نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته گروه علوم دامی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

2 عضو هیات علمی/ گروه علوم دامی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران

چکیده

هدف از انجام این آزمایش، تعیین ترکیبات شیمیایی و بررسی روش­های تانن­زدایی در برگ گردوی ایرانی (Junglans regia L.) و تخمین فراسنجه­های تخمیری آن­ها به­روش تولید گاز بود. روش­های مذکور شامل روش­های خیساندن، جوشاندن، پرکولاسیون و سوکسله بودند. نتایج نشان­داد که کاهش مقدار تانن در تمامی روش­های فرآوری نسبت به تیمار شاهد معنی­دار بود. همچنین روش خیساندن سبب افزایش معنی­دار پروتئین خام، انرژی خام، الیاف نامحلول در شوینده خنثی و الیاف نامحلول در شوینده اسیدی نسبت به سایر تیمارها شد. فرآوری به­روش خیساندن نسبت به سایر تیمارها سبب افزایش معنی­دار تولید گاز تجمعی و تولید گاز از بخش نامحلول (b) و افزایش غیرمعنی­دار نرخ تولید گاز (c)، انرژی قابل متابولیسم و هضم­پذیری ماده آلی شد (05/0<p). لذا با توجه به نتایج به­دست آمده، روش خیساندن و استفاده از آب­مقطر نسبت به اتانول، روش مناسبی جهت استخراج تانن از برگ گردو و مصرف آن در خوراک نشخوارکنندگان می­باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of different methods of tannin extraction from the leaves of Iranian walnut (Juglans regia L.) and their effects on chemical composition and fermentative parameters in vitro

نویسندگان [English]

  • behzad amir 1
  • saeed sobhani rad 2

چکیده [English]

The aim of this study was to determine the chemical composition and evaluation of extraction methods of tannin from leaves of Iranian walnut (Juglans regia L.) and estimation of fermentative parameters by gas production technique. Mentioned methods include maceration method, boiling method, percolation method, and Soxhlet method. The results showed that the number of tannins in all the processing methods was reduced significantly compared to the control treatment (p < 0.05). Crude protein, crude energy, natural detergent fiber and acid detergent fiber were also increased to compare to other treatments, significantly (p < 0.05). Soaking Process compared to other treatments increased significantly cumulative gas production, the fermentable fraction (b) of gas production and also increased insignificantly the parameters of rate (c) of gas production, metabolizable energy, and digestibility of organic matter. Therefore, by comparing the chemical composition and fermentative characteristics of the processed products, it can be concluded maceration method and use distilled water compared to ethanol, is a suitable method for the extraction of tannin and its use in ruminants' feedstuff.

کلیدواژه‌ها [English]

  • walnut
  • tannin removal methods
  • gas production
  • fermentation characteristics
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