Authors
1 Malek Ashtar University
2 University of Tehran
3 Al-Zahra University
Abstract
The aim of the present study was extraction and purification of cyclotides from Iranian Viola odorata and examination of their antimicrobial effects against Gram-positive S. aureus PTCC 1431. In the current study, the cyclotide was extracted and purified by fractionation method using SPE-C18 column. Determination of cyclotide purification degree and molecular weight was done by RP-HPLC and tricine SDS-PAGE methods, respectively. Antimicrobial effects of these peptides were studied by radial diffusion assays (RDAs). The results showed that antimicrobial effects of cyclotides were similar to vancomycin and amikacin. It seems that future studies might reveal and propose the use of these cyclotides in treatment of staphylococcal infections
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