تاثیر کاربرد کود مرغی حاوی انروفلوکساسین بر تنفس، بیوماس میکروبی و جمعیت برخی باکتری‌های مفید خاک

محمدزاده, مهسا; قاسمیان رودسری, فروزان; حسنی, اکبر; زمانی, عباسعلی

doi:10.22051/jab.2019.20737.1234

نوع مقاله : مقاله پژوهشی

نویسندگان

¹ گروه علوم محیط زیست، دانشکده علوم، دانشگاه زنجان

² دانشگاه زنجان،دانشکده علوم، گروه زیست شناسی

³ گروه علوم خاک، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

⁴ دانشگاه زنجان، دانشکده علوم، گروه علوم محیط زیست

https://doi.org/10.22051/jab.2019.20737.1234

چکیده

غلظت آنتیبیوتیکها در خاک بر اثر اضافه کردن کود مرغی آلوده به آنتیبیوتیک افزایش مییابد و ممکن است جامعه میکروبی خاک را تحت تاثیر قرار دهد. هدف این پژوهش تاثیر اضافه نمودن کود مرغی محتوی انروفلوکساسین به خاک بر جمعیتAzotobacter spp.، Pseudomonas fluorescens و Pseudomonas putida و همچنین بیوماس میکروبی و تنفس میکروارگانیسمهای خاک است. یک آزمایش در آزمایشگاه به مدت 45 روز با 7 تیمار و سه تکرار انجام شد. این آزمایش شامل کود مرغی تازه و کمپوست شده محتوی آنتیبیوتیک دردو سطح غلظتی بالا ( mg kg-12/26) و پایین (mg kg-123/4) و کود مرغی بدون آنتیبیوتیک بر مبنای طرح کرتهای به‌طورکامل تصادفی انجام شد. نتایج نشان داد کاربرد کود مرغی حاوی آنتیبیوتیک منجر به کاهش جمعیت Azotobacter spp.(%28)، Pseudomonas fluorescens(%73) و Pseudomonas putida(%6/14) در خاکها شد. کمپوست کردن کود مرغی نیز اگرچه اثر منفی آن را کمتر کرد اما همچنان کاهش جمعیت وجود داشت. کاربرد کود مرغی تازه بدون آنتی بیوتیک سبب افزایش 127 درصدی تنفس میکروبی خاک نسبت به شاهد شد اما بین سایر تیمارهای مختلف کاربرد کود مرغی تفاوت معنیدار دیده نشد. کاربرد کود مرغی بدون آنتیبیوتیک کربن بیوماس میکروبی را به طور معنیداری افزایش داد (%95). وجود آنتیبیوتیک در کود مرغی تاثیر منفی بر غلظت کربن بیوماس میکروبی داشت. به طور کلی رهاسازی و انتشار آنتیبیوتیکها در خاک بر اثر افزودن کود مرغی به مزارع حتی اگر قبل از مصرف کمپوست شده باشد، باعث کاهش رشد گروههای باکتریایی مفید خاک شد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of chicken manure containing enrofloxacin on soil respiration, microbial biomass, and population of some beneficial bacteria

نویسندگان [English]

Mahsa Mohammadzadeh ¹
forouzan Ghasemian Roudsari ²
Akbar Hassani ³
Abbasali Zamani ⁴

¹ Department of Envirnmental Science, Faculty of Science, University of Zanjan, Zanjan, I. R. Iran

² Department of Biology,Faculty of Science, University of Zanjan, Zanjan, Iran,

³ Soil Science Department, Faculty of agriculture, University of zanjan, Zanjan, Iran

⁴ Department of Envirnmental Science, Faculty of Science, University of Zanjan, Zanjan, I. R. Iran

چکیده [English]

The concentration of antibiotics in the soil increases with the addition of fresh or composed chicken manure containing antibiotics and may affect the microbial community of the soil. The purpose of this study was to evaluate the effect of adding enrofloxacin containing chicken manure to soil on the population of Azotobacter spp, Pseudomonas putida and Pseudomonas fluorescens, as well as microbial biomass and respiration of soil. An experiment was conducted in a laboratory for 45 days with 7 treatments and three replications including fresh and composted chicken manure containing enrofloxacin in two levels of high (26.2 mg kg-1) and low (4.23 mg kg-1) concentrations and manure without any antibiotic based on a completely randomized design. The results showed that the application of chicken manure containing enrofloxacin reduced the population of Azotobacter spp (28 percent), Pseudomonas fluorescens (73 percent) and Pseudomonas putida (14.6 percent) in the soils. However, the composting of chicken manure, although reducing its negative effect, still led to a decline in population. Application of fresh chicken manure led to a 127 percent increase in microbial respiration of the soil compared to control but between other treatments there was no meaningful difference. The use of chicken manure without antibiotic significantly increased the microbial biomass (95 percent). The presence of high concentration of enrofloxacin in poultry manure had a negative effect on the microbial biomass increase, especially at the beginning of the period.

کلیدواژه‌ها [English]

Azotobacter
Compost
Pseudomonas fluorescens
Pseudomonas putida

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زیست شناسی کاربردی

تاثیر کاربرد کود مرغی حاوی انروفلوکساسین بر تنفس، بیوماس میکروبی و جمعیت برخی باکتری‌های مفید خاک

مراجع

مراجع

دوره 32، شماره 4 - شماره پیاپی 62
اسفند 1398
صفحه 144-163

تاثیر کاربرد کود مرغی حاوی انروفلوکساسین بر تنفس، بیوماس میکروبی و جمعیت برخی باکتری‌های مفید خاک

مراجع

مراجع

دوره 32، شماره 4 - شماره پیاپی 62اسفند 1398صفحه 144-163

دوره 32، شماره 4 - شماره پیاپی 62
اسفند 1398
صفحه 144-163