Mehdi Shahriarinour; Zeinab Ahmadi; Ghoalam Khayati; Maasoumeh Anvari
Abstract
In this survey, the strains of bacillus sp. isolated from alkali solid with ability to produce alkaline protease were screened and identified .Then the enzyme production was assayed in different production affecting factors. The best solid substrate was combination of wheat straw and potato wastes ...
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In this survey, the strains of bacillus sp. isolated from alkali solid with ability to produce alkaline protease were screened and identified .Then the enzyme production was assayed in different production affecting factors. The best solid substrate was combination of wheat straw and potato wastes .Also the best production incubation time, moisture content and co-carbon sources .were 96h, 250% and Glucose respectively. Ammonium sulfate was the best nitrogen source for enzyme production enhancement. The maximum enzyme yield in the upper optimum condition was 6800u/g.
Najmeh Soltan mohammadi; Gholamali Naderi; Narges Jafari dinani
Abstract
Introduction: Free radicals leading to oxidative stress are involved in chronic diseases as cancer, atherosclerosis, diabetes and also aging. Antioxidants are the best way to Combating free radicals and the plants offer a wide range of natural antioxidants. Objective: This study was performed to evaluate ...
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Introduction: Free radicals leading to oxidative stress are involved in chronic diseases as cancer, atherosclerosis, diabetes and also aging. Antioxidants are the best way to Combating free radicals and the plants offer a wide range of natural antioxidants. Objective: This study was performed to evaluate of antioxidant activity of extracts of Humulus lupulus , Cynara Scolymus and Kelussia odoratissima that were prepared with soaked and soxhelet method. Methods: After extraction, total phenolic and flavonoid contents and antioxidant activity were estimated. Antioxidant activity is assayed using phosphomolybdenum method and was expressed as the number of equivalents of ascorbic acid and Quercetin . Results: Based on the results, t he relative order of plants according to amount of flavonoid and polyphenol contents is Humulus lupulus - Cynara Scolymus - Kelussia odoratissima and for antioxidant activity is Humulus lupulus - Kelussia odoratissima - Cynara Scolymus. Also, the results show that in Cynara Scolymus and Kelussia odoratissima , soaked method is more effective than soxhelet method whereas in Humulus lupulus, soxhelet is more effective than soaked method. Conclusion: The results suggest that flavonoid and polyphenol contents aren’t the only factors influencing antioxidant activity. Also, for more appropriate extraction of antioxidant components, determination type of extraction method is dependent on type of plant.
Mohammadreza Hasandokht; Sajad Jafari
Abstract
Watercress (Nasturtium Officinale L.) is a perennial member of the Brassicaceae family. Previously, it has been revealed that oral administration of this plant has a significant effect on diabetic rats and they have also contained the lower level of serum triglyceride indicating the anti-diabetic ...
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Watercress (Nasturtium Officinale L.) is a perennial member of the Brassicaceae family. Previously, it has been revealed that oral administration of this plant has a significant effect on diabetic rats and they have also contained the lower level of serum triglyceride indicating the anti-diabetic properties of the plant. The present study was aimed to introduce the best watercress population in terms of the highest antioxidant activity and total phenolic and vitamin C content for application in relevant industries such as pharmaceutical. In this study total phenolic and vitamin C content of different watercress populations were determined and their antioxidant activity was studied using the DPPH method. According to the results, Fashand (Alborz province) and Meimeh (Ilam province) exhibited the highest and the lowest antioxidant activity with the IC50 values of 70.1 and 375 ( µ g/ml), respectively. The positive correlation observed in this study between the level of phenolic compounds and ascorbic acid and antioxidant activity of tested samples indicates that the use of DPPH free radicals is one appropriate method for evaluation of antioxidant activity of methanol extracts. Watercress has a high level of antioxidant activity and Fashand population may be considered as the population with the highest antioxidant activity among tested populations.
Moj Khaleghi; Mohammad Bokaiean; Saeedeh Saeedi
Abstract
Since today, the number of pathogenic bacteria that are resistant to common used antibiotics is increasing, therefore, for controlling and treatment of infections that are caused by resistant microbes; discovery of new therapeutic agents is a necessary requirement. The aim of this study was assessment ...
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Since today, the number of pathogenic bacteria that are resistant to common used antibiotics is increasing, therefore, for controlling and treatment of infections that are caused by resistant microbes; discovery of new therapeutic agents is a necessary requirement. The aim of this study was assessment of antimicrobial effects of extract and essential oils of Myrtus communis against methicillin-resistant Staphylococcus aureus. 7 samples of methicillin-resistant Staphylococcus aureus were isolated from nose and throat. After assessment of antibacterial effect of extract and essential oils of Myrtus communis, the MIC and MBC also were studied by microtiterplate. The results showed that the most inhibitor concentration was 2.5 mg/ml whereas; the most lethal concentration was 5 and 10 mg/ml.
Ziba Jam zadeh; Akhtar Tavasoli; Elaheh Salar
Abstract
The genus Tulipa (Liliaceae) is distributed through Europe, Asia and North Africa. 100 species are consider ed in this genus, 1 9 species from Iran. As t here are some ambiguity in determination and delimitation of the Tulipa species , the , pollen morphology of the species were studied to be ...
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The genus Tulipa (Liliaceae) is distributed through Europe, Asia and North Africa. 100 species are consider ed in this genus, 1 9 species from Iran. As t here are some ambiguity in determination and delimitation of the Tulipa species , the , pollen morphology of the species were studied to be used in their definition. 15 native taxa of the Tulipa belonging to subgenera Eriostemones and Tulipa were studied using Light and Scanning electron microscopy . Pollen grains collected from herbarium specimens in Research Institute of Forests and Rangelands (TARI) and herbarium of pests and disease institute (IRAN) , were acetolysed and prepared for light and scanning electron microscopy. The pollen s w ere mono colpate or three aperturate. Exine in species belonging to subgenus Eriostemones were rugulate-stri a te . In subgenus Tulipa five types of ornamentation were recognized. Differences observed in pollen characters are in agreement with the division of the Tulipa into two subgenus Eriostemones and Tulipa. Palynological characters can be used, helping in dividing of Tulipa into subgenera and sections.
Mohammad Sodagar; Saiedeh Kivanlou
Abstract
Cryopreservation of fish embryos requires an optimal concentration of cryoprotectants inside all embryo compartments.In order to study the effects of cryoprotectants toxicity, two Persian sturgeon (Acipenser persicus) embryons (24 and 48 h after fertilization) were investigated using Methanol (MeOH), ...
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Cryopreservation of fish embryos requires an optimal concentration of cryoprotectants inside all embryo compartments.In order to study the effects of cryoprotectants toxicity, two Persian sturgeon (Acipenser persicus) embryons (24 and 48 h after fertilization) were investigated using Methanol (MeOH), Ethylen glycol (EG) and 1,2- propandiol (PROH) (1 to 6 M), sucrose and honey (10,15 and 20%) and polyvinyl pyrolidon (PVP) (5, 10 and 15 %). Embryos were exposed using these cryoprotectants for 5 and 10 min. Embryos were then washed and incubated until hatching. EG was found to be the most toxic cryoprotectant. Persian sturgeon embryos tolerated MeOH better than EG compared to MeOH and EG, 1,2- propandiol gave the highest hatching rate and Persian sturgeon showed lower sensitivity in two embryonic development stage (24 and 48 h after fertilization). The hatching rate decreased with increasing concentration and exposure time in all treatments except sucrose and honey. Reduced hatching rate may be a result of osmotic shock, ionic imbalance or consequence of cryoprotectants toxicity. Finally with the progress of embryonic development among various cryoprotectants, sensitivity decreased in 1,2- propandiol and polyvinyl pyrolidon compared to MeOH, EG, sucrose and honey.
Rouha Kasra kermanshahi; Faranak Mamoundad nezhad
Abstract
Acrylic fibers contain nano-silver and Nano-TiO2, are made of co-monomer of acrylonitrile .These fibers can be used in many different bases such as loom. Soil is one of the most important habitats of microorganisms which have vital role in different nutrient cycling. Some of these important microorganisms ...
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Acrylic fibers contain nano-silver and Nano-TiO2, are made of co-monomer of acrylonitrile .These fibers can be used in many different bases such as loom. Soil is one of the most important habitats of microorganisms which have vital role in different nutrient cycling. Some of these important microorganisms are Rhizobia which play an important role in cycle of nitrogen fixation and some bacilli which have cellulose enzymes and are essential in carbon cycle. In this study, R. cicil and Bacillus sp. were exposed to the acrylic fibers that contain nano-silver and Nano-TiO2, to investigate the effect of these fibers on these bacteria. Shake flask was the method used in this process and in different periods of time (0, 6, 24 hours) the colonies of bacteria were counted by drop plate method. The results showed that after 24 hours acrylic fibers which consist of nano-silver and nano-TiO2, have antibacterial effect on R. cicil and Bacillus sp. and decreased the growth of these bacteria to 72.61% and 78.09% respectively.
