نوع مقاله : مقاله پژوهشی

نویسندگان

چکیده

در مطالعه حاضر با توجه به اهمیت گیاه سورگوم شیرین اندامهای مختلف گیاه شامل قطعات برگ، ریشه و بذر بالغ از گیاهان رشد یافته در شرایط کشت این ویترو در محیط کشت MS روی محیط کشت های حاوی ترکیب مختلفی از هورمونهای IBA, NAA, 2,4-D, Kinetin,  کشت گردید. در بین قطعات کشت شده تنها بخشهای تازه روئیده از بذر بالغ جوانه زده روی محیط کشت حاوی 2,4-D و Kinetin توانست پس از 1 تا 3 ماه کالوس تولید کند.

کلیدواژه‌ها

عنوان مقاله [English]

Callus production from mature seeds of Sorghum bicolor L.

نویسندگان [English]

  • Fariba Hadian
  • Ali akbar Ehsan pour
  • Abbas Almodares

چکیده [English]

In the present study, based on the importance of sorghum plant different explants including leaf, root segments and mature seed fron in vitro grown plant on MS medium were cultured on the same medium containing Kinetin, 2,4-D, IBA and NAA. Among explants only segments from germinated seeds were able to produce callus after 1-3 months.

کلیدواژه‌ها [English]

  • Callus
  • sweet sorghum
  • in vitro culture
1. احسانپور، ع. الف. و امینی، ف.، کشت سلول و بافت گیاهی. چاپ 2، انتشارات جهاد دانشگاهی اصفهان. 181 صفحه، 1382.
 
2. Balakin, K. V., Savchuk, N. P. and Tetko, I. 2006. In silico approaches to prediction of aqueous and DMSO solubility of drug-like compounds: trends, problems and solutions.  Current Medicinal Chemistry. 13, 223-238, 2006.
3. Baskaran, P., Rajeswari, B. R. and Jayabalan, N. 2006. Development of an in vitro regeneration system in Sorghum [Sorghum bicolor (L) Moench] using root transverse thin cell layers. Turkish Journal of Botany. 30, 1-9, 2006.
4. Bhaskaran, S. and Smith, R. H., Control of morphogenesis in Sorghum by 2,4-dichlorophenoxy acetic acid and cytokinins. Annual Botany. 64, 217-224, 1989.
5. Cai, T., Daily, B. and Butler, L., Callus induction and plant regeneration from shoot portions of mature embryos of high tannin Sorghums. Plant Cell Tissues and Organ Culture. 9, 245-252., 1987.
6. Gamez-Pastrana, R., Martinez-Ocampo, Y., Beristain, C. I. and Gonzalez-Arnao, M. T., An improved cryopreservation protocol for pineapple apices using encapsulation-vitrification. Cryo-Letters. 25, 405-414, 2004.
7. Hagio, T., Varietal difference of plant regeneration from callus of Sorghum mature seed. Breeding Science. 44, 121-126, 1994.
8. Hagio, T., Adventitious shoot regeneration from immature embryo of Sorghum. Plant cell Tissue and organ culture. 68, 65-72, 2002.
9. Heslop-Harrison, Y. and Shivanna, K. R. The evaluation of pollen quality and a further appraisal of the fluorochromatic (FCR) test procedure. Theoretical and Applied Genetics. 67, 367-375, 1984.
10. Kishore, S. N., Visarada, K. B. R. S., Lakshmi, A. Y., Pashupatinath, E., Rao, S. V. and Seetharama, N., In vitro culture methods in Sorghum with shoot tips the explant Material. Plant Cell Reports. 25, 174-182, 2006.
 
 
 
 
11. Kuruvinashetti, M. S., Patil, V. M., Sumangala, B. and Maheshwar, H. High frequency plant regeneration from embryogenic callus cultures in genus Sorghum. Indian journal of agricultural Sciences. 68, 27-28, 1998.
12. Laopaiboon, L., Thanonkeo, P., Jaisil, P. and Laopaiboon, P. Ethanol production from sweet Sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae. World Journal Microbiol Biotechnol. 23, 497-1501, 2007.
13. Ma, H., Gu, M. and Liang, G. H. Plant regeneration from cultured immature embryos of Sorghum bicolor (L.) Moench. Theory of Applied Genetics. 73, 389-394, 1987.
14. Mifflin, M. Crop improvement in the 21st century. Experimental Botany. 51, 1-8, 2002.
15. Mishra, A. and Khurana, P., Genotype dependent somatic embryogenesis and regeneration from leaf base cultures of Sorghum bicolor. Journal of Plant Biochemistry and Biotechnology. 12, 53-56, 2003.
16. Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473-497, 1962.
17. Pola, S., Saradamani, N. and Ramana, T. Enhanced shoot regeneration in tissue culture studies of Sorghum bicolor. Agricultural Technology. 3, 275-286, 2007.
18. Pola, S. R. and Mani, N. S. Somatic embryogenesis and plantlet regeneration in Sorghum bicolor (L.) Moench, from leaf segments. Cell and Molecular Biology. 5, 99-107, 2006.
19. Rose, J. B., Dunwell, J. M. and Sunderland, N. Anther culture of Sorghum bicolor  (L.) Moench. Effect of panicle pretreatment, Anther incubation temperature and 2,4-D concentration. Plant Cell, Tissue and Organ Culture. 6, 15-22, 1986.
20. Seetharama, N., Sairam, R. V. and Rani, T. S. Regeneration of Sorghum from shoot tip cultures and field performance of the progeny. Plant Cell Tissue and Organ Culture. 61, 169-173, 2000.
21. Smith, C. W. Structure and Chemistry of the Sorghum caryopsis. In: Sorghum: Origin, History, Technology and Production: John Wiley and Sons, Inc. Texas. pp: 721-723, 2000.
22. Zhong, H., Wang, W. and Sticklen, M. 1998. In vitro regeneration of Sorghum bicolor (L.) Moench: efficient plant regeneration from shoot apices. Journal of Plant Physiology. 153, 719-726.