Isolation and Identification of Phytase Producing  Staphylococcus pasteuri from Rhizosphere of Wheat

Fooladi, Jamshid; Fayazi Hossini, Nashmin; Kiarostami, Khadijeh

doi:10.22051/jab.2018.3708

Document Type : Research Paper

Authors

¹ Assistant Professor of Biology, Alzahra University, Tehran, Iran

² Department of biology, Faculty of basic sciences, Alzahra university, Tehran,Iran

³ Phd dissertational Phylosophy, Alzahra University, Tehran, Iran

https://doi.org/10.22051/jab.2018.3708

Abstract

Phytases are enzymes capable of hydrolyzing phytic acid to less-phosphorylated myo-inositol derivates. Phytic acid The major storage form of phosphorous in plants. Monogastric animals, such as pig, poultry and fish are not able to metabolize phytic acid. Adding phytase to diet monogastric animals lead to increased avalibility of phosphorus. The objective of the present study is to isolate and characterize phytase producing bacterial strains from rhizosphere of wheat. Phytase production was determined by production of clear zones around the colonies on the sodium phytate containing medium and the best phytase producing strain was identified using molecular analysis. Also, the isolate was tested for P-solubilizing potential using National Botanical Research Institute Phosphate (NBRIP) containing tricalcium phosphate as the sole P source. Among the 11 bacterial isolates, forming clear peripheral zones on a turbid agar plate, Staphylococcus pasteuri isolated able high potential for phytase production.This study showed that , Staphylococcus pasteuri capable production phytase and phosphatase enzyme.

Keywords

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Applied Biology

Isolation and Identification of Phytase Producing Staphylococcus pasteuri from Rhizosphere of Wheat

References

References

Volume 31, Issue 1 - Serial Number 55
July 2018
Pages 257-270

Isolation and Identification of Phytase Producing Staphylococcus pasteuri from Rhizosphere of Wheat

References

References

Volume 31, Issue 1 - Serial Number 55July 2018Pages 257-270

Volume 31, Issue 1 - Serial Number 55
July 2018
Pages 257-270