Document Type : Research Paper

Authors

1 Master of Biophysics, Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Profesor Asistente de Biofísica, Departamento de Biología, Facultad de Ciencias Básicas, Universidad Islámica de Azad, Rama de Ciencia e Investigación, Teherán, Irán

3 Profesor Asistente de Bioquímica, Grupo de Investigación en Fotodinámica, Centro de Investigación Láser en Medicina, Instituto de Investigación Yara, Universidad Jihad, Universidad de Ciencias

4 Profesor Asistente de Biofísica, Grupo de Investigación Médica Láser, Centro de Investigación Láser en Medicina, Instituto de Investigación Yara, Universidad Jihad, Rama de Ciencias

Abstract

The aim of this study was to investigate the effect of Cartap hydrochloride on the structure and function of hemoglobin to determine the molecular mechanism of toxicity of toxin. In this research, the effect of Cartap on the structure and aggregation of human hemoglobin was investigated by UV-visible spectroscopy, thermal spectroscopy, florescent spectroscopy and Attenuated total reflection Fourier transform infrared (ATR‐FTIR) spectroscopy in both titration and incubation conditions and in different concentrations of toxic. Based on UV-visible spectroscopy, results revealed that absorption spectra were changed by adding different concentrations of toxin. Analysis of the levels of oxy-deoxy and met hemoglobin in presence of various concentration of Cartap showed a decrease in active hemoglobin species. Moreover, the aggregation and hemolysis of red blood cells increased in presence of Cartap. In florescent spectroscopy minor degradation of Heme happened and in the second structure of hemoglobin has not altered in presence of toxin.

Keywords

 
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