نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکترادانشکده علوم زیستی، دانشگاه آزاد اسلامی، واحد تهران شمال
2 دانشیار دانشگاه آزاد اسلامی واحد تهران شمال
3 دانشیار دانشکده علوم زیستی، دانشگاه آزاد اسلامی واحد تهران-شمال
4 دانشیار دانشکده علوم ، دانشگاه آزاد اسلامی واحد علوم و تحقیقات
چکیده
گل‌حسرت Colchicum speciosum گیاهی دارویی غنی از کلشیسین و ترکیبات فنلی است. در این مطالعه تاثیر غلظت های مختلف کودهای ازت و نانو ازت بر محتوای ترکیبات فنلی وآلکالوئید کلشیسین در بنه گیاهان گل‌حسرت از سه منطقه در استان مازندران (فیل‌بند، کلرد و سنگ‌درکا) بررسی شد. آزمایش با طرح بلوک های تصادفی برای پنج تیمار شامل تیمار شاهد، دو سطح کود ازت (mg/l1/1 و2/2)، دو سطح کود نانو ازت (mg/l5/1 و 3) برای گیاهان سه رویشگاه اجرا شد. سه ماه پس از شروع تیمار، ترکیبات فنلی و آلکالوئید کلشیسین بنه استخراج شد. محتوای ترکیبات فنلی با روش اسپکتروفتومتری و کلشیسین با HPLC سنجش شدند در گیاهان رویشگاه فیل‌بند همه تیمارهای ازت و نانو ازت موجب افزایش معنی‌دار (P≤0.05 ) محتوای فنل کل بنه شد. استفاده از کودهای ازت و نانو ازت موجب کاهش معنی‌دار محتوای فلاونوئید کل در بنه گیاهان رویشگاه‌های مورد مطالعه شد. کود ازت در همه جمعیت‌ها موجب افزایش معنی‌دار محتوای آنتوسیانین کل بنه شد، در حالی‌که تیمار با کود نانو ازت تاثیر معنی‌دار بر میانگین محتوای آنتوسیانین کل بنه نداشت. مقایسه نتایج میانگین محتوای کلشیسین بنه در تیمارهای مختلف نشان داد که کودهای ازت موجب کاهش و کودهای نانو ازت سبب افزایش محتوای کلشیسین بنه می‌شوند. نتایج حاضر نشان داد محتوای متابولیتهای مورد مطالعه در بنه رویشگاه‌های مختلف با یکدیگر تفاوت دارند و تاثیر کودهای ازت و نانو ازت برتغییر محتوای این متابولیت‌ها یکسان نیست.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of nitrogen and nano nitrogen fertilizers on corm colchicine and phenolic compounds of Colchicum speciosum from three regions of Mazandaran
نویسندگان [English]
- Masoud Babaie 1
- Maryam Peyvandi 2
- Hossein Abbaspour 3
- Zahra Noormohammadi 4
- Sedighe Arbabian 3
1 MSCFaculty of Biological Science, North Tehran Branch, Islamic Azad University
2 Associate Professor,Islamic Azad University, Tehran North Branch
3 Associate Professor,Faculty of Biological Science, Islamic Azad University, Tehran North Branch
4 Associate Professor,Science and Research Branch, Islamic Azad University
چکیده [English]
Colchicum speciosum is a medicinal plant rich in colchicine and phenolic compounds. In this study, the effect of different concentrations of nitrogen and nano-nitrogen fertilizers were investigated on phenols and colchicine accumulation in the corm of C.speciosum from three regions in Mazandaran province (Philband, Kelerd and Sangdarka). Five fertilizer treatments were performed. The results showed that all nitrogen and nano-nitrogen treatments caused a significant increase in corm phenol in the plants of the Philband region. Comparison of the mean flavonoids of plants in three regions in different treatments showed that the use of nitrogen and nano-nitrogen fertilizers has significantly reduced flavonoids in plant corms of all studied plants. The results showed that nitrogen fertilizer (1.1 mg / l) caused a significant increase in corm anthocyanin in all three populations. Nitrogen fertilizer with higher concentration also increased anthocyanin to some extent, while nano-nitrogen at different concentrations did not have a significant effect on the amount of anthocyanin in corm. Comparison of corm colchicine in nitrogen and nano-nitrogen treatments with control showed that nitrogen fertilizers in both concentrations decreased and nano-nitrogen fertilizers increased the amount of corm colchicine. The present results showed that the response of plants collected from the three regions to the different fertilizers was not the same.
کلیدواژه‌ها [English]
- Colchicum speciosum
- Nano-Nitrogen
- Anthocyanin
- Flavonoid
- Colchicine
- Corm
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