نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد زیست فناوری میکروبی، دانشکده علوم پایه، دانشگاه مراغه، آذربایجان شرقی، ایران

2 استاد، دانشکده علوم زیستی، دانشگاه الزهرا، تهران، ایران استاد، دانشکده علوم پایه، دانشگاه مراغه، آذربایجان شرقی، ایران

3 دانشیاردانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

پروبیوتیک‌ها میکروارگانیسم‌های زنده‌ای هستند که در پیشگیری و درمان از بیماری‌ها نقش مهمی دارند. با توجه به اهمیت مخمر‌های پروبیوتیک، هدف این تحقیق جداسازی مخمر با ویژگی‌های پروبیوتیکی است. نمونه‌برداری از ‌قسمت‌های مختلف پوست انسان انجام شد. توانایی رشد مخمر جدا شده در غلظت‌های 3/0، 5/0 و 1درصد اکسگال، غلظت‌های 2، 4 و 6 درصد NaCl، pH های 2، 4، 8/5، 9 و 11 و دمای 37، 40 و 42 درجه سلسیوس بررسی شدند. فعالیت آنزیم‌های آمیلاز، پروتئاز، پکتیناز، سلولاز، لیپاز، اوره‌آز، کاتالاز، سیتراتاز و همولیزین نمونه‌ جداشده بررسی شد. این مخمر توانایی رشد در 3/0درصد اکسگال، غلظت‌های 2، 4 و 6 درصد NaCl، pH های2، 4، 8/5، 9 و 11و همچنین دمای 37 درجه سلسیوس را داشت. مخمر جدا شده ویژگی اولیه پروبیوتیک را دارد و می‌تواند در مطالعات بعدی مورد استفاده قرار گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Isolation of yeast with probiotic properties from human skin

نویسندگان [English]

  • Mohadeseh Ghaffari 1
  • Farshad Darvishi 2
  • sonbol nazeri 3

1 Master of Microbial Biotechnology, Faculty of Basic Sciences, Maragheh University, East Azerbaijan, Iran

2 Professor, Faculty of Biological Sciences, Alzahra University, Tehran, Iran Professor, Faculty of Sciences, University of Maragheh, East Azarbaijan, Iran

3 assocaite prof biotechnology dept., Faculty of agriculture Bu-Ali Sina University, Hamedan, Iran

چکیده [English]

Probiotics are living microorganisms that have an important role in prevention and treatment of diseases. The aim of this study was isolate yeast with probiotic properties due to the importance of probiotic yeasts. Sampling was performed from different parts of human skin. Growth ability of isolated yeast at concentrations of 0.3, 0.5 and 1% oxgall, 2, 4 and 6% NaCl, pHs of 2, 4, 5.8, 9 and 11 and temperature 37, 40 and 42°C were investigated. The activity of amylase, protease, pectinase, cellulase, lipase, urease, catalase, citratase and hemolysin was investigated. The yeast was able to grow in 0.3% oxgall, concentrations of 2, 4 and 6% NaCl, pHs of 2, 4, 5.8, 9 and 11 and also at a temperature of 37°C. The isolated yeast has the primary properties of probiotics and can be used in future studies.

کلیدواژه‌ها [English]

  • Probiotic
  • yeast
  • Enzyme activity
  • Salt tolerance
  • Oxgall
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