نوع مقاله : مقاله پژوهشی
نویسندگان
1 دکترای میکروبیولوژی، دانشکده علوم زیستی، دانشگاه شهید بهشتی
2 دانشیار گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه شهید بهشتی
3 استاد، دانشگاه شهید بهشتی، مرکز تحقیقات گیاهان و مواد اولیه دارویی، گروه فیتوشیمی، تهران، ایران
چکیده
ظهور مقاومت های
دارویی در بین میکروارگانیسم های بیماریزا نشان دهنده نیاز به کشف آنتی بیوتیک های
جدید است. در جستجوی یک میکروارگانیسم تولید کننده ماده ضد میکروبی جدید از خاک
های آلوده به نفت اطراف دزفول یک سویه subsp. faecalis Alcaligenes faecalis جداسازی گردید. عوامل موثر
در تولید ماده ضد میکروبی به روش یک فاکتور در هر زمان تعیین گردید که بر اساس آن به
ترتیب 9 pH =، دما °C
35، % 34/0NH4Cl، % 2 استات سدیم و % 02/0 K2HPO4
بیشترین تاثیر را داشت. بر اساس این نتایج غلظت استات و NH4Cl،
دور همزن و زمان فرمانتاسیون به روش سطح پاسخ با طراحی مرکب مرکزی بهینه شدند.
بهترین شرایط % 88/1 استات، % 29/0 NH4Cl، دور همزن rpm 3 و زمان 7/6
روز تعیین گردید که باعث افزایش %47 در تولید گردید. همچنین مشخص گردید که در بین متغیر
ها برهم کنش وجود دارد.
کلیدواژه‌ها
عنوان مقاله [English]
Optimization of fermentation condition for antibiotic production by Alcaligenes faecalis with response surface methodology
نویسندگان [English]
- Mohammad Yaghoobi Avini 1
- Gholamhossein Ebrahimipour 2
- Alireza Ghassempour 3
چکیده [English]
The emergence of drug resistant pathogens indicates
the need to discovery of new antibiotics. In the search for a new antibiotic
producing microorganism, an Alcaligenes faecalis subsp. faecalis strain was isolatedfrom oil
polluted soils near Dezful. One factor at a time was used to find important
variables in antimicrobial compound production and upon its result pH = 9,
temperature 35 ˚C, NH4Cl 0.34%, sodium acetate 2% and K2HPO4
0.02% have greatest effect on production, respectively. Acetate and NH4Cl
concentration, shaker revolution and fermentation time were selected for
optimization by response surface method with central composite design. The best
result achieved by sodium acetate 1.88%, NH4Cl 0.29%, shaker 3 rpm
and fermentation time 6.7 day which had 47% higher production than one factor
at a time method. Results also indicated strong interaction between some
variables.
کلیدواژه‌ها [English]
- Alcaligenes faecalis
- one factor at a time
- response surface method
- central composite design
- oil pollution
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