Research Paper
Latifeh Poorakbar; sonia Maghsoumi Holasoo
Abstract
Salinity is one of the most important environmental factors which reduce the plants growth and their fertility around the world. In this study maize (Zea mays L.) plants grown in hydroponic culture were treated with NaCl(0, 100 and 150mM).After 1 month of treatment, growth parameters and some biochemical ...
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Salinity is one of the most important environmental factors which reduce the plants growth and their fertility around the world. In this study maize (Zea mays L.) plants grown in hydroponic culture were treated with NaCl(0, 100 and 150mM).After 1 month of treatment, growth parameters and some biochemical changes were studied in roots and shoots of plants. The results showed that with increasing NaCl concentration, leaf area and root and shoot length were decreased, while total soluble sugar, level of H2O2, malondealdyde as indicator lipid of peroxidation were increased. The activities of catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase increased in plants supplied with both concentration of NaCl. Therefore, it can be concluded that supply of NaCl induced oxidative stress by increasing production of ROS despite increased antioxidant protection in maize plants.
Research Paper
zahra Hajihassan; Azra Rabbani-Chadegani
Abstract
Actinomycin D and mitoxantrone are anticancer drugs widely used in the treatment of a variety of cancers. DNA has been introduced as a main target for these drugs which results in inhibition of both DNA replication and RNA transcription. In the cell nucleus, DNA is not naked but is complexed with histone ...
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Actinomycin D and mitoxantrone are anticancer drugs widely used in the treatment of a variety of cancers. DNA has been introduced as a main target for these drugs which results in inhibition of both DNA replication and RNA transcription. In the cell nucleus, DNA is not naked but is complexed with histone proteins producing a compact structure called chromatin. In the present study we have investigated and compared the binding affinity of these drugs to rat liver chromatin. The results of gel electrophoresis and UV-Vis spectroscopy showed that both drugs bound to chromatin but mitoxantrone exhibited higher affinity compared to actinomycin D. Disappearance of histones on the gel and reduction of the absorbencies at 608 and 440 nm, corresponding mitoxantrone and actinomycin D respectively, suggest compaction/aggregation of the chromatin. The binding isotherms demonstrated a positive cooperative binding pattern for both drugs with binding constants (ka) of 7.1×106 M-1 and 0.35×105 M-1 for mitoxantrone and actinomycin D respectively suggesting higher binding affinity of mitoxantrone to chromatin compared to actinomycin D. In addition Gibbs free energy for the binding of both drugs was negative demonstrating that the reaction is spontaneously. It is concluded that actinomycin D preferentially interacts with DNA molecule, whereas, mitoxantrone binds to nucleoprotein structure of chromatin implying the role of histone proteins in this binding process.
Research Paper
masoumeh Helmi-Seresht; sara Saadatmand; ramezanali Khavari-Nejad
Abstract
Cyanobacterium Arthrospira (Spirulina) platensis is a filamentous blue-green algae which has many nutritional and medicinal values for humans and animals. The present study investigated the effect of light intensity and pH on the productions of protein, fat, chlorophyll a and carotenoids in Spirulina ...
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Cyanobacterium Arthrospira (Spirulina) platensis is a filamentous blue-green algae which has many nutritional and medicinal values for humans and animals. The present study investigated the effect of light intensity and pH on the productions of protein, fat, chlorophyll a and carotenoids in Spirulina platensis. Pure sample of Spirulina was grown in BG-11 liquid culture medium under the influence of three optical intensities including 1500, 1000 (control), and 500 Lux and five pH treatments including 5.1, 6.1, 7.1 (control), 8.1, 9.1 and fintensity had a statistically significant impact on the amount of fat production in the cyanobacterium. However, no significant difference was observed in the amounts of the protein and photosynthetic pigments productions. The results of investigating the influence of pH changes in Spirulina platensis indicated that alkaline pH was more suitable in the productions of fat and protein than neutral pH and resulted in a significant increase in the amount of these compounds in cyanobacterium Spirulina.
Research Paper
Moje Khaleghi; Hamdolah Zarei; Mohamad Bokaeian; Saeedeh Saeidi
Abstract
Infectious diseases caused by Gram-positive bacteria Staphylococcus aureus strains that resistant to drugs, which have often a hospital source , is increasing in many countries. In this applied study, 40 persons of therapeutic Personnel including nurses, technicians, experts and patients ...
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Infectious diseases caused by Gram-positive bacteria Staphylococcus aureus strains that resistant to drugs, which have often a hospital source , is increasing in many countries. In this applied study, 40 persons of therapeutic Personnel including nurses, technicians, experts and patients from Zabol hospital and 40 students of Zabol University who were willing to participate in this research were selected. They were evaluated in terms of vectors. Samples, with separate sterile swabs were taken from the anterior portion of the nose and throat then were immediately transferred to the culture medium. Finally, by standardized tests, Staphylococcus aureus was detected. Then-PCR method, by using specific primers mecA, the Staphylococcus aureus was isolated. The results of this research showed that nasal carriers were 17/41 percent , throat carriers were 29/35 percent , nose/throat carriers were 76/11 and about 17/41 percent of all Staphylococcus aureus isolated, were resistant to methicillin. Due to the high rate of carriers of Staphylococcus aureus that resistant to methicillin, for Identifying these people and treat them in order to eradicate the infectious agent ,can be one of the important ways to prevent hospital infections that in terms of the health and decreasing mortality , social , economic and health impacts is very important.
Research Paper
Mehrdad Zarafshar; Moslem Akbarinia; Hosain Asgari; Saeed mohsen Hosaini; mehdi rahaee
Abstract
One of the best solutions to overcome drought stress is the use of wild germplasm. We aim to understand how wild pear (Pyrus boisseriana) conserves water and recovers water potential after drought. After 18 days without water, which coincides with leaf rolling, we surveyed physiological and biochemical ...
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One of the best solutions to overcome drought stress is the use of wild germplasm. We aim to understand how wild pear (Pyrus boisseriana) conserves water and recovers water potential after drought. After 18 days without water, which coincides with leaf rolling, we surveyed physiological and biochemical parameters of pear seedlings and were re-surveyed them again after a week of re-watering. After signs of leaf rolling, the relative water content decreased to a critica level (35%).In contrast to the control, RWC declined by 58% and recovered acceptably. By the end of the experiment, xylem water potential declined -2.22 Mpa, but increased watering. For osmosis adjustment in the plant cell, free proline and glucose content was increased about 5-6%. The finding showed that increasing carotenoid content is one of the strategies in response to oxidative stress, because active oxygen species could not decrease chlorophyll content. On the other hand, MDA and H2O2 content as lipid peroxidation and oxidative stress indicator respectively narrowly increased. Finally it can be concluded that increasing the carotenoid and proline content are the important mechanism for water limiting stress. It seems that this wild pear germplasm can tolerate lack of water for about 18 days. Moreover, leaf rolling sign is a good indicator of drought resistance potential.
Research Paper
Maryam Keshavarzi; Fariba Raeesi; Parinaz Ghadam
Abstract
Prosopis (Fabaceae) is one of Mimosoideae genera with 4 native and cultivated taxa with edible and forage importance in Iran. Seeds of Mesquite comprise a great amount of protein and sugar compounds. In this project electrophoretic characteristics of seed proteins of four species of Prosopis in Iran ...
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Prosopis (Fabaceae) is one of Mimosoideae genera with 4 native and cultivated taxa with edible and forage importance in Iran. Seeds of Mesquite comprise a great amount of protein and sugar compounds. In this project electrophoretic characteristics of seed proteins of four species of Prosopis in Iran as P. cineraria, P. farcta, P. juliflora and P. koelziana were studied by collecting five accessions from nature. The aim of this study was to define the diagnostic value of seed protein electrophoretic data in species separation and taxonomy in Iran. By use of SDS-PAGE method totally 22 bands were achieved. Data was analyzed and evaluated by multivariate statistical methods. It is the first seed protein electrophoretic study for Prosopis species in Iran and for P. farcta (both varieties) and P.koelziana in the world. Seed storage protein profiles were studied and species separation was confirmed. Seed stored Albumin was studied for the first time in Prosopis farcta var. farcta, P. farcta var. glabra and P. juliflora. There were differences in electrophoretic profiles of two varieties. P. juliflora represented a separate position from other taxa. P. koelziana show more similarities to P. juliflora in seed storage protein profiles. Diagnostic value of electrophoretic data is discussed.
Research Paper
Khadigeh Nabighahfarokhi; Mansoreh Ghaeni; Ladan Zaheri
Abstract
Goat fishes are marine fish that live in shallow water and they have distributed through the Persian Gulf and Oman Sea. They have useful unsaturated fatty acids. There are many document about classification and ecological study but we didn`t find any paper on fatty acid profile. In this research ...
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Goat fishes are marine fish that live in shallow water and they have distributed through the Persian Gulf and Oman Sea. They have useful unsaturated fatty acids. There are many document about classification and ecological study but we didn`t find any paper on fatty acid profile. In this research goat fishes catched from Mahshahr fishery port in two seasons autumn and spring by local fisherman by gillnet. Thirty fishes were chosen accidently and kept them in ice and transferring to the laboratory. The amount of their fatty acids was measured with GC_FDI instrument and two important indices (IT and IA) were calculated. The results were compared by one way Variance analysis and a test called (Tocky and Doncken). Differences between two season (spring and autumn)'s fatty acids were significant. And the amount of unsaturated fatty acids in autumn was more than spring.
