نوع مقاله : مقاله پژوهشی

نویسنده

استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه بوعلی سینا همدان

چکیده

ترمیم زخم فرآیند پیچیده­ای هست و مطالعات نشان داده است که شمار زیادی از تولیدات طبیعی همچون داروهای گیاهی تاثیرات مهمی در تنظیم فرایند ترمیم زخم دارند. مطالعات فارماکولوژیکی نشان داده است که گیاه بابا آدم دارای فعالیت ضد­التهابی، ضد­میکروبی و ضد تکثیری می­باشند هدف از این مطالعه بررسی تاثیرات عصاره گیاه بابا آدم بر فرآیند ترمیم زخم می­باشد. مدل زخم برشی در رَت نژاد ویستار ایجاد شد. حیوانات به سه گروه 6 تایی: کنترل ، حامل و گروه تیمار  تقسیم شدند. اندازه زخم، تعداد سلولهای التهابی، انباشت رشته­های کلاژن و وسعت بافت التیامی ارزیابی شد. تعداد سلولهای التهابی از لحاظ آماری به روش one-way ANOVA، به همراه تستTukey’s Post hocبررسی شدند. نتایج نشان دادند که تعداد سلولهای التهابی به طور معنی­داری در گروه تیمار نسبت به گروههای کنترل وحامل کاهش پیدا کرد. همچنین نتایج نشان داد که وسعت بافت التیامی در گروه تیمار کاهش یافت و میزان انباشت رشته­های کلاژن در گروه تیمار به شکل معنی داری نسبت به گروه کنترل و حامل افزایش یافت. مطالعه ما نشان داد که عصاره آبی-اتانولی گیاه بابا­آدم تاثیرات مفیدی در بهبود مدل زخمهای برشی دارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effects of hydro-ethanolic extract of Arctium lappa leaves on wound healing

نویسنده [English]

  • Siamak Yari

Asistant professor Bu-Ali Sina University, Faculty of Science, Department of Biology

چکیده [English]

Wound healing is a complex process and studies show that many natural products such as herbal drugs have important effects on modulating of this process. Pharmacological studies indicated that Arctium lappa have anti-inflammatory, antimicrobial and anti-proliferative activities. The aim of this study was to evaluate the effects of Arctium lappa extract on wound healing process. Excision wound model was created in Wistar rats. The animals divided into three groups (n=6): control, vehicle and treatment group. Wound size, Number of inflammatory cells, collagen deposition and scar tissue area were studied. Number of inflammatory cells, statically were analyzed by one-way ANOVA followed by the Tukey’s post hoc test. Results show that number of inflammatory cells were significantly decreased in treated group compared to control and vehicle groups. Scar tissue reduced in treated groups and collagen deposition significantly increased in extract treated groups. Our study demonstrates that Arctium lappa have beneficial effects on healing of excision wound model. 

کلیدواژه‌ها [English]

  • Arctium lappa
  • Excision wound
  • Inflammatory cell
  • Wistar rat
Abad, A.N.A., Nouri, M.H.K., Tavakkoli, F. (2011). Effect of Salvia officinalis hydroalcoholic extract on Vincristine-induced Neuropathy in mice. Chinese Journal of Natural Medicines. 9 (5) 354-358.
Altomare, M., Nascimento, A. P., Romana-Souza, B., Amadeu, T. P. and Monte-Alto-Costa, A. (2009). Ultrasound accelerates healing of normal wounds but not of ischemic ones. Wound Repair and Regeneration. 17: 825–831.
Ashcroft, G.S., Dodsworth, J., van Boxtel, E., Tarnuzzer, R.W., Horan, M.A., Schultz, G.S., Ferguson, M.W. (1997). Estrogen accelerates cutaneous wound healing associated with anincrease in TGF-𝛽1 levels. Nature Medicine. 3:1209–1215.
Awale, S., Lu, J., Kalauni, S.K., Kurashima, Y., Tezuka, Y.,Kadota, S., Esumi, H.(2006). Identification of arctigenin asan antitumor agent having the ability to eliminate the toleranceof cancer cells to nutrient starvation. Cancer Research. 66:1751–1757.
Beckert, S., Haack, S., Hierlemann, H., Farrahi, F., Mayer, P., Konigsrainer, A., Coerper, S. (2007). Stimulation of steroid-suppressed cutaneous healing by repeated topical application of IGF-I: different mechanisms of action based upon the mode of IGF-I delivery. Journal of Surgical Research. 139: 217–21.
Chiang, L.C., Chiang, W., Chang, M.Y., Ng, L.T., Lin, C.C. (2002). Antiviral activity ofPlantago major extracts and related compounds in vitro. Antiviral Research. 55:53–62.
Cragg, G. M., Newman, D.J. (2002). Drugs from nature: past achievements, future prospects. Pp. 23-37. In: M. M. Iwu and J. C. Wootton (eds). Advances in Phytomedicine, vol.1, Elsevier.
Gál, P., Vidinský, B., Toporcer, T., Mokrý, M., Mozeš, S., Longauer, F., Sabo, J. (2006). Histological assessment of the effect of laser irradiation on skin wound healing in rats. Photomedicine and Laser Surgery. 24: 480–8.
Hirose, M., Yamaguchi, T., Lin, C., Kimoto, N., Futakuchi, M., Kono, T., Nishibe, S., Shirai, T. (2000). Effects of arctiin on PhIP-induced mammary, colon and pancreatic carcinogenesis infemale Sprague–Dawley rats and MeIQx-induced hepatocarcinogenesis in male F344 rats. Cancer Letter. 155: 79–88.
Kim, B.H., Hong, S.S., Kwon, S.W., Lee, H.Y., Sung, H., Lee, I.J., Hwang, B.Y., Song, S., Lee, C.K., Chung, D., Ahn, B., Nam, S.Y., Han, S.B., Kim, Y. (2008). Diarctigenin, a ligninconstituent from Arctium lappa, down-regulated zymosan-induced transcription of inflammatory genes through suppression of DNA binding ability of nuclear factor-kappa B in macrophages. Journal of Pharmacology and Experimental Therapeutics. 327:393–401.
Kim, Y. S., Cho, I. H., Jeong, M. J., Jeong, S. J., Nah, S. Y., Cho, Y. S., Bae, C. S. (2011). Therapeutic Effect of Total Ginseng Saponin on Skin Wound Healing. Journal of Ginseng Research. 35(3), 360–367.
Knott, A., Reuschlein, K., Mielke, H., Wensorra, U., Mummert, C., Koop, U., Kausch, M., Kolbe, L., Peters, N., Stäb, F., Wenck, H. and Gallinat, S. (2008). Natural Arctium lappa fruit extract improves the clinical signs of aging skin. Journal of Cosmetic Dermatology. 7: 281–289.
Kondo,T., Ishida, Y. (2010). Molecular pathology of wound healing. Forensic Science International. 203: 93-98.
Kyriakides, T.R., Tam, J. W.Y., Bornstein, P. (1999). Accelerated wound healing in mice with a disruption of the Thrombospondin 2 Gene. Journal of Investigative Dermatology. 113: 782-787.
Lin, C.C., Lin, J.M., Yang, J.J., Chuang, S.C., Ujiie, T. (1996.) Anti-inflammatory and radicalscavenge effects of Arctium lappa. The American Journal of Chinese Medicine. 24:127–137.
Lin, X.C., Liu, C.Y., Chen K.S., Li, G.G. (2004). Extraction and contentcomparison of chlorogenic acid in Arctium lappa L. leavescollected from different terrain and its restraining bacteria test. Natural Product Research and Development. 16:328–330.
Maver, T., Maver, U., Stana Kleinschek, K., Smrke, D. M., Kreft, S. (2015). A review of herbal medicines in wound healing. International Journal of Dermatology. 1-12.
Mitsuo, M., Nobuo, Y., Katsuya, T. (2005). Inhibitory compounds of alpha glucosidase activity from Arctium lappa L. Journal of Oleo Science. 54:589–594.
Miyamoto, K., Nomura, M., Sasakura, M., Matsui, E., Koshiura, R., Murayama, T., Furukawa, T., Hatano, T., Yoshida, T., Okuda, T. (1993) Antitumor-activityof oenothein-B, a unique macrocyclic ellagitannin. Jpn Journal of Cancer Research. 84:99–103.
Morita, T., Ebihara, K., Kiriyama, S. (1993). Dietary fiber and fat derivatives prevent mineral-oil toxicity in rats by the samemechanism. Journal of Nutrition. 123:1575–1585.
Park, S.Y., Hong, S.S., Han, X.H., Hwang, J.S., Lee, D., Ro, J.S., Hwang, B.Y. (2007). Lignans from Arctium lappaand their inhibition of LPS-induced nitric oxide production. Chemical and Pharmaceutical Bulletin. 55:150–152.
Pereira, J.V., Bergamo, D.C., Pereira, J.O., França Sde, C., Pietro, R.C., Silva-Sousa, Y.T. (2005). Antimicrobialactivity of Arctium lappa constituents against micro organisms commonly found in endodontic infections. Brazilian Dental Journal. 16:192–196.
Phillipson, J. D. (2001). Phytochemistry and medicinal plants. Phytochemistry. 56: 237–243.
Singer, A.J., Clark, R.A. (1999). Cutaneous wound healing. N. the new England Journal of Medicine. 341: 738–746.
Wang, B.S., Yen, G.C., Chang, L.W., Yen, W.J., Duh, P.D. (2007). Protective effects ofburdock (Arctium lappa Linne) on oxidation of low-densitylipoprotein and oxidative stress in RAW 264.7 macrophages. Food Chemistry. 101:729–738.