Molecular characterization of algD, lasR, and rhlR genes in Pseudomonas aeruginosa  isolated from hospitalized patients

Adheem Khoman, Hossein; Behboodian, Bita; Dolatabadi, Samane

doi:10.22051/jab.2024.48523.1652

Document Type : Research Paper

Authors

¹ MSC. Department of Biology, Science and Research Branch, Islamic Azad University Tehran, Iran

² Professor. Department of Animal Science, Kashmar Branch, Islamic Azad University, Kashmar, Iran

³ Assistant Professor.Department of Microbiology, Neyshabur branch Islamic Azad Univercity, Neyshabur, Iran

10.22051/jab.2024.48523.1652

Abstract

Background: Pseudomonas aeruginosa is an opportunistic pathogen that causes various infections, especially in patients hospitalized in intensive care units. Antibiotic resistance and biofilm production are known as two important factors in the pathogenesis of this bacterium.
Methods: 150 clinical samples were collected from patients of Quchan Hospital, the isolates were identified and isolated based on standard microbiological and biochemical methods. Investigation of multidrug resistance (MDR) was done by disk diffusion method. PCR (polymerase chain reaction) technique was used to identify algD, lasR and rhlR genes.
Findings: According to the obtained results, the percentage of resistance to cefixime antibiotic was 86% and gentamicin was 72%. The lowest percentage of resistance to cefepime was reported with 34%. 20 isolates were insensitive to imipenem and meropenem. 60% of isolates had MDR phenotype. 100% of the isolates carried the algD gene, 90% of the isolates carried the rhlR gene, and finally 95% of the isolates carried the lasR gene.
Conclusion: The results showed high presence of algD, rhlR and lasR genes in Pseudomonas aeruginosa isolates. The high prevalence of genes involved in signaling and biofilm production also affects virulence and antibiotic effectiveness. Pseudomonas aeruginosa biofilm-related infections are considered a major problem in antibiotic therapy. In this regard, other alternatives such as anti-biofilm or antiviral candidates are needed.

Keywords

Main Subjects

Microbiology

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Applied Biology

Molecular characterization of algD, lasR, and rhlR genes in Pseudomonas aeruginosa isolated from hospitalized patients

References

References

Volume 38, Issue 2 - Serial Number 38
April 2025

Molecular characterization of algD, lasR, and rhlR genes in Pseudomonas aeruginosa isolated from hospitalized patients

References

References

Volume 38, Issue 2 - Serial Number 38April 2025

Volume 38, Issue 2 - Serial Number 38
April 2025