS-O-1. Biochemical Systematics and Ecology, 93: 104186.
Gao, F.K., Dai, C.C., Liu, X.Z. (2010). Mechanisms of fungal endophytes in plant protection against pathogens. African Journal of. Microbiology Research, 4 (13): 1346-1351.
Guevara-Araya, M.J., Vilo, C., Urzúa, A., González-Teuber, M. (2020). Differences in community composition of endophytic fungi between above-and below-ground tissues of Aristolochia chilensis in an arid ecosystem. Revista Chilena de Historia Natural, 93:1-9.
Gupta, S., Schillaci, M., Walker, R., Smith, P.M., Watt, M. and Roessner, U. (2021). Alleviation of salinity stress in plants by endophytic plant-fungal symbiosis: current knowledge, perspectives and future directions. Plant and Soil, 461(1): 219-244.
Hatamzadeh, S., Rahnama, K., Nasrollahnejad, S., Fotouhifar, K.B., Hemmati, K., White, J.F., Taliei, F. (2020). Isolation and identification of L-asparaginase-producing endophytic fungi from the Asteraceae family plant species of Iran. Peer-reviewed Journal, 8: e8309.
Heydari, H.R., Chamani, E., Esmaielpour, B. (2020). Cell line selection through gamma irradiation combined with multi-walled carbon nanotubes elicitation enhanced phenolic compounds accumulation in Salvia nemorosa cell culture. Plant Cell, Tissue and Organ Culture (PCTOC), 142(2): 353-367.
Hyde, K.D., Soytong, K. (2008). The fungal endophyte dilemma. Fungal Diversity, 33: 163-173.
Jalgaonwala, R.B., Mohite, B.V., Mahajan, R.T. (2011). Natural products from plant associated endophytic fungi: a review. Journal of Microbiology and Biotechnology Research, 1: 21-32.
Imada, A., Igarasi, S., Nakahama, K., Isono, M. (1973). Asparaginase and glutaminase activities of microorganisms. Journal of General Microbiology, 76: 85-99.
Johnson, J.M., Ludwig, A., Furch, A.C., Mithöfer, A., Scholz, S., Reichelt, M., Oelmüller, R. (2019). The beneficial root-colonizing fungus Mortierella hyalina promotes the aerial growth of Arabidopsis and activates calcium-dependent responses that restrict Alternaria brassicae–Induced disease development in roots. Molecular Plant-Microbe Interactions, 32(3):351-63.
Lacap, D.C., Hyde, K.D., Liew, E.C.Y. (2003). An evaluation of the fungal ‘morphotype’ concept based on ribosomal DNA sequences. Fungal Divers, 12: 53-66.
Larson, R.A., Fretzin, M.H., Dodge, R.K., Schiffer, C.A. (1998). Hypersensitivity reactions to L-asparaginase do not impact on the remission duration of adults with acute lymphoblastic leukemia. Leukemia, 12: 660-665.
Moharram, A., Zohri, A.N., Seddek, N. (2016). L-Asparaginase production by endophytic fungi isolated from Withania Somnifera in Egypt. SS International Journal of Multidisciplinary Research, 2: 30-40.
Oettgen, H.F., Stephenson, P.A., Schwartz, M.K., Leeper, R.D., Tallal, L., Tan, C.C., et al. (1970). Toxicity of E. coli L-asparaginase in man. Cancer, 25: 253-278.
Ozimek, E., Hanaka, A. (2021). Mortierella Species as the Plant Growth-Promoting Fungi Present in the Agricultural Soils. Agriculture, 11(1): 7.
Pieters, R., Hunger, S.P., Boos, J., Rizzari, C., Silverman, L., Baruchel, A., et al. (2011). L-Asparaginase treatment in acute lymphoblastic leukemia. Cancer, 117(2): 238-249.
Perez, Y., Schenck, N. (1990). A unique code for each species of VA mycorrhizal fungi. Mycologia, 82: 256-260.
Pour Nouroozi, R. (2015). Determination of protein concentration using bradford microplate protein quantification assay. International Electronic Journal of Medicine, 4: 11-17.
Sandhu, S.S., Kumar, S., Aharwal, R.P. (2014). Isolation and identification of endophytic fungi from Ricinus communis linn and their antibacterial activity. International Journal of Research in Pharmacy and Chemistry, 4 (3): 611-618.
Selim, K.A., El-Beih, A.A., AbdEl-Rahman, T.M., El-Diwany, A.I. (2012). Biology of endophytic fungi. Current Research in Environmental & Applied Mycology, 31-82. 
Shukla, A.K., Yongam, Y., Tripathi, P. (2012). Distribution of endophytic fungi in different parts of rudraksh (Elaeocarpus sphaericus) plants. Microbes: diversity and biotechnology. New Delhi: Daya Publishing House, 37-42.
Strobel, G., Daisy, B. (2003). Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews, 67 (4): 491–502. 
Sudharshana, T.N., Venkatesh, H.N., Nayana, B., Manjunath, K., Mohana, D.C. (2019). Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation. Mycology,10(1): 40-8.
Tabei, S. M., Alizadeh, A. (2018). Phytochemical constituents and antimicrobial activity of Salvia. Bangladesh Journal of Botany, 47(4): 847-854.
Takeda, Y., Zhang, H., Matsumoto, T., Otsuka, H., Oosio, Y., Honda, G., Tabata, M., Fujita, T., Sun, H., Sezik, E. and Yesilada, E. (1997). Megastigmane glycosides from Salvia nemorosa. Phytochemistry, 44(1): 117-120.
Tareke, E., Rydberg, P., Karlsson, P., Eriksson, S., Tornqvist, M. (2002). Analysis of acrylamide, a carcinogen formed in heated food stuffs. Journal of Agricultural and Food Chemistry, 50(17): 4998-5006.
Ulubelen, A. (2003). Cardioactive and antibacterial terpenoids from some Salvia species. Phytochemistry, 64(2): 395-399.
Weber, D. (2009). Endophytic fungi, occurrence and metabolites. In: Anke, T., Weber, D. (eds). The Mycota. Vol. XV, Physiology and Genetics selected basis and applied aspects springer- Verlag, Berlin, Germany, pp. 153-195.
Werber, G., Ahlke, E., Nowak-Göttl, U., Jürgens, H., Verspohl, E,J., Boos, J. (1995). Asparaginase activities in vitro are highly sensitive to different buffer conditions. In: Büchner T., Schellong G., Ritter J., et al., editors. Acute Leukemias VI. Berlin, Heidelberg: Springer, Berlin, Heidelberg, 512-516.
Wu, W.B., Yue, G.C., Huang, Q.L., Sun, L.L., Zhang, W. (2014). A new compound from an endophytic fungus Alternaria tenuissima. Journal of Asian Natural Products Research,16(7): 777-82.
Xu, F., Oruna-Concha, M.J., Elmore, J.S. (2016). The use of asparaginase to reduce acrylamide levels in cooked food. Food Chemistry, 210: 163-171.
Zhao, J., S. W. Bao, G. P. Ma, and X. H. Wu. (2016). Characterization of Alternaria species associated with watermelon leaf blight in Beijing municipality of China. Journal of Plant Pathology, 135-138.
 
 
 
زیست شناسی کاربردی
دوره 34، شماره 4 - شماره پیاپی 70
بهمن 1400
صفحه 180-194
فایل ها
هم رسانی
ارجاع به این مقاله
آمار