نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکترای پژوهشکده زیست فناوری و مهندسی زیستی، دانشگاه صنعتی اصفهان، اصفهان، ایران
2 محقق درکمیسیون ملی بیوتکنولوژی دمشق، دمشق، سوریه
چکیده
تولید پلی‌ساکارید‌های لاکتیکی (با توجه به گراس بودن آن‌ها) می‌تواند به عنوان جایگزین ارزشمندی برای پلی‌ساکارید‌های میکروبی تولید شده فعلی باشند. در این تحقیق به بررسی منابع کربن و نیتروژن مناسب برای تولید اگزو‌پلی‌ساکارید‌ها (EPSs) توسط لوکونوستوک دکسترانیکوم (Leuconostoc dextranicum) که یک باکتری گراسی است، پرداخته شد. نتایج بررسی-های انجام شده نشان داد که گلوکز (Glu) در مقایسه با قند‌های دیگر و پپتون در مقایسه با منابع نیتروژن دیگر باعث تولید EPSs بیشتری در محیط کشت شدند. لذا در ادامۀ تحقیق تنها از گلوکز و پپتون جهت بهینه کردن شرایط تولید EPSs استفاده شد. به منظور بررسی تأثیر و بهینه سازی شرایط تولید EPS، سه پارامتر دما، pH و زمان انکوباسیون، هر کدام در پنج سطح انتخاب شده و به طور جداگانه بهینه شدند. سپس برای دستیابی به سطوحی از متغیر‌های مستقل که در نتیجه به کارگیری آن‌ها بهترین محصول از واکنش به دست می‌آمد، بهینه سازی انجام شد. بر اساس نتایج به‌دست آمده، دمای انکوباسیون°C 40، pH برابر 5/5 و زمان تخمیر 48 ساعت به عنوان بهترین شرایط برای تولید آزمایشگاهی EPSs انتخاب شدند. به طور خلاصه نتایج حاصل از این تحقیق نشان داد که می‌‌توان از باکتری بومی لوکونوستوک دکسترانیکوم و محیط کشت ارزان قیمت شیر پس چرخ (Skimmed milk) برای تولید این محصول با ارزش استفاده کرد.
کلیدواژه‌ها
عنوان مقاله [English]
Optimization of EPS production conditions by Leuconostoc dextranicum isolated from traditional Syrian cheese
نویسندگان [English]
- Muhannad Haj Mustafa 1
- Bassam Al-oklah 2
- Ghasem Ashouri 1
1 Translation results PhD student at the Research Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran
2 Researcher at the National Biotechnology Commission of Damascus, Damascus, Syria
چکیده [English]
Increasing demand for industrial applications of various natural polymers has led to increased attention to the use of exopolysaccharides made by microorganisms. Since most lactic acid bacteria (LAB) are food-grade microorganisms with GRAS status (Generally Recognized As Safe), the use of the secreted EPS as natural alternatives to produce all-natural food products without additives from LAB has received increased attention. In this research, carbon and nitrogen sources suitable for the production of exopolysaccharides (EPSs) by Leuconostoc dextranicum, as GRAS bacterium, were investigated. The results showed that glucose (Glu) produced more EPSs in the medium compared to other sugars and peptone compared to other nitrogen sources. Therefore, only glucose and peptone were used to optimize the production conditions of EPSs. In order to investigate the effect and optimization of EPS production conditions, three parameters of temperature, pH and incubation time, each was selected at five levels and optimized separately. Then, optimization was performed to determine the levels at which independent variables attain the best production. Results showed that the best laboratory condition for EPSs production would be yielded at the incubation temperature of 40 °C, pH of 5.5 and fermentation time of 48 hours. In summary, the results of this study showed the potential of Leu. dextranicum as a native microorganism and the cheap culture medium of skimmed milk for producing such a valuable product.
کلیدواژه‌ها [English]
- EPSs
- Lactic acid bacteria
- Leu. dextranicum
- Skimmed milk
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