نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکتری تخصصی، گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 دانشیار، گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار گروه کشاورزی، سازمان پژوهش‌های علمی و صنعتی ایران (IROST)، تهران، ایران

4 استاد گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

5 دانشیار گروه میکروب شناسی، دانشکده علوم زیستی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

10.22051/jab.2021.36402.1426

چکیده

زیرالنون یکی از مهمترین و فراوانترین سموم قارچی است که باعث آلودگی مواد غذایی و خوراک دام و طیور شده و آسیب‌‌های جدی به انسان و دام وارد می‌کند. پژوهش حاضر به بررسی اثر جاذب آلی حاوی چهار سویه لاکتوباسیلوس Lactobacillus) ) بومی ایران و دیواره سلولی مخمر ساکارومایسس سرویزیه (Saccharomyces cerevisiae) بر کاهش مایکوتوکسین زیرالنون پرداخته است. با استفاده از روش کروماتوگرافی مایع با کارایی بالا (high-performance liquid
chromatography یا HPLC) اثر عوامل مختلف و هم‌افزایی سویه‌ها مورد سنجش قرار گرفت. بر اساس نتایج، جاذب آلی بررسی شده می‌تواند مایکوتوکسین زیرالنون را جذب کرده و مقدار آن را در نمونه کاهش دهد. همچنین جاذب حرارت دیده و فعال در محیط کشت بهترین جذب را داشتند. بررسی ارزیابی هم‌افزایی سویه‌ها نیز کاهش بیشتر سم نسبت به هریک از سویه‌ها به تنهایی را نشان داد. بنابر این، جاذب آلی مورد مطالعه جهت کنترل و کاهش آلودگی مایکوتوکسین زیرالنون پیشنهاد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

In vitro evaluation of the effect of the organic adsorbent on the reduction of mycotoxin Zearalenone

نویسندگان [English]

  • Fatemeh Adami Ghamsari 1
  • Maryam Tajabadi Ebrahimi 2
  • Maryam Bagheri Varzaneh 3
  • Alireza Iranbakhsh 4
  • Abbas Akhavan Sepahi 5

1 PhD student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

3 Assistant ProfessorDepartment of Agriculture, Iranian Research Organization for Science and Technology, Tehran, Iran

4 ProfessorDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

5 Associate ProfessorDepartment of Microbiology, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Teheran, Iran

چکیده [English]

Zearalenone (ZEA) is one of the most important and abundant fungal toxins that causes contamination of food and feed of livestock and poultry as well as serious damages to humans and livestock. The present study investigated the effect of organic adsorbent containing four strains of native Lactobacillus and the yeast cell wall of Saccharomyces cerevisiae on the reduction of the ZEA mycotoxin. Using high-performance liquid chromatography (HPLC), the effects of different factors including incubation time, temperature, as well as synergism of the strains were measured. Based on the obtained results, the investigated organic adsorbent can adsorb the mycotoxin ZEA and reduce its amount in the sample. Also, the heated and culture medium activated adsorbents possessed the best absorption. Evaluation of synergistic of strains showed a greater reduction of the toxin than each strain alone. Therefore, the studied organic adsorbent is proposed to control and reduce mycotoxin ZEA contamination.

کلیدواژه‌ها [English]

  • Probiotic
  • Adsorption
  • Yeast cell wall
  • Toxins
  • Lactobacillus
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