Document Type : Research Paper
Authors
1 Assistant Professor ,Crop Physiology, University of Maragheh, Maragheh, Iran
2 Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
3 Assistant Professor, Department of Agronomy and Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
Abstract
In order to evaluate the effect of ascorbic acid and folic acid on germination parameters
under drought and salinity stress, two separate experiments was carried out in completely
randomized factorial design with Azar2 Wheat cultivar. In this experiment, wheat seeds
were pretreated for 16 hours with concentration of zero (control), 0.5 and 1 mM of ascorbic acid and 25 and 50 μM folic acid. Seeds were then dried at room temperature until the
initial weight. To apply drought and salinity stress the solution of polyethylene glycol 6000
at 0 (control), -6 and -12 bar and sodium chloride at zero, 75 and 150 mM concentration
were used, , respectively. Subsequently, petri dishes containing wheat seeds were kept at
temperature of 24±1°C, 70% relative humidity and darkness condition for 8 days. After the
mentioned time, germination percentage, germination rate, root and shoot length and
seedling dry weight were measured. The results showed that seed treatment with vitamins, even in favorable conditions, with reduction of the loss of seed reserves, can help
more allocation of seed reserves at formation of seedling structure, and cause to generate
of stronger seedlings. Under drought and salinity stress conditions, seed treatment with
ascorbic and folic acid, by creating appropriate conditions, can prevent damages caused by
generate reactive oxygen species or prevent from their production, which the result of its,
is the absent expression of oxidative stress on in seed germination. These factors result in
increasing growth respiration rate and formation of stronger seedlings. Therefore, increasing the amount of mentioned vitamins in seeds can improve the metabolic function, especially in difficult environmental situations and contribute in better seedling establishment.
Keywords
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