اثر سالیسـیلیک اسـید بر برخی از شـاخصهای رشـد و آنزیمهای آنتی اکسیدان چرخه ی گلوتاتیون آسـکوربات تحت کشـت در شیشـه گیاه گوجه فرنگی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استاد گروه زیست شناسی دانشگاه اصفهان

2 کارشناسی ارشد، گروه زیست شناسی دانشگاه اصفهان

چکیده

سالیسیلیک اسید به‌عنوان یک هورمون گیاهی، موجب تولید گونه‌های فعال اکسیژن مانند H2O2 به‌عنوان یک مولکول پیام‌رسان می‌شود. این هورمون در غلظت‌های زیاد با تجمع گونه‌های فعال اکسیژن موجب تنش‌های اکسیداتیو می‌گردد. گیاهان برای مقابله با این تنش اکسیداتیو از مکانیسم‌هایی مانند آنتی‌اکسیدان‌های آنزیمی و غیر آنزیمی استفاده می‌کنند. تاکنون اثر سالیسیلیک اسید بر چرخه گلوتاتیون آسکوربات در گیاه گوجه‌فرنگی‌ به‌عنوان یک گیاه مدل به‌خوبی مشخص نشده است. در این مطالعه گیاه گوجه‌فرنگی‌ Lycopericon esculentum Mill)) به مدت 4 هفته در محیط کشت حاوی سالیسیلیک اسید در غلظت‌های 0001/0، 001/0، 01/0، 1/0 و 5/0 میلی مولار سالیسیلیک اسید کشت داده شد و اثر سالیسیلیک اسید بر شاخص‌های رشدی، میزان H2O2، MDA و فعالیت آنزیم‌های CAT، APX، GR، DHAR، SOD و ترکیبات آسکوربات، دی ‌‌هیدرو ‌آسکوربات، پرولین و قند محلول بررسی شد. با توجه به نتایج، بیشترین میزان RWC، طول ساقه و طول ریشه در غلظت 01/0 میلی مولار سالیسیلیک اسید مشاهده شد. میزان فعالیت دو آنزیم APX و CAT در غلظت‌های پائین کاهش یافت و در ادامه، با افزایش غلظت تیمار سالیسیلیک اسید، افزایش معنی‌داری را نسبت به نمونه شاهد نشان داد. میزان MDA، H2O2، پرولین، قند محلول و آنزیم‌های چرخه گلوتاتیون- آسکوربات، با افزایش میزان سالیسیلیک اسید افزایش معنی‌داری را از خود نشان دادند. این نتایج نشان می‌دهد که گیاه برای مقابله با تنش اکسیداتیو ناشی از افزایش گونه‌های فعال اکسیژن، ترکیباتی مانند پرولین، قند محلول و آنتی‌اکسیدان‌های آنزیمی و غیر آنزیمی را تغییر و افزایش می‌دهد. در این مطالعه، غلظت 01/0 میلی مولار سالیسیلیک اسید با بیشترین میزان شاخص‌های رشدی و کمترین میزان تنش به‌عنوان غلظت بهینه در این گیاه معرفی می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The impact of salicylic acid on some growt parameters and antioxidant enzymes of glutathione-ascorbate cycle in tomato plant under in vitro culture

نویسندگان [English]

  • Aliakbar Ehsanpour 1
  • Mohamad Sadegh Maleki 2

1 Professor of Biology, Isfahan University

2 M.Sc. Department of Biology, Isfahan University

چکیده [English]

Salicylic acid is a plant hormone which accumulates ROS production like H2O2 as a signal
molecule and in toxic concentration cause oxidative stress with hyper accumulation of ROS.
Plants use enzymatic antioxidant like glutathione-ascorbate cycle enzymes and non-enzymatic
antioxidant such as ASA and DHASA against oxidative stress. The effect of salicylic
acid on ascorbate –glutathione cycle antioxidants in tomato plants as amodel plant is not
clear Tomato (Lycopericon esculentum Mill) plants cultured in vitro for 4 weeks on MS medium
containing salicylic acid (SA) treatment in concentrations of 0.0001, 0.001, 0.01, 0.1
and 0.5 mM SA.... In this study, the effect of salicylic acid investigated on growth parameters,
H2O2 and MDA content and activity of SOD, CAT, APX, GR, DHAR enzymes and also
ASA, DHASA, proline and total soluble sugar. The results showed that maximum amount
of RWC, root and shoot length reached in 0.01 mM SA. H2O2 and MDA content increased
under SA treatment. APX and CAT activity initially decrease in low concentrations of SA and
then increased in toxic level (0.1 and 0.5 mM SA). Activity of GR, DHAR, SOD, ASA, DHASA,
proline and total soluble sugar gradually increased compared with control plants. These
results indicate that plants for defense against oxidative stress cause by ROS production
change and induce proline, total soluble sugar and ascorbate-glutathione cycle enzymes
and compounds. In this study, the concentration of 0.01 mM SA with the highest growth indices
and the least amount of stress as the optimal concentration in the plant is introduced.
 

کلیدواژه‌ها [English]

  • ROS
  • Salicylic acid
  • Tomato
  • Glutathione-Ascorbate cycle
 

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