Optimization of Bio-Vanillin with Native Isolated Psychrobacter sp.  Strain CSW4 Using the Taguchi Method under  Resting Cells

Ashengarf, Marahem; Nahvi, Eraj; Amini, Jahanshir

doi:10.22051/jab.2014.1126

Document Type : Research Paper

Authors

https://doi.org/10.22051/jab.2014.1126

Abstract

In the recent years, the use of microbial biotransformation processes to achieve specific properties of vanillin and access to the natural vanillin origin has been a lot of attention. In the present study, Taguchi method was employed for optimizing the biotransformation of isoeugenol to vanillin under resting cells of native isolate Psychrobacter sp. strain CSW4. Five factors, i.e. initial isoeugenol concentration, initial dry biomass, co-substrates (glycerol, yeast extract and tryptone), initial NaCl concentration and metal ions (Cu, Zn and Co, were selected and experiments based on an orthogonal array layout of L18 were performed. Vanillin produced in the biotransformation reaction mixture was analyzed by HPLC method. Optimization of the process by Taguchi method showed that highest impact factors with importance priority of NaCl concentration, initial isoeugenol concentration, glycerol as substrate, cobalt ion and initial dry biomass were determined as optimum conditions for the biotransformation isoeugenol into vanillin, respectively. Under these optimized conditions, the highest vanillin concentration (1.016 g/L) obtained after 24-h reaction biotransformation. The molar yield of vanillin produced from isoeugenol was 43.8 %. Results of this study indicate that if optimization of medium composition is performed to balance the cell growth and vanillin amount, reasonable yields of vanillin have been achieved, without addition of any toxic organic solvent or other chemicals which increase the cost of production.

Keywords

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Applied Biology

Optimization of Bio-Vanillin with Native Isolated Psychrobacter sp. Strain CSW4 Using the Taguchi Method under Resting Cells

References

References

Volume 25, Issue 1
July 2012
Pages 1-16

Optimization of Bio-Vanillin with Native Isolated Psychrobacter sp. Strain CSW4 Using the Taguchi Method under Resting Cells

References

References

Volume 25, Issue 1July 2012Pages 1-16

Volume 25, Issue 1
July 2012
Pages 1-16