نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترا .گروه علوم پایه، دانشکده داروسازی و علوم دارویی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه علوم پایه، دانشکده داروسازی و علوم دارویی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران

3 استاد موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی ، تهران ، ایران

10.22051/jab.2020.29585.1342

چکیده

در این پژوهش اثر داروی سروتونرژیک اس‌سیتالوپرام بر سیستم تولیدمثل پس از القای دوپامین با تزریق بروموکریپتین در ماهی گورامی سه خال ماده بررسی گردید. تعداد 90 قطعه ماهی گورامی سه خال ماده با میانگین وزنی 1±3 گرم از مرکز پرورش ماهیان خانماهی دماوند تهیه شد. ماهی‌ها در شش گروه 15تایی شامل کنترل دست‌نخورده، حلال(اتانولº70) ، بروموکریپتین و سه گروه تیمار که دریافت کننده ی بروموکریپتین و اس‌سیتالوپرام در سه دوز 1، 5 و 10 میلی‌گرم بر کیلوگرم وزن بدن قرار گرفتند. تزریق به صورت عضلانی(IM) و یک روز در میان صورت گرفت. سپس ماهی ها تشریح شده و بافت تخمدان جهت بررسی با میکروسکوپ نوری و الکترونی جداسازی شد. هورمون های استروئیدی با کیت مخصوص اندازه گیری شد. با توجه به نتایج به دست آمده از تیمار دوز دوم داروی اس‌سیتالوپرام(5 میلی‌گرم بر کیلوگرم وزن بدن ماهی) به همراه بروموکریپتین، که مرحله ی غالب ویتلوژنز بود و شاخص گنادی و هورمون‌های استروئیدی در بیشترین مقدار خود است می توان نتیجه گرفت که امکان حضور گیرنده ی سروتونین در سطوح پایینی محور HPG وجود دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Escitalopram on sex steroids and oocyte ultrastructure in three spot Gourami after increasing dopamine level by Bromocriptine injection

نویسندگان [English]

  • Mona Sadat Hosseini jebeli 1
  • Tahereh Naji 2
  • Homayoun Hosseinzadeh Sahafi 3

1 PHD student, Department of Basic sciences, Faculty of Pharmacy & Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University,Tehran,Iran

2 Basic Sciences Department, Faculty of Pharmacy & Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

3 Professor Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran

چکیده [English]

In this study, we aimed to investigate the effect of serotonin on the lower levels of HPG by blocking the upper HPG axis.90 pieces of three spot Gourami fish with an average weight of 3±1 gram were bought from the Khanmahi fish breeding center. The fish were divided into 6 groups. Intact Control group, solvent (Ethanol 70º) group, bromocriptine control and 3 treatment group received bromocriptine and escitalopram in three doses of 1,5 and 10 milligram per kilogram body weight. The injection was given intramuscular (IM) every other day. The fish were dissected and ovarian tissues dissected for light and electron microscope. After tissue fluids preparation, steroid hormones were measured with a special kit. Based on the results of the second dose of escitalopram and bromocriptine which was the predominant stage of vitellogenesis and gonadal index, it can be concluded that serotonin receptor may be present at the lower levels of the HPG axis.

کلیدواژه‌ها [English]

  • Dopamine
  • Gonadotropin
  • Ovary
  • Serotonin
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