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he Role of Growth Regulators in Optimization of Callus Production in Medicinal Plant of Red Rubin Basil (Ocimum basilicum L.)

Document Type : Research Paper

Authors

1 Associate Professor, Department of Agricultural Sciences, Payam Noor University, Tehran-Iran.

2 Assistant Professor, Department of Biology, Payam Noor University, Tehran-Iran.

3 Senior Expert, Department of Iranian Medicine, Aja University of Medical Sciences, Tehran, Iran.

4 Professor of Agricultural Biotechnology Department, Payam Noor University, Tehran-Iran.

Abstract

Background and purpose: Basil is annual herb it belongs to the mint family and it has 30 species; therefore, in this research, optimization of tissue culture conditions of purple basil plant for callus production, identification of callus induction percentage, growth rate, growth speed and relative water content of callus was done through tissue culture. Materials and Methods: The callus induction optimization experiment was carried out as a factorial in the form of a completely randomized design with 4 factors, micro samples and three levels of BA, IAA and IBA hormones. Findings: The results showed that the highest percentage of callus induction (88%) was related to the culture medium containing 2 mg/L IBA and 2 mg/L BA. The effect of hormonal compounds on the four investigated characteristics of callus generation percentage, growth speed, relative water content and growth rate (dry weight) has had a significant effect, which can be used to select the best combination in callus formation and tissue culture optimization, and finally the future goals of the breed. Also, the interaction effect of two explant factors and hormonal compounds only had a very significant effect (P≤1%) on the growth rate index. Conclusion: The quality of the induced calluses indicates the correct selection of explants and suitable hormonal compounds. Therefore, there is a direct relationship between callus production and growth regulators, and also according to the type of plant studied and the amount of hormone present in it, the amount of growth regulators used in callus induction will be different.

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References
 
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Applied Biology
Volume 36, Issue 2 - Serial Number 76
September 2023
Pages 113-125
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