بررسی فیتوشیمیایی و فعالیت‌های پاداکسایشی در غددزیرزمینی و برگ‌های Leontice leontopetalum وL. armeniaca از ایران

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد سیستماتیک – اکولوژی، گروه زیست، دانشگاه ارومیه

2 دانشیارگروه زیست شناسی، دانشگاه ارومیه

چکیده

هدف از این مطالعه تعیین محتوای تام آلکالوئیدی، فنلی، فلاوونوئیدی و نیز بررسی میزان فعالیت پاداکسایشی، به روش بررسی فعالیت پاداکسایشی آهن احیا شده FRAP))، قدرت احیاکنندگی و جمع آوری رادیکال آزاد سوپراکسید در برگ­ها و غددزیرزمینی گونه­های L. leontopetalum و  L. armeniaca بود. نتایج نشان داد که در هر دو گونه محتوای آلکالوئید، فنل و فلاوونوئید تام وFRAP در برگ­ها از غددزیرزمینی بیشتر ونیز در L. leontopetalum بیشتر ازL. armeniaca بود.برگ و ریشه L. leontopetalum به ترتیب بیشترین (05/0± 146/1) و کمترین (03/0±889/0) قدرت احیا را نشان دادند. در همه نمونه­ها درصد مهار رادیکال سوپراکسید بیشتر از 27 درصد بود. درصد مهار رادیکال سوپراکسید برای L. armeniaca در هر دو اندام بیشتر از گونه­ی دیگر بود. بین FRAP وقدرت احیاکنندگی با محتوای فنلی و فلاوونوئیدی یک رابطه­ی قابل ملاحظه مشاهده شد. محتوای آلکالوئیدی با FRAP یک رابطه نسبتا ضعیف ولی با قدرت احیا یک رابطه­ی قوی داشت. با توجه به نتایج این مطالعه، به نظر می­رسد که جنس Leontice می­تواند یکی از منابع خوب پاداکساینده­های طبیعی باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of Phytochemical Compounds and Antioxidant Activities in Iranian Leontice leontopetalum and L. armeniacaTubers and Leaves

نویسندگان [English]

  • samira Shokatyari 1
  • rashid Jamei 2

1 MSc of Systematic, Department of Biology, University of Urmia

2 Associate Professor, Department of Biology, University of Urmia

چکیده [English]

The purpose of this study was to determine total alkaloid and phenolic contents and antioxidant activities by Ferric Reducing Power (FRAP), reducing power and scavenging of superoxide radical of tuber and leaf of L. Leontopetalum and L. armeniaca. The results revealed that in both species, total alkaloid and phenolic contents and FRAP of leaf were the greater than tuber, also in both organs was: leontopetalum > armeniaca. The highest and lowest reducing power was found in leaves and tubers of L. leontopetalm (1.146±0.055, 0.889±0.037) respectively. In all cases, free radical superoxide scavenging activity was greater than 27%. Radical superoxide scavenging activity in leaf and tuber of L. armeniaca were higher than L. leontopetalum. The correlation (R2) of antioxidant activities, FRAP and reduction power, with, phenolic contents indicatedsignificant correlations except for radical superoxide scavenging activity. Alkaloid content was only significantly associated with reducing power. The results obtained from this study show that Leontice can be a source of natural antioxidant

کلیدواژه‌ها [English]

  • Alkaloid
  • antioxidant activity
  • Flavonoid
  • Leontice
  • Phenolic compound
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