نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی زیست شناسی علوم سلولی و مولکولی، دانشگاه تهران (پژوهشگر مرکز تحقیقات بیماری های گوارش و کبد مرکز آموزشی و درمانی رازی، رشت، ایران)

2 دانشیار، پردیس علوم،دانشکده زیست شناسی، بخش فناوری های زیستی، دانشگاه تهران،ایران

3 استادیار، پردیس علوم، دانشکده زیست شناسی، بخش فناوری های زیستی، دانشگاه تهران،ایران

چکیده

میکروبیوتای نوزاد تغذیه کننده از شیر مادر عمدتا شامل دو جنس بیفیدوباکتریوم و لاکتوباسیلوس است. زایمان طبیعی در مقایسه با سزارین در استقرار باکتری های مفید و تکوین مناسب سیستم ایمنی و دستگاه گوارش نوزاد نقش به سزایی دارد. هدف از این مطالعه مقایسه ی تاثیر میکروبیوتای مدفوع نوزاد چهار ماهه متولد شده با زایمان طبیعی تغذیه کننده از شیر مادر بدون سابقه مصرف آنتی بیوتیک و بیماری های گوارشی بر بقای زیستی دو نوع سلول متفاوت از نظر ماهیت رشد شامل سلولهای سرطانی روده (Caco-2) و سلولهای بنیادی سوماتیکی نامحدود (USSC) می باشد. استخراج میکروبیوتای مدفوعی با حلال اتیل استات انجام شد. پس از حذف حلال، حاصل استخراج در حجم کمی از DMSO حل شد. اثر غلظت های 2/5 و 5 میکروگرم/ میلی لیتر از میکروبیوتا بر سلول ها پس از 72 ساعت انکوباسیون با آزمون MTT سنجیده شد. نتایج نشان داد که بقای زیستی USSC ها با افزایش دوز افزایش یافت. در حالیکه در همین شرایط ، کاهش بقای زیستی سلول های Caco-2 مشاهده شد.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of the effect of infant fecal microbiota on the cell viability of colon cancer cells and Unrestricted Somatic Stem Cells

نویسندگان [English]

  • Sara Mavaddati 1
  • Javad Hamedi 2
  • Bahman Zeynali 3

1 Bachelor of Science in Cell and Molecular Biology, University of Tehran

2 Associate Professor, College of Science, School of Biology, Department of biotechnology, University of Tehran,Iran

3 Assistant Professor, College of Science, School of Biology, Department of biotechnology, University of Tehran,Iran

چکیده [English]

The microbiota of breast-fed infants are mainly consists of Bifidobacterium and Lactobacillus. Normal vaginal delivery has an important role in the colonization of beneficial bacteria and proper development of the immune system and gastrointestinal tract in comparison with infants born through cesarean section. In this study we examined the effect of microbiota of a 4-month-old breastfeeding infant born with normal vaginal delivery, without history of taking antibiotics and gastrointestinal disorders on the cell viability of two types of cells with different growth features including colon cancer cells (Caco-2) and Unrestricted Somatic Stem Cells (USSCs). After fecal sampling, the microbiota was extracted by ethyl acetate. After removing the solvent, the result of extraction was solved in a few volume of DMSO. The effects at concentrations of 2.5 and 5 µg/ ml on the following cells were determined after 72 hours of incubation using MTT assay. The results showed that the cell viability of USSCs was significantly increased as the concentration elevated. While at the same conditions, the viability of Caco-2 cells was decreased at the higher concentration significantly.

کلیدواژه‌ها [English]

  • Cell viability
  • Caco�2
  • Microbiota
  • USSCs
 
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