نوع مقاله : مقاله پژوهشی

نویسندگان

استادیار ، گروه شیمی ، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان،تبریز

چکیده

قارچ­های طاقچه­ای یکی از مهمترین قارچ­های ماکروسکوپی هستند که نقش بسزایی را در اکوسیستم ایفا می­کنند. در این تحقیق، ابتداویژگی­های مورفولوژیکی قارچ Cerrena unicolorمطالعه شد و سپس، تاثیر حلال­های مختلف (n-هگزان، دی­کلرومتان، اتیل­استات و متانول) در استخراج ترکیبات فنولی، خاصیت­ آنتی­­اکسیدانی و سمیت­سلولی مورد بررسی قرار گرفت. میزان فنول و فلاونوئید کل، آنالیز فنولیک­اسیدها، خاصیت آنتی­اسیدانی و فعالیت سمیت­سلولی بر علیه رده سلولی سرطانی سینه (MCF-7) به ترتیب با روش­های اسپتروفتومتری، کروماتوگرافی مایع با کارایی بالا (HPLC مهار رادیکال آزاد (DPPH)، و آزمون رنگ سنجی MTT مورد ارزیابی قرار گرفتند. نتایج نشان داد که بازیدیوکارپشکل نیم­دایره و موج­دار داشته و سطح بالایی آن پوشیده از کرک­­های سفید تا قهوه­ای روشن بود. بازیدیوم­ها به طول 25 میکرومتر، چماغی شکل و حامل چهار استریگما با بازیدیوسپورهای بیضوی بودند. همچنین، نتایج نشان داد که در مقایسه با دیگر عصاره­ها، عصاره متانولی بیشترین میزان فنول و فلاونوئید کل را داشت. بعلاوه، بنزوئیک­اسید، پروتوکاتکوئیک اسید، پاراکوماریک­اسید، گالیک­اسید و سینامیک­اسید عمده­ترین فنولیک­اسیدهای موجود در عصاره متانولی و اتیل­استاتی بودند. علاوه بر این، عصاره متانولی بیشترین خاصیت آنتی­اکسیدانی در مهار رادیکال­های آزاد DPPH و عصاره­های متانولی و اتیل­استاتی بیشترین خاصیت سمیت­سلولی بر علیه رده­سلولی MCF-7 را داشتند. بنابراین، عصاره متانولی بدلیل داشتن ترکیبات فنولی، می­تواند بعنوان منبع جدید آنتی­اکسیدان طبیعی با خاصیت سمیت­سلولی مطرح باشد. 

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of morphological, phytochemical and biological activity of bracket fungi Cerrena unicolor collected from Arasbaran forests

نویسندگان [English]

  • Saeed Mollaei
  • Mostafa Ebadi

Assistant Professor, Department of Chemistry, Faculty of Basic Sciences, Azerbaijan Shahid Madani University, Tabriz

چکیده [English]

Bracket fungi are the most important macroscopic fungi that play a main role in the ecosystem. In this study, the morphological characters of Cerrena unicolor were evaluated and then, the effect of different solvents (n-Hexan, Dichloromethane, Ethyl acetate, and Methanol) on the extraction of phenolic compounds, antioxidant properties, and cytotoxic activities of the extracts were investigated. Total phenol and flavonoid content, phenolic acid analysis, antioxidant properties, and cytotoxic activities on MCF-7 cells were evaluated by spectrophotometry, HPLC, DPPH, and MTT methods, respectively. The obtained results showed the basidiocarp was semicircular and wavy, and the upper surface was covered with small white to grayish brown hairs. Basidia were 25 µm in length, calvate and including 4 sterigmata with ellipsoid basidiospores. The results also indicated that the Methanolic extract had the highest total phenol and flavonoid contents compared with other extracts. Moreover,Benzoic acid, Protocatechuic acid, Gallic acid, and Cinnamic acid were the main phenolic acids of the Methanolic and Ethyl acetate extracts. In addition, the Methanolic extract showed the highest antioxidant activity in inhibiting DPPH radicals, while the Methanolic and Ethyl acetate extracts indicated the highest cytotoxic activity on MCF-7 cells. Therefore the Methanolic extract due to phenolic compounds could be proposed as a new sourceofnatural antioxidants with cytotoxic activity.

کلیدواژه‌ها [English]

  • Antioxidant
  • Basidiocarp
  • Basidiomycetes
  • Cytotoxic activity
  • Methanolic extract
  • Phenolic compounds
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