بررسی اثرات ضد قارچی اسانس مرزه بختیاری، دارچین و رازیانه بر رشد سه گونه قارچ بیماری‌زای گیاهی در شرایط آزمایشگاهی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد بیماری شناسی گیاهی، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ایلام

2 استادیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ایلام

3 کارشناس ارشد، گروه باغبانی، دانشکده کشاورزی، دانشگاه ایلام

چکیده

متابولیت‌های ثانویه گیاهی، به دلیل داشتن خواص ضدمیکروبی و آنتی‌اکسیدانی جایگاه ویژه‌ای در کنترل عوامل بیماری‌زا گیاهی دارند. در همین راستا اثر ضدقارچی سه اسانس مرزه بختیاری ((Satureja bachtiarica ، دارچین ((Cinamomum zeylancium و رازیانه ( (Foeniculum vulgare بر رشد سه گونه قارچ مهم بیماری‌زای گیاهی Fusarium oxysporumو Fusarium solani و Pyrenephora graminea مورد مطالعه قرار گرفت. بعد از خشک شدن گیاهان در سایه، اسانس روغنی نمونه ها به روش تقطیر با آب و با استفاده از دستگاه کلونجر جداسازی شد. اثرات ضد قارچی اسانس‌های مذکور در غلظت‌های 300،200،100 و 400 پی‌پی‌ام (ppm) بر روی قارچ‌ها در شرایط آزمایشگاه آزمون شد. نتایج نشان داد که در کلیه اسانس‌ها، بهترین غلظت برای توقف رشد پرگنه جدایه‌های F. oxysporum و F. solani، غلظت 400 پی‌پی‌ام بود. از طرفی در رابطه با قارچP. graminea ، مهار کامل رشد پرگنه قارچ توسط اسانس دارچین در تمامی غلظت ها مشاهده شد در صورتی‌که اسانس مرزه فقط در دو غلظت 300 و 400 پی‌پی‌ام منجر به مهار کامل رشد میسیلیومی قارچ شده، نتایج این پژوهش نشان داد اسانس هر سه گیاه نقش موثری در کنترل عوامل بیماری زای قارچی داشته و می توانند در کنترل بیولوژیک آنها مورد استفاده قرار گیرند.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of antifungal effects of essential oil Savory, Cinnamon and Fennel on the growth of three species of plant pathogenic fungi in vitro

نویسندگان [English]

  • afsaneh aliaran 1
  • khoshnood nourollahi 2
  • zahra mehdi shahivand 3

1 M.Sc., Department of Plant Protection, Faculty Agriculture, Ilam University

2 Assistant Professor, Department of Plant protection, Faculty Agriculture, Ilam University

3 MSc, Department of Horticulture, Faculty of Agriculture, Ilam University

چکیده [English]

Herbal extracts are used to control pathogens, due to their antimicrobial and antioxidant properties of secondary metabolites. In this regard, antifungal effect of Satureja Bachtiarica Bunge., Foeniculum vulgar mill. and Cinamomum zeylancium Nees. on the growth of three important species of plant pathogenic fungi including, Fusarium oxysporum, Fusarium solani, and Pyrenephora graminea, was studied in this research. After drying the plants in the shade, essential oils were isolated through the steam distillation method using Clevenger. Antifungal effects of above-mentioned essential oils in concentrations of 100, 200, 300 and 400 ppm was investigated under laboratory conditions revealed that the essence showed inhibitory effects on tested fungi. The best concentration for full stop growing F. oxysporum, F. solani is 400 ppm at all essences, and observed Inhibition of mycelial growth of P. graminea by Cinnamon essential oil at all concentrations, Whereas concentrations of 300 and 400 ppm of Savory, leads to have been complete inhibition of P. graminea fungal mycelium growth. The results showed all three plant essential oils play an important role in controlling fungal pathogens and they can be used for biological control.

کلیدواژه‌ها [English]

  • Essence
  • Fusarium oxysporum
  • Fusarium solani
  • Pyrenephora graminea
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