Document Type : Research Paper

Authors

1 Associate Professor, Department of Biological science, Alzahra University, Tehran, Iran

2 Assistant Professor, Department of Biological science, Alzahra University , Tehran, Iran

3 Graduated student, Department of Biological science, Alzahra University, Tehran, Iran

Abstract

The genus Consolida(DC.) S. F. GRAY of Gray  is a member of Ranuculaceae family, Delphinieae tribe.. It comprises approximately 52 species which almost all of them are weed and includes the members of the genus Aconitella Spach.  The genus distributed in Iran with 24 species, Iran is thus one of the richest countries for the genus in South-West Asia. Consolida has separated from Delphinium by De Candolle  based on single-spurred petals , one follicle and annual life cycle and has occurred in separate section. Finally it introduced as a separate genus by Gray in 1821.
   In this study the petiole anatomy of 16 species of the genus Consolida have investigated. Three population from each species have examined. After pre-treatment of  the material , hand sections prepared and Carmen and methyl green solution used for staining the materials. Olympus light microscope used for study and photography. Our results showed that the presence of : angle, collenchymas layer below the epidermis, the number of vascular bundles LB, vascular sheath type, mechanical tissue between the vascular bundles and  type of vascular bundles  and indumentums of petiole are diagnostic characters for distinguishing the species. Based on anatomical results the species of two subgenera (Consolida and Aconitella) have separated and this confirmed  the results of molecular systematic study of the genus.

Keywords

Boissier, E. (1867) Flora Orientalis voil primum, Thalamiflorae. Basilieae.
Constantinidis, T., Paras, G. K. & Kamari, G. (2001)  Seed morphology in relation toinfrageneric classification of Consolida (Dc.)S. F. GRAY (Ranunculaceae). Flora, 196: 81–100.
Davis, P. H., Mill, R.R. &  Tan Kit (eds.((1988) Flora of Turkey and the East Agent Islands vol. 10 (supplementum). Edinburgh University Press. Edingurgh.
De Candolle, A. P. (1824) Prodromus systematics naturalis regni vegetabilis, pars prima, Parisiis.
Gostin, I. N. (2011)  Anatomical and micromorphological peculiaritiesof Adonis vernalis L. (Ranunculaceae). Pakistan Journal of  Botany, 43:811 – 820.
Iranshahr, M., Rechinger, f., K.H., Riedl, H. (1992) Consolida. In: Rechinger, K. H. (ed.). Ranunculaceae, Flora Iranica, No. 171: 44-114. Akademische Druck. U. Verlagsanstalt. Graz, 89-114.
Jabbour,  F.,  Renner,  S.S.  (2012)  A phylogeny of Delphinieae (Ranunculaceae) shows that Aconitum is nested within Delphinium and that Late Miocene transitions to long life cycles in the Himalayas and Southwest China coincide with bursts in diversification. Molecular Phylogeny and Evolutio, 62: 928–942.
Jabbour, F., Renner, S.S. (2011a) Consolida and Aconitella are an annual clade of Delphinium (Ranunculaceae) that diversified in the Mediterranean basin and the Irano-Turanian region. Taxon, 60, 1029–1040.
Kavathekar, K.Y. and Pillai, A. (1976) Studies on the developmentalanatomy of Ranales. II – Nodal anatomy of certainmembers of Annonaceae, Magnoliaceae, Menispermaceae andRanunculaceae. Flora. 165: 481 – 488.
Kokdil, G. Ilcim, A., Ozbilgin, B., Uygun, C.(2006)  Morphology and stem anatomy of somespecies of genus Nigella L. in Turkey. Ankara Ecz Üniversitesi Eczacılık Fakültesi Dergisi, 35: 19 – 41.
Munz, P.A.( 1967a). A synopsis of African species ofDelphinium and Consolida. Journal of  Arnold Arbratum, 48:30–55.
Munz, P.A. (1967b) A synopsis of Asian species of Consolida (Ranunculaceae). Journal of  Arnold Arbratum , 48: 159–202.
Nevskii,  S. A. (1937) Delphinium L. In: Shishkin, B.K. (ed.), Flora of the Ussr, 7: 79–143. – Moskva,Leningrad.
Novikoff , A.V. (2009b)  Anatomical organization of petioles of some species from the genus Aconitum L. Plant introduction,selection and protection (Proc. II Int. Sci. Conf., Ukraine, Donetsk, 6 – 8 Oct 2009): 197 – 198.
Novikoff , A.V. (2010)  Some features of anatomical structure of thestem of Aconitum moldavicum Hacq. and Aconitum lycoctonumL. em. Koelle (Ranunculaceae). Studia Biologica, 4: 109 – 114.
Novikoff , A.V. and Mitka, J. (2011) Taxonomy and ecology of thegenus Aconitum L. in the Ukrainian Carpathians. Wulfenia, 18: 37 – 61.
Novikoff , A.V. (2011a) Nodal anatomy of the genus Aconitum L.(Ranunculaceae). Proc. V Int. Conf. Young Sci., (Ukraine, Odessa, 13 – 17Jun 2011: 47 – 48).
Novikoff , A.V. (2011b) Nodal anatomy of the representatives fromthe section Aconitum subgenus Aconitum ( Aconitum L., Ranunculaceae). Proceedings of the United States National Museum, 27: 111 – 125.
    Novikoff , A.V. (2015) Th e leaf blade anatomy of some Aconitum (Ranunculaceae) in Eastern Carpathians. Nordic Journal of Botany, 33: 633–640.
Pakravan, M., Dastpak, A., Sonboli, A., & Khalaj, Z. (2018) A taxonomic reassessment of Consolida (Ranunculaceae) species: insight from morphological and molecular data.Journal of Genetic Resources, 4(1): 14-25.
Sheidai, M.,  Azizian, D., Ghorbani Nohooji, Mahboobeh K. (2011)Taxonomic value of stem anatomical characters in classification of some Adonis (Ranunculaceae) species in Iran. Taxonomy and  Biosysematics, 3(6): 69-78.
Soo, R. Von (1922) Über die mitteleuropäischenArten und Formen der Gattung Consolida (DC.)S. F. Gray. Oesterreichische botanische Zeitschrift, 71: 233–246.
Smith, E. P. (1928) A comparative study of the stem structure of the genus Clematis , with special reference to anatomicalchanges induced by vegetative propagation. Transactions of the Royal Society of Edinburgh, 55: 643 – 664.
Tavassoli, A.,  Pakravan, M., Kiarostami, K . and Poorhabibian, R. (2012) Karyotype analysis in some species of Consolida (Ranunculaceae) from Iran. Feddes Repertorium, 123 (4), 257-263
Tobe, H. (1980) Morphological studies on the genus Clematis Linn. VIII. Floral and infl orescence anatomy in Clematis patens with eight-sepaled flowers. The Botanical magazine, Tokyo
Trifonova, V.I. (1990) Comparetive biomorphological study of the taxonomy and phylogeny of the genera Consolida (DC.) S.F.Gray and Aconitella Spach. Collectanea Botanica (Barcelona), 19: 97-110.
Tumanian, S. A. (1965) The peculiarities of anatomical structure ofthe stem and leaf in species of Aconitum L. Trudy Glavnago Botanicheskago Sada. (Bull. Main Bot. Gard.) 59: 59 – 66.