رنگ زدایی رنگ آزوی قرمز کنگو توسط باکتری های جداسازی شده از پساب کارخانجات نساجی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار،دانشگاه آزاد اسلامی واحد رشت

2 کارشناسی ارشد،دانشگاه آزاد اسلامی واحد رشت

چکیده

رنگ های آزو بدون هیچگونه پیش تصویه ای به فاضلاب وارد می شود و محیط زیست را آلوده می سازد. برای جلوگیری از آلودگی منابع آسیب پذیر، حذف آلاینده های رنگی مهم است. روش های سنتی تصویه پرهزینه می باشد و مشکلات عملیاتی شدن دارد. روش زیستی یک روش ارزان برای احیای رنگ ها به حساب می آید. برای این منظور نمونه های پساب از نقاط آلوده به رنگ برای جداسازی باکتری تجزیه کننده، جمع آوری شد. در مراحل بعد باسیل هوازی گرم مثبت جداسازی شد که توانایی چشمگیری در حذف رنگ را داشت. پارامترهای متغییر مختلفی مانند منبع کربن و نتروژن، حجم تلقیح، دما و اسیدیته برای بهینه سازی رنگ زدایی قرمز کنگو با استفاده از باکتریها، استفاده شد. نتایج نشان داد پارامترهای اسیدیته، منبع نیتروژن و حجم تلقیح در رنگ زدایی قرمز تاثیرگذارتر بودند. رنگ زدایی زمانیکه زایلوز به عنوان منبع کربن و عصاره مخمر به عنوان منبع نیتروژن بود، افزایش یافت. درصد حجم تلقیح بهینه برای رنگ زدایی پنج درصد بود. اسیدیته بهینه جهت حذف رنگ توسط باکتری در شرایط اسیدی (pH=5) و دمای بهینه رنگ زدایی برابر با 25 درجه سانتیگراد بود. تحت شرایط بهینه رنگ زدایی در حدود 100% صورت پذیرفت. میزان رنگ زدایی بالا و شرایط آسان استفاده نشان داد که باکتری جداسازی شده پتانسیل بالایی در تصویه بیولوژیکی فاضلاب رنگرزی دارد و برای این امر می تواند استفاده شود. نتایج گزارش شده، نیاز به تحقیقات بیشتری برای اثبات سودمندی ایزوله جداسازی شده جهت تجزیه کاربردی رنگ ها مانند استفاده در تصویه پساب، دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Study on azo dye congo red biodegredation by isolated bacteria from textile effluent

نویسندگان [English]

  • Massoumeh Anvari 1
  • Hossein Pourkazem 2

1 Associate Professor, Islamic Azad University, Rasht Branch

2 Master of Science (MSc), Islamic Azad University, Rasht Branch

چکیده [English]

Azo dyes are released into wastewater streams without any pretreatment environments. To prevent contamination of our vulnerable resources, removal of these dye pollutants is of great importance. Traditional methods of treatment are found to be expensive and have operational problems. Biological decolourization has been investigated as a method to degrade azo dyes .
For this purpose, wastewater samples were collected from dye-contaminated sites to isolate Congo red decolourizing bacteria. one strains were obtained with potential for Congo red decolourization. On primary screening, an aerobic gram positive bacillus was found to have maximum observable Congo red decolourization activity.
Different parameters such as various carbon source, nitrogen source, inoculum size, temperature and pH were optimized for decolourization of Congo red by using bacterial isolates. pH, nitrogen source and inoculum size were found to be most effective in dye decolorization.
Enhanced decolourization was observed in presence of xylose as a carbon source and yeast extract as a nitrogen source.The optimum of inoculum size was found to be 5%. The optimum pH obtained for decolourization of Congo red by bacteria strains was 5.0. The optimum temperature was found to be 250C. under optimal conditions maximum decolourization percentage was about 100%.
High decolorization extent and facile conditions show the potential for this bacterial strain to be used in the biological treatment of dyeing effluents. The results reported here warrant further investigation to establish the usefulness of these isolates for biodegradation application such as waste water treatment.

کلیدواژه‌ها [English]

  • "Textile wastewater"
  • "Congo red"
  • "B. Subtlis"
  • "Biodegradation"
  • "Decolorization"
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