نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد . گروه زیست شناسی سلولی مولکولی و میکروبیولوژی، دانشکده علوم و فناوریهای زیستی، دانشگاه اصفهان، اصفهان، ایران

2 دانشیار .گروه زیست شناسی سلولی مولکولی و میکروبیولوژی، دانشکده علوم و فناوریهای زیستی، دانشگاه اصفهان، اصفهان، ایران

3 استادیار گروه زیست شناسی، دانشکده علوم، دانشگاه گنبدکاووس، گلستان، ایران

10.22051/jab.2021.31100.1366

چکیده

در مطالعه حاضر، اثر منابع کربنی و نیتروژنی مختلف بر زیست ‌توده و رنگدانه کاروتنوئیدی Deinococcus radiodurans به‌عنوان یکی از مقاوم‌ترین میکروارگانیسم‌ها به اشعه، با استفاده از رهیافت یک فاکتور در یک‌زمان مورد بررسی قرار گرفت. همچنین خصوصیات آنتی‌اکسیدانی، سمیت سلولی و فعالیت ضدمیکروبی رنگدانه نیز بررسی شدند. رنگدانه کاروتنوئیدی دارای نصف حداکثر غلظت مؤثر (Half-maximal effective concentration =EC50) برابر با μg/mL 19/20 و mg/mL 28/3 به ترتیب در تست‌های قدرت احیاکنندگی یون‌های آهن فریک و به دام اندازی رادیکال‌های آزاد بود. حداکثر میزان تولید کاروتنوئید در حضور g/L 1 گلوکز و g/L 1 عصاره مخمر بترتیب معادل mg/L 34 و mg/L 28 به دست آمد. بر اساس رهیافت یک فاکتور در یک زمان، شرایط بهینه تولید کاروتنوئید به میزان mg/L37 رنگدانه در محیط کشت حاوی منبع کربنی g/L 1 گلوکز و منبع نیتروژنی g/L 1 عصاره مخمر به‌دست آمد. همچنین این رنگدانه اثر سمی یا مهاری بر روی سلول‌های فیبروبلاستی انسان و بر روی دو باکتری Staphylococcus epidermidis و Escherichia coli نداشت.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of Biomass and Total Carotenoids Content of Deinococcus radiodurans R1 in Response to Different Carbon and Nitrogen Sources

نویسندگان [English]

  • Atefeh Salehi Bakhtiyari 1
  • Zahra Etemadifar 2
  • Matia Sadat Borhani 3

1 MSC . Department of Molecular Cell Biology and Microbiology, Faculty of Science and Biotechnology, University of Isfahan, Isfahan, Iran

2 Associate Professor, Department of Molecular Cell Biology and Microbiology, Faculty of Biological Sciences and Technologies, University of Isfahan, Isfahan, Iran

3 Assistant Professor, Department of Biology, Faculty of Science, Gonbad Kavous University, Golestan, Iran

چکیده [English]

In the present study, the effect of different carbon and nitrogen sources on the microbial biomass and carotenoid production of Deinococcus radiodurans, one of the most radiation-resistant microorganisms, was evaluated using one-factor-at-a-time approach. The antioxidant, cytotoxicity and antibacterial properties of the pigment were also evaluated. The carotenoid pigment had EC50 (Half maximal effective concentration) of 20.19 μg/mL and EC50 = 3.28 mg/mL in the ferric reducing antioxidant power and free radical scavenging assay, respectively. The maximum amount of carotenoid pigment was achieved in the presence of 1g/L glucose (34 mg/L) and 1 g/L yeast extract (28 mg/L). Based on the approach of one factor at a time, the optimal conditions for carotenoid production were obtained as 37 mg/L pigment in culture medium containing 1 g/L glucose carbon source and 1 g/L nitrogen source of yeast extract. Besides, the pigment had no toxic or inhibitory effects on the human fibroblast cell line and two bacteria Staphylococcus epidermidis and Escherichia coli.

کلیدواژه‌ها [English]

  • Antioxidant
  • Optimization
  • pigment
  • Radiophile
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