نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار موسسه تحقیقات فنی و مهندسی کشاورزی- سازمان تحقیقات، آموزش و ترویج کشاورزی
2 استاد موسسه تحقیقات ف نی و مهندسی کشاورزی، سازمان تحق یقات، آموزش و ترویج کشاورزی
چکیده
سطح برگ نقش مهمی در تجزیه و تحلیل رشد و فتوسنتز دارد. به طور سنتی سطح برگ با استفاده از معادله رگرسیون، روش شمارش شبکه، روش توزین و پلانیمتری اندازهگیری میشود. در این مقاله یک الگوریتم ساده، سریع، غیرمخرب و دقیق برای اندازهگیری سطح برگ با استفاده از پردازش تصویر پیادهسازی شده است. تصویر از دوربین تلفن همراه به دست میآید و در فرمت JPEG ذخیره میشود. سپس در نرمافزار تدوین شده به فرمت Bitmap تبدیل میشود. تصویر تبدیل شده رنگی با استفاده از روش آستانه تقسیمبندی میشود. نویزهای تصویر در منطقه برگ و مقیاس با استفاده از تکنیک انتخاب رنگ منطقه حذف میگردد. تعداد پیکسلها در جسم مربع (مقیاس) و منطقه برگ محاسبه و سطح برگ با شمارش تعداد پیکسلها و با توجه به مقیاس، تعیین میشود. در این تحقیق، از 6 گیاه مختلف پهنبرگ، باریکبرگ، پنجهای و کشیده (انگور، آلو، گردو، انجیر، ذرت و نعناع) برای آزمون نرمافزار پیشنهادی استفاده شد. از هر گیاه مساحت 10 برگ مورد اندازهگیری قرار گرفت، استفاده از الگوریتم فوق در سطح اطمینان 99٪ با سایر روشهای اندازهگیری (پویشگر ثابت، ترسیم بر کاغذ و استفاده از ابزار مساحتسنجی مرسوم) تفاوت معنیداری نداشت.
کلیدواژهها
عنوان مقاله [English]
Development of software for measuring the surface area of the leaves and comparing with conventional methods
نویسندگان [English]
- Hooman Sharifnasab 1
- Fariborz Abbasi 2
1 Assistant Professor of Agricultural Technical and Engineering Research Institute - Agricultural Research, Education and Extension Organization
2 Professor at the Institute of Agricultural Research and Engineering, Agricultural Research, Education and Extension Organization
چکیده [English]
The leaf area plays an important role in growth analysis and photosynthesis. Traditionally, leaf area is measured using the regression equation, network counting method, weighing and planimeter method. In this paper, a simple, fast and accurate algorithm is implemented to measure the leaf area using image processing. The image is obtained from the mobile camera and is stored in JPEG format. Then it is converted into Bitmap format in the software. The converted color image is subdivided using the threshold method. Image noise is removed in the area of the leaf and scale using the region color picker technique. The number of pixels in the square object (scale) and the leaf area is calculated and the leaf area is determined by counting the number of pixels and according to the scale. In this research, six different leafy, leafy, clawed and pulled leaves (grapes, plums, walnuts, figs, corn and peppermint) were used and from each plant, the area of 10 leaves was measured. Using the proposed algorithm at 99% confidence level, there were no significant differences with other measurement methods (constant scanner, drawing on paper and using conventional area measurements).
کلیدواژهها [English]
- Camera
- mobile phone
- non-destructive
- scanner
The involvement of Mig1 from Xanthophyllomyces dendrorhous in catabolic repression: an active
mechanism contributing to the regulation of carotenoid production. PloS one, 11(9): e0162838-62.
Basha, N. S., Rekha R., Komala M., and Ruby S. (2009). Production of extracellular anti-leukaemic enzyme
lasparaginase from marine actinomycetes by solidstate and submerged fermentation: Purification and
characterisation. Tropical Journal of Pharmaceutical Research. 8(4): 353-360.
Benzie, I. F., and Szeto, Y. T. (1999). Total antioxidant capacity of teas by the ferric reducing/antioxidant
power assay. Journal of agricultural and food chemistry, 47(2): 633-636.
Britton, G., and Khachik, F. (2009). Carotenoids in food. Carotenoids, 45-66.
Cabral, M. M. S., Cence, K., Zeni, J., Tsai, S. M., Durrer, A., Foltran, L. L., ... and Treichel, H. (2011).
Carotenoids production from a newly isolated Sporidiobolus pararoseus strain by submerged
fermentation. European Food Research and Technology, 233(1), 159-166.
Chisté, R.C., Freitas, M., Mercadante, A.Z., and Fernandes, E. (2014). Carotenoids inhibit lipid peroxidation
and hemoglobin oxidation, but not the depletion of glutathione induced by ROS in human erythrocytes.
Life Sciences 99, 52-60.
da Costa Cardoso, L.A., Kanno, K.Y.F., and Karp, S.G. (2017). Microbial production of carotenoids A review.
African Journal of Biotechnology 16, 139-146.
Delgado-Vargas, F., Jiménez, A. R., and Paredes-López, O. (2000). Natural pigments: carotenoids,
anthocyanins, and betalains—characteristics, biosynthesis, processing, and stability. Critical reviews in
food science and nutrition, 40(3): 173-289.
El-Banna, A. A., El-Razek, A. M. A., and El-Mahdy, A. R. (2012). Some factors affecting the production of
carotenoids by Rhodotorula glutinis var. glutinis. Food and Nutrition Sciences, 3(01): 64-71.
Ferreira ICFR, Barros L, Soares ME, et al. (2007) Antioxidant activity and phenolic contents of Olea europaea
L. leaves sprayed with different copper formulations. Food Chemistry 103: 188-195
Gabani, P., and Singh, O. V. (2013). Radiation-resistant extremophiles and their potential in biotechnology and
therapeutics. Applied microbiology and biotechnology, 97(3): 993-1004.
Gmoser, R., Ferreira, J., Lundin, M., Taherzadeh, M., and Lennartsson, P. (2018). Pigment production by the
edible filamentous fungus Neurospora intermedia. Fermentation, 4(1): 11-26.
Ito, H., Watanabe, H., Takehisa, M., and Iizuka, H. (1983). Isolation and identification of radiation-resistant
cocci belonging to the genus Deinococcus from sewage sludges and animal feeds. Agricultural and
biological chemistry, 47(6): 1239-1247.
Khodaiyan, F., Razavi, S.H., and Mousavi, S.M. (2008). Optimization of canthaxanthin production by Dietzia
natronolimnaea HS-1 from cheese whey using statistical experimental methods. Biochemical Engineering
Journal. 40, 415-422.
Kanzy, H. M., Nasr, N. F., El-Shaz, H. A. M., and Barakat, O. S. (2015). Optimization of carotenoids
production by yeast strains of Rhodotorula using salted cheese whey. International Journal of Current
Microbiology and Applied Sciences, 4(1): 456-469.
Kirti, K., Amita, S., Priti, S., Mukesh Kumar, A., and Jyoti, S. (2014). Colorful world of microbes: carotenoids
and their applications. Advances in Biology, 2014.
Manimala MR, and Murugesan R. (2014). In vitro antioxidant and antimicrobial activity of carotenoid pigment
extracted from Sporobolomyces sp. isolated from natural source. Journal of Applied and Natural Science.
6(2): 649-653.
Mitra, R., Samanta, A. K., Chaudhuri, S., and Dutta, D. (2015). Growth kinetics and carotenoid production of
a newly isolated bacterium on glucose as a carbon source during batch fermentation. 5th Annual
International Conference on Advances in Biotechnology (BioTech 2015), 13-15 March, Kanpur, India.
Mohana, D., Thippeswamy, S., andAbhishe, R. (2013). Antioxidant, antibacterial, and ultraviolet-protective
properties of carotenoids isolated from Micrococcus spp. Radiation Protection and Environment, 36(4):
168-174.
NasriNasrabadi, M. R., and Razavi, S. H. (2010). Enhancement of canthaxanthin production from Dietzia
natronolimnaea HS-1 in a fed-batch process using trace elements and statistical methods. Brazilian Journal
of Chemical Engineering, 27(4): 517-529.
Oliver, J., and Palou, A. (2000). Chromatographic determination of carotenoids in foods. Journal of
chromatography A, 881(1-2): 543-555.
Rajeswari, P., Jose, P. A., Amiya, R., &Jebakumar, S. R. D. (2015). Characterization of saltern based
Streptomyces sp. and statistical media optimization for its improved antibacterial activity. Frontiers in
Microbiology, 5, 753.
Rezaeeyan, Z., Safarpour, A., Amoozegar, M. A., Babavalian, H., Tebyanian, H., andShakeri, F. (2017). High
carotenoid production by a halotolerant bacterium, Kocuria sp. strain QWT-12 and anticancer activity of
its carotenoid. Excli journal, 16: 840-851.
Sandmann, G. (2014). Carotenoids of biotechnological importance. Pages 449-467 in Biotechnology of
Isoprenoids, Springer.
Sasidharan P, Raja R, Karthik C, Ranandkumar S, and Indra Arulselvi P. (2013) Isolation and characterization
of yellow pigment producing Exiguobacterium sps. Journal of Biochemical Technology 4(4): 632-635.
Shariati, S., Zare, D., &Mirdamadi, S. (2019). Screening of carbon and nitrogen sources using mixture analysis
designs for carotenoid production by Blakesleatrispora. Food science and biotechnology, 28(2), 469-479.
Sharma, O. P., and Bhat, T. K. (2009). DPPH antioxidant assay revisited. Food chemistry, 113(4): 1202-1205.
Siso, M. G. (1996). The biotechnological utilization of cheese whey: a review. Bioresource Technology, 57(1),
1-11.
Squillaci, G., Parrella, R., Carbone, V., Minasi, P., La Cara, F., and Morana, A. (2017). Carotenoids from the
extreme halophilic archaeon Haloterrigena turkmenica: Identification and antioxidant activity.
Extremophiles, 21(5): 933-945.
Tian, B., Sun, Z., Shen, S., Wang, H., Jiao, J., Wang, L., and Hua, Y. (2009). Effects of carotenoids from
Deinococcus radiodurans on protein oxidation. Letters in applied microbiology, 49(6): 689-694.
Tsai S-Y, Huang S-J and Mau J-L (2006) Antioxidant properties of hot water extracts from
Agrocybecylindracea. Food Chemistry 98: 670-677.
Zhao, J. H., Bai, F. Y., Guo, L. D., and Jia, J. H. (2002). Rhodotorula pinicola sp. nov., a basidiomycetous
yeast species isolated from xylem of pine twigs. FEMS yeast research, 2(2): 159-163.
Zamanian, S. N., and Etemadifar, Z. (2017). Radical scavengering of pigments from novel strains of Dietzia
schimae and Microbacteriumest eraromaticum. Progress in Biological Sciences, 6(2): 159-170.