نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار. گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان

2 دانشجوی کارشناسی ارشد فیزیولوژی گیاهی، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گیلان

3 دانشجوی کارشناسی ارشد محقق مرکز تحقیقات توتون گیلان

چکیده

در این تحقیق اثرات چهار غلظت کلر آب آبیاری (10، 20، 40 و 80 میلی گرم در لیتر 2CaCl) به عنوان غلظت های تنش زا بر فعالیت آنزیم های پراکسیداز، آسکوربات پراکسیداز و پلی فنل اکسیداز، محتوای مالون دآلدهید و پروتئین کل در سه رقم توتون گرمخانه ای (کوکر، ویرجینیا و کنتاکی) مورد بررسی قرار گرفت. نتایج نشان داد افزایش غلظت کلر تا غلظت 80 میلی گرم بر لیتر موجب کاهش معنی دار فعالیت آنزیم پراکسیداز در رقم کوکر می شود. در کلیه ارقام مورد مطالعه بیشترین فعالیت آسکوربات پراکسیداز درغلظت 40 و کمترین فعالیت در غلظت 80 میلی گرم بر لیتر بوده و فعالیت آسکوربات پراکسیداز در کنتاکی و ویرجینیا درغلظت 40 افزایش ودر غلظت 80 میلی گرم بر لیتر در هر سه رقم کاهش یافت. مقدار مالون دآلدهید در رقم کوکر تنها درغلظت 20 افزایش یافته و در سایر غلظت ها اختلاف معنی داری مشاهده نشد. در رقم کنتاکی، مقادیر مالون دآلدهید در کلیه تیمار ها در مقایسه با شاهد به طور معنی داری کاهش یافت. در ویرجینیا در تیمار 20 مقدار مالون دآلدهید کاهش یافته و در سایر تیمار ها اختلاف معنی دار مشاهده نشد. مقادیر پروتئین کل در دو رقم کنتاکی و ویرجینیا با افزایش غلظت کلر، کاهش یافت.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of different chlorine concentrations on antioxidant enzymes activity in three varieties of flue-cured tobacco

نویسندگان [English]

  • Akbar Norastehnia 1
  • Sahar Babajani 2
  • Jannat Sarmad 1
  • Mahyar Moshtagi 3

چکیده [English]

Salt stress due to chlorine is one of the limiting factors in plant growth. An increasing of antioxidative enzymes activity is a defensive response of salt stresses. In this research, the effects of four chlorine concentrations in irrigation water (10, 20, 40, 80 mg/l CaCl2) on the activities of enzymes peroxidase, ascorbate peroxidase and polyphenol oxidase and malondialdehyde content, total leaf protein, and peroxidase zymogram in three greenhouse tobacco varieties were investigated. The results showed that an increase in chlorine concentration up to 80 mg/l, peroxidase enzyme activity decreased in Cooker. The highest and lowest ascorbate peroxidase activities of all stressed varieties were seen in 40 and80 mg/l treatments, respectively. polyphenol oxidase activity induced in Kentucky and Virginia by 40 mg/l treatment and reduced under 80 mg/l treatment in all three varieties. The measurement of malondialdehyde content in Cooker showed that there was no significant difference among all treatments, except in 20 mg/l concentration which was increased. Malondialdehyde content decreased significantly in Kentucky in all treatments compared to control. Malondialdehyde content of Virginia also showed no significant difference, except in 20 mg/l concentration which was reduced. Total protein content of Kentucky and Virginia was reduced with increasing of chlorine concentration.

کلیدواژه‌ها [English]

  • Chlorine
  • Tobacco
  • Malondialdehyde (MDA)
  • Peroxidase (POD)
  • Polyphenol Oxidase (PPO)

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