Document Type : Research Paper

Authors

• Fariba Hasnvand
• Masoud Haidarizadeh

Abstract

Cereal and legume kernels contain many α-amylase inhibitors which have potential to inhibit the α-amylase activity from variant sources. The subject of this study was partial purification of α-amylase inhibitors of red bean seeds. In this regard, we precipitated extractions as three parts with different saturation percentages of ammonium sulfate. Then, we dialyzed, in order to remove ammonium sulfate and better checking of their inhibitory activity upon on Aspergillus oryzae α-amylase. By assaying rate of inhibitory activity of each sample on the basis of their absorbance in 540 nm and comparison to absorbance of negative control, we saw that only (30-60)% fraction of each extract had inhibitory activity upon on defined α-amylase. Inhibitory of these fractions for red bean extract were 89.32 percentage. For more characterization of these inhibitors, we conducted HPLC and SDS-PAGE analyses. By assaying the activity of these inhibitors upon on other α-amylase such as salivary and pancreatic α-amylases, may be it is possible to use these α- amylase inhibitors such as Acarbose and Miglitol for treatment of type II diabetes. Also the results might be applied in agriculture to produce transgenic plants that express α-amylase inhibitors against α-amylase of pests.

Keywords

• α-amylase inhibitors
• partial purification
• seed protein
• Red bean kernel