نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار، گروه تولیدات گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه گنبدکاووس، گنبدکاووس
2 کارشناسی ارشد رشته علوم علفهای هرز، دانشکده کشاورزی و منابع طبیعی دانشگاه گنبدکاووس، گنبدکاووس
3 دانشیار، گروه تولیدات گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه گنبدکاووس، گنبدکاووس
4 مربی، گروه زیستشناسی دانشکده علوم پایه دانشگاه گنبدکاووس، گنبدکاووس
چکیده
هدف از تحقیق حاضر بررسی ویژگی‌های مورفولوژیکی و بیوشیمیایی گیاهچه‌های برنج رقم دمسیاه به غلظتهای مختلف (0، 024/0، 048/0، 076/0 و 1/0 میلی مولار) ترکیبات فنلی استخراج شده حاصل از علفهرز سوروف (Echino crus-galli (L.) Beauv) بود. آزمایش در قالب طرح کاملاً تصادفی در سه تکرار در محیط کشت هیدروپونیک انجام شد. از متانول برای استخراج ترکیبات فنلی از علفهرز سوروف استفاده شد. در آزمایش دیگری، غلظتهای مختلف فنل سنتزی (هیدروکسی‌بنزن) نظیر 0، 514/1، 054/3، 569/4 و 136/6 میلیمولار نیز بر این رقم اعمال گردید. مطابق نتایج، تاثیر غلظتهای مختلف ترکیبات فنلی سوروف بر صفات طول ریشه، عرض بزرگ‌ترین برگ، درصد رطوبت نسبی برگ، محتوای پروتئین و فنل‌کل برنج در سطح احتمال 5 درصد معنی‌دار بود، به‌گونه‌ای که با افزایش غلظتهای مختلف ترکیبات فنلی علفهرز سوروف، میزان تاثیر منفی بر این صفات در مقایسه با شاهد افزایش یافت. بیشترین کاهش این صفات مربوط به غلظت 1/0 میلیمولار از ترکیبات فنلی بوده است، اگرچه اختلاف بین این تیمار با برخی از تیمارها معنی‌داری نبود. بر اساس نتایج، اثر کاهشی غلظتهای مختلف عصاره فنل سنتزی به‌ویژه در غلظتهای بالا علاوه بر صفات مورد بررسی بر سطح برگ در بوته، محتوای رنگیزه‌های کلروفیلی a، b، کل برنج، بیش از تیمارهای طبیعی آن در سوروف بوده است. با توجه به مشاهده تاثیر منفی ترکیبات فنلی سوروف بر گیاهچه‌های برنج رقم دمسیاه و هم‌چنین وجود مقادیر قابل ملاحظه‌ای از گروه‌های آروماتیک مشتقات بنزن نظیر ترکیبات فنلی و آلیفاتیک با زنجیره‌ای از کربن در ساختار شیمیایی اولیه اکثر علف‌کش‌های سنتزی، پیشنهاد به کاشت ارقام مقاوم و
کلیدواژه‌ها
عنوان مقاله [English]
Comparision of synthesis phenolic compounds and extracted from barnyard grass weed (Echinochloa crus-galli (L.) Beauv) on morphological and biochemical characteristics of rice seedlings- Domsia cultivar
نویسندگان [English]
- Ebrahim Gholamalipour Alamdari 1
- Amir Ghorbani 2
- Zeinab Avarseji 3
- Meisam habibi 4
- abdollah Ataei 2
1 Assistant Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous
2 M.Sc. in Weed Science, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous
3 Associate Professor, Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad-e Kavous University, Gonbad-e Kavous
4 Instructor, Department of Biology, Faculty of Basic Sciences, Gonbad Kavous University, Gonbad Kavous
چکیده [English]
The purpose of this study was eavaluation of morphological and biochemical haracteristics of rice seedlings–Domsia cultivar to various concentrations (0, 0.024, 0.048, 0.076 and 0.1 mM) of extracted phenolic compounds of barnyard grass (Echino crus-galli (L.) Beauv). The experiment was performed based on a completely randomized design in three replications in hydroponic culture. Methanol was used to extraction of the phenolic compounds. In other experiment, different concentrations of hydroxyl benzene synthesis (0, 1.514, 3.054, 4.569 and 6.136 mM) also was applied on this cultivar. According to the results, effects of different concentrations of phenolic compounds of barnyard grass on charactristics of root length, width of largest leaf, relative water content of leaf, protein and total phenols of rice seedlings were significant at 5% probability level. So that, the extent of negative impact on these traits were increased with increasing concentrations of phenolic compouds of barntyard grass in comparison with control. The greatest reduction of these traits was related to 0.1 Mm of phenolic compounds concentration, although there was no significant difference between this treatment and some treatments. According to the results, significant reduction effect of synthetic phenolic compounds concentrations on studied traits in addition to leaf area in plant and content of chlorophyll a, b and total especially at higher concentrations was more than treatments of its natral concentrations in barnyard grass. Due to the observed negative impact of phenolic compounds of barntyardgrass on rice seedlings–Domsia cultivar and also exstance of considerable amount of aromatic groups of benzene derivatives such
کلیدواژه‌ها [English]
- Clorophyll pigment
- Leaf area
- Rice–Domsia cultivar
- Synthetic phenol extract
- Various concentrations
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