نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیارگروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاداسلامی، واحدقم، قم، ایران

2 استاد گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران

3 دانشجوی دکتری گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

  جنس Myosotis دارای پانزده گونه در ایران است و ویژگی تشخیصی آن میوه صاف، سیاه و براق می‌باشد. به منظور نشان دادن موقعیت فیلوژنتیکی Myosotis، 14گونه از آن به همراه گونه‌هایی ازقبیله Cynoglosseae s. l. با استفاده از مارکرکلروپلاستیrpl32-trnL و همچنین توالی ترکیبی cpDNArpl32-trnLUAG-nrDNAITS، به دو روش پارسیمونی و بایسین مورد بررسی قرار گرفتند. نتایج، تک تباری جنس Myosotis را تایید نمود. همچنین نشان داده شد دو زیرجنس Myosotis و Strophiostoma، بخشه‌های Myosotis و Exarrhena، وگونه‌های یکساله تک تبار نیستند. درکلادوگرام حاصل از توالی کلروپلاستی rpl32-trnL تنها گروه‌های C، E وF تک تبارند، اما داده‌های ترکیبی تک تباری شش گروه A، B، C، D، E و F را نشان داد. گروه F با داشتن ویژگی‌های تشخیصی متعدد، گروهی است که بر اساس تمام توالی‌ها و روش‌های آنالیزی تک تبار بود. این گروه با چهار گونه‌ی M.asiatica، M.alpestris، M.suaveolens و M.lithospermifolia در فلور ایران، با نام Alpestris به عنوان گروهی مجزا و تک تبار معرفی می‌گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Molecular phylogeny and Alpestris group confirmation in Myosotis L. genus based on combined nuclear and chloroplast data

نویسندگان [English]

  • Maryam Khoshsokhan mozaffar 1
  • Shahrokh kazempour osaloo 2
  • Mahboubeh Sherafati 3

1 Assistant Professor, Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran

2 Professor, Department of Plant Biology, Faculty of Biological Sciences,Tarbiat Modares University, Tehran, Iran

3 PHD student, Department of Plant Biology, Faculty of Biological Sciences,Tarbiat Modares University, Tehran, Iran

چکیده [English]

Myosotis L. (Boraginaceae) consists of 15 genera in Iran and distinguished by small, black and smooth nutlets. In order to determination of Myosotis phylogenetic position in generic and subgeneric level, 14 species of Myosotis along with some Cynoglosseae species were used. Matrices consist of chloroplstic rpl32-trnlUAG and combined nrDNAITS - cpDNArpl32-trnlUAG sequences were analyzed by maximum parsimony and Bayesian Inference. Results confirmed the monophyly of the Myosotis genus. Also Strophiostoma and Myosotis subgenera, Myosotis and Exarrhena sections and Myosotis annual species are not monophyletic. Based on chloroplast rpl32-trnlUAG sequences, C, E and F groups are monophyletic. Combined nuclear and chloroplast data showed the monophyly of the six A, B, C, D, E and F groups. Fgroup with numerous diagnostic characters was monophyletic in all data. This group is introduced as a distinct and monophyletic group called “Alpestris group” in Iran, consists of four species, M.asiatica, M.alpestris, M. suaveolens and M. lithospermifolia.

کلیدواژه‌ها [English]

  • Alpestris group
  • Boraginaceae
  • cpDNArpl32�trnLUAG
  • Molecular Phylogeny
  • Myosotis
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