نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد، گروه میکروبیولوژی باشگاه پژوهشگران جوان و نخبگان، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان
2 استادیارگروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی، اصفهان
3 استاد گروه زیست شناسی، دانشکده علوم، دانشگاه اصفهان، اصفهان
4 دانشگاه ازاد اسلامی واحد فلاورجان
چکیده
استفاده از میکروارگانیسم ها در زمینه ی تولید مواد با ارزش از نظر اقتصادی و کاربردی، در سال های اخیر توجه زیادی را به خود جلب کرده است. از جمله این مواد با ارزش، روغن تک یاخته و پلی الکل ها با قابلیت کاربرد در عرصه های مختلف صنعتی می باشند. روغن تک یاخته در زمینه تولید سوخت زیستی و پلی الکل ها نظیر زایلیتول، در صنایع غذایی و دارویی بسیار ارزشمند هستند. در این پژوهش تولید بالای روغن میکروبی در مخمر Rhodotorulamucilaginosaبررسی شد و آنالیز روغن تولید شده با تکنیک FTIR Spectroscopy انجام شد. علاوه بر آن تولید زایلیتول توسط این مخمر در محیط دارای زایلوز و آنالیز زایلیتول به دست آمده با استفاده از تکنیک رنگ سنجی صورت گرفت. بهینه سازی شرایط تولید روغن میکروبی و زایلیتول با استفاده از روش تاگوچی انجام داده شد و نتایج با ارزشی فراهم آمد. این سویه در شرایط اپتیمم شامل g/L 100 گلوکز، 72 ساعت انکوباسیون، 5= pH، دمای ºC25 و 180= rpm به میزان بالای تولید لیپید معادل g/L 21/10 رسید. علاوه بر آن در محیط دارای 140 گرم بر لیتر زایلوز، میزان بالای تولید زایلیتول معادل g/L 99/54 به دست آمد. از یافته های حاصل از این پژوهش می توان دریافت که مخمرهای بومی دارای پتانسیل بالای تولید مواد با ارزش بوده و با فراهم آوردن شرایط محیطی مناسب، می توان مخمر را به سمت تولید محصول مورد نظر هدایت کرد.
کلیدواژه‌ها
عنوان مقاله [English]
Optimization of SCO and xylitol production in the oleaginous yeast Rhodotorula mucilaginosa
نویسندگان [English]
- Marjan Enshaeieh 1
- Mahboobeh Madani 2
- Azadeh Abdoli 2
- Iraj Nahvi 3
- Forough Asgary Karchegany 4
1 MSc of Microbiology, Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
2 Assistant Professor of Medical Mycology, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
3 Professor of Biotechnology, Department of Biology, Science Branch, Esfahan University, Esfahan, Iran
چکیده [English]
The application of microorganisms in the production of economically valuable products has attracted a great attention in recent years. These valuable materials such as single cell oil and poly-alcohols have potential for application in various industrial fields. Single cell oil in biodiesel production and poly-alcohols such as xylitol in the food and pharmaceutical industries, are valuable. In this study high production of SCO was evaluated and analyzing of SCO was done by FTIR spectroscopy. In addition, xylitol production by the yeast, in the media containing xylose was done and xylitol was analyzed by using colorimetric method. Optimization of microbial oil and xylitol production was done by Taguchi method and valuable results were obtained. This strain was reached to SCO production and dry biomass of 10.21 g/L in optimum condition including 100 g/L glucose, 72 h incubation time, pH = 5, temperature of 25 ºC and rpm=180. In addition, in the medium with 140 g/L of xylose, xylitol production was 54.99 g/L. The results of this study can show that there are native yeasts with high potential of producing valuable materials which can be directed to produce the desired products by providing appropriate medium condition.
کلیدواژه‌ها [English]
- Rhodotorula mucilaginosa
1- Altamirano A, Vazquez F, Figueroa L; (2000) Isolation and identification of xylitol- producing yeastsfrom agricultural residues. Folia Microbiological 45: 255-258.
2- Angerbauer C, Siebenhofer M, Mittelbach M, Guebitz G.M; (2008) Conversion of sewage sludge in to lipids by lipomyces starkeyi for biodiesel production. Bioresour.Technol 99: 3051-3056.
3- Baishan F, Hongwen C, Xiaolan X, Ning W, Zongding H; (2003) Using genetic algorithms coupling neural networks in a study of xylitol production: medium optimization.Process Biochemistry 38: 979-985.
4- Beopoulos A, Mrozova Z, Thevenieau F, Dall M.T.L, Hapala I, Papanikplaou S, Chardot T, Nicaud J.M; (2008) control of lipid accumulation in the yeast Yarrowia lipolytica. American Society for Microbiology 74: 7779-7789.
5- Bok S.H, Demain A; (1977) An improved colorimetric assay for polyols. Annual Review Biochemistry81: 18-20.
6- Bura R, Vajzovic A, Doty SL; (2012) Novel endophytic yeast Rhodotorulamucilaginosa strain PTD3I: production of xylitol and ethanol. Journal of Industrial Microbiology and Biotechnology 39(7): 1003-1011.
7- Cheng K.K, Ling H.Z, Zhang J.A, Ping W.X, Huang W, Ge J.P, Xu J.M; (2010) Strain isolation and study onprocess parameters for xylose- to- Xylitol bioconversion. Food Biotechnology 24(1):1606- 1611.
8-Dai C, Tao J, Xie F, Dai Y.J, Zhao M; (2007)Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity. African Journal of Biotechnology 6: 2130-2134.
9-Deak T, Chen J, Beuchat L.R; (2000) Molecular characterization of Yarrowialipolytica and Candida zeylanoides isolated from poultry. Applied and Environmental Mirobiology 66: 4340-4344.
10-Easterling E.R, French W.T, Hernandez R, Licha M; (2009) The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rodotorulaglutinis. Bioresour.Technol 100: 356-361.
11-Economou CN, Aggelis G, Pavlou S, Vayenas DV; (2010) Modeling of single cell oil production under nitrogen-limited and substrate inhibition condition. Biotechnology Bioengineering 108(5): 1049-1055.
12-Enshaeieh M, Abdoli A, Madani M, Bayat M; (2015) Recycling of lignocellulosic waste materials to produce high-value products: single cell oil and xylitol. Int. J. Environ. Sci. Technol 12:837–846.
14-Enshaeieh M, Abdoli A, Nahvi I, Madani M; (2013)Production and optimization of microbial oil from oleaginous yeasts Yarrowialipolytica DSM70562 & native yeast Geotrichum Bl.NewCellular&MolecularBiotechnology Journal 12 :15-25..[In Persian] (a)
15-Enshaeieh M, Abdoli A, Nahvi I, 2013, Medium optimization for biotechnological production of single cell oil using Yarrowia lipolytica M7 and Candida sp. Journal of cell and molecular research 5(1):17-23.(b)
16-Enshaeieh M, Abdoli A, Nahvi I, Madani M, 2013, Selection and optimization of single cell oil production from Rodotorula 110 using environmental waste as substrate. Journal of cell and molecular research 4(2):1-10.(c)
17-Enshaeieh M, Abdoli A, Nahvi I, Madani M; (2012)Bioconversion of grass hydrolyzate in to single cell oil and biodiesel using Rhodotorula mucilaginosa.Zist Fanavari microbi 4(13): 39-46.[In Persian]
18-EL- Batal A.I, Khalaf S.A; (2004) Xylitol production from corn cobs hemicellulosie hydrolysate by Candida tropicalis immobilized cells in hydrogel copolymer carrier. Journal of Agricultural Biological 6: 1066-1073.
19-El-Fadaly, El-Ahmady N, Marvan EM; (2009) Single cell oil production by an oleaginous yeast strain in a low cost cultivation medium. Research Journal of Microbiology 4(8): 301-313.
20-Elumalai S, Sakthivel R, Ganesh Kumar S; (2011) Ultra Structural and Analytical Studies of Biodiesel ProducingMicroalgae (Chlorella vulgaris andSenedesmis sp.) Collected fromTamil Nadu. India. Current Botany 2(6): 19-25.
21-European Standard EN 14078: Liquid petroleum products –Determination of fatty acid methyl esters (FAME) in middledistillates – Infrared spectroscopy method.
22-Fakas S, Papanikolaou S, Galiotou-Panayotou M, Komaitis M, Aggelis G; (2008) Biochemistry and biotechnology of Single Cell Oil. University of Patras 38-60 pp.
23-Fei Q, Chang H, Chang H.N, Shang L, Choi J.D.R, Kim N, Kang J.W; (2010) The effect of volatile fatty acid as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production. Bioresource Technology 102: 2695-2701.
24-Granstrom TB, Izumori K, Leisola M; (2007) A rare sugar xylitol PartI: the biochemistry and biosynthesis ofxylitol. Applied Microbiology and Biotechnology2: 277-281.
25-Karatay SE, Donmez G ; (2010) Improving the lipid accumulation properties of the yeast cells for biodiesel production using molasses. Bioresource Technology 101(20): 7988-7990.
26-Katre G, Joshi CH, Khot M, Zinjarde S, Ravikumar A; (2012)Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel. AMB Express 2: 36-55.
27-Khot M, Kamat S, Zinjarde S, Pant A, Chopade B, Ravikumar A; (2012) Single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel. Microbial Cell Factories 11:1-13.
28-Kraisintu P, Yongmanitchai W, Limtong S; (2010) Selection and optimization for lipid production of a newly isolated oleaginous yeast, Rdodosporidium toruloides DMKU3-TK16 44: 436-445.
29-Leesing R, Baojungharn R; (2011) Microbial Oil production by isolated oleaginous yeast Torulaspora globosa YU5/2,World academy of science. Engineering and Technology 76: 799-803.
30-Li Y.H, Liu B, Sun Y, Zhao Z.B, Bai F.W; (2005) Screening of oleaginous yeasts for broad-spectrum carbohydrates assimilation capacity. Progress in Biotechnology 25: 39-43.
31-LifHolgerson P, StecksenBlicks C, Sjostrom I, Oberg M, Twetman S; (2006) Xylitol concentration in salivaand dental plaque after use of various xylitol-containing products,Caries Research40: 393-397.
32- Lin-Vien D, Colthup N.B, Fateley W.G, Grasselil J.G; (1991) The Handbook ofInfrared andRaman Characteristic Frequencies of Organic Molecules. Academic Press, Inc. United Kingdom 141pp.
33- Liu G.Q, Lin Q.L, Jin X.C, Wang X.L, Zhao Y; (2010) Screening and fermentation optimization of microbial lipid-producing molds from forest soils. African journal of microbiology Research 4(14): 1462-1468.
34- Martan J.M., Felipe M.G.A, Silva A.J.B, Junior P.A; (2006) Evaluation of the activated charcoals and adsorption conditions used in the treatment of sugarcane bagasse hydrolysate for xylitol production. Journal of Chemical and Engineering 23(1): 9-21.
36- Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M; (2009) Biodiesel production from oleaginous microorganisms. Renewable Energy 34:1-5.
37-Millier GL; (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Annual Review Chemistry 31: 426-428.
39-Pan L.X, Yang D.F, Shao L, Li W, Chen G.G, Liang Z.Q; (2009) Isolation of oleaginous yeasts from the soil and studies of their lipid-producing capacities, ISSN, Food technol.Biotechnol 47: 215-220.
40-Papanikolaou S; (2008) 1,3-Propanediol and citric acid production from glycerol containing waste discharged after bio-diesel manufacturing process. Current Topics on Bioprocesses in Food Industry 2: 381-399.
41-Ratledge C; (2002) Regulation of lipid accumulation in oleaginous micro-organisms. Biochemical society 6:1047-1050.
42-Rao RS, Bhadra B, Shivaji S; (2007) Isolation and characterization of xylitol- producing yeasts from the gutofcolleopteran insects. Current Microbiology 5: 441-446.
43-Rao R.S, Jyothi C.P, Rao L.V; (2008) Biotechnological production of xylitol by mutant Candida tropicalis OMV5: process optimization using statistical approach. Indian Journal of Biotechnology 7: 218-224.
44-Raschke D, Knorr D; (2009) Rapid monitoring of cell size, vitality and lipid droplet development in oleaginous yeast Waltomyces lipofer. Journal of Microbiologocal Methods 79: 178-183.
45-Saha B.C, Bothast R.J; (1999) Production of xylitol by Candida peltata. Journal of Industrial Microbiology and Biotechnology 22: 633- 636.
46- Saxena R.K, Anand P, Saran S, Isar J; (2009) Microbial production of 1,3-propanediol: recent developments and emerging opportunities. Biotechnol Adv 27: 895-913.
47-Syed M.A, Singh S.K, Pandey A, Kanjilal S, Prasad R.B.N; (2006) Effects of various process parameters on the production of α-Linolenic acid in submerged fermentation. Food technology and Biotechnology 44: 282-287.
48- Vajzovic A, Bura R, Kohlmeier K, Doty S.L; (2012) Novel endophytic yeast Rhodotorula mucilaginosa strain PTD3 II: production of xylitol and ethanol in the presence of inhibitors. J Ind Microbiol Biotechnol 39: 1453-63.
49-Vakhlu J, Kour A; (2006) Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electronic Journal of Biotechnology 9: 69-85.
50-Vijayakumar S, Kumutha K, Santhana KP, Gopal H; (2010) Effect of carbon sources on lipid and biomass production by oleaginous yeast cultures. Tamil Nadu Agriculture university. Coimbatore 1-3pp.
51-Zagustina N.A, Rodionova N.A, Mestechkina N.M, Shcherbukhin V.D, Bezborodov A.M; (2001) Xylitolproduction by a culture of Candida guilliermondii2581. Applied Microbiology and Biotechnology 37(5): 489-492.
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