نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه الزهرا س

2 گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه الزهراس

چکیده

مطالعات نشان داده است که گلسنگ‌ها در اکوسیستم‌های مختلف حضور فعالی داشته و متابولیت‌های متنوعی تولید می‌کنند که تاثیرات زیستی مختلفی از جمله اثرات ضدباکتری، ضدقارچی، ضدویروسی، ضدالتهابی، ضدتب، ضددرد و سمیت سلولی دارند. در این مطالعه، اثر ترکیبات ضد میکروبی گلسنگ‌ها بررسی شد. برای این منظور، ابتدا چند نمونه از گلسنگ‌های مناطق جنگلی اطراف شهر بابل جمع آوری و به کمک مشاهدات ماکروسکوپی و میکروسکوپی، آزمون های بیوشیمیایی و مولکولی، شناسایی شدند. سپس عصاره اتانولی گلسنگ‌ها تهیه شد و اثر ضد باکتریایی و ضد قارچی آن‌ها به طریق انتشار در آگار و تعیین حداقل غلظت ممانعت از رشد (MIC) علیه پنج باکتری،Escherichia coli ,Staphylococcus aureus ,Pseudomonas aeruginosa ,Bacillus cereus ,Xanthomonas campestris و سه مخمر,Candida albicans ,Candida tropicalis Candida glabrata بررسی شد. نتایج نشان داد که عصاره گلسنگ‌هایLeptogium saturninum, Ramalina peruviana, Punctelia borreri علیهX. campestris وS. aureus موثر بودند. این در حالی است که در مورد E. coli وB. cereus تنها عصاره گلسنگ R. peruviana موثر بود و باکتری گرم منفی P.aeruginosa نیز نسبت به همه عصاره‌ها مقاومت نشان داد. همچنین اندازه‌گیری قطر هاله‌ی عدم رشد و نتایج MIC نشان داد که عصاره Leptogium saturninumو Ramalina peruviana روی هر سه مخمر موثر بودند. این در حالی است که عصاره گلسنگ P. borreri تنها بر C. albicans موثر بود. با توجه به تولید ترکیبات ضدمیکروبی طبیعی در گلسنگ‌ها، استفاده از آن‌ها در صنایع دارویی توصیه می‌شود. تحقیقات بیشتری لازم است تا اثر ضدمیکروبی گلسنگ ها بر طیف وسیعی از میکروارگانیسم‌های بیماری‌زا و نیز محیطی بررسی گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Antimicrobial effects of Leptogium saturninum, Ramalina peruviana and Punctelia borreri

نویسندگان [English]

  • Faezeh Hamzekhani 1
  • Parisa Mohammadi 1
  • Azra Saboora 2

1 Department of Microbiology, Faculty of Biological Sciences, Alzahra University

2 Department of plant Science, Faculty of Biological Sciences, Alzahra University

چکیده [English]

Lichens produce high diversity of metabolites and they have different biological activities including antibacterial, antifungal, antiviral, anti-inflammatory and the cytotoxic effects. In this study, the impact of antimicrobial substances of lichens were studied. For this purpose, the lichens of northern forests around Babol city was collected and via the morphological and microscopic characteristics, biochemical and molecular methods were identified. Then, ethanol extracts of lichens were prepared. Antimicrobial effects of extracts was assayed using the diffusion agar method and the determination of minimum inhibitory concentration against five bacteria, Bacillus cereus, Xanthomonas campestris, Escherichia coli, Staphylococus aureus and Pseudomonas aeruginosa. The studies showed that the extract of Leptogium saturninum, Ramalina peruviana, Punctelia borreri were effective against X. campestris، S. aureus. However, in the case of Bacillus cereus and E.coli only the extract of R. peruviana was effective. Furthermore, measuring the diameter of zone inhibition and MIC against three clinical Candida albicans, Candida tropicalis, and Candida glabrat showed that the extracts of Leptogium saturninum, Ramalina peruviana were effective on all the strains. However the extract of Punktelia subrodecta was effective only on C.albicans. The substance of lichens, which assayed by biochemical tests showed that due to the presence of phenolic compounds, lichens show high antimicrobial properties. It should be carried out the antimicrobial experiments on a wide range of environmental and pathogenic microorganisms.

کلیدواژه‌ها [English]

  • Antibacterial
  • Antifungal
  • Lichen
  • MIC

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