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Isolation and Molecular Identification of Plant Growth-Promoting Bacteria from Phyllosphere and Rhizosphere of Triticum aestivum L.

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Biology, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran

2 MSC.Department of Microbiology, Faculty of Basic Sciences, Kazerun Branch, Islamic Azad University, Kazerun, Iran

Abstract

Introduction: A significant number of soil bacteria are capable of nitrogen fixation, phosphate solubilization and auxin production. Such bacteria can play an effective role in improving plant growth. The purpose of this research was to isolate, identify and investigate the performance of such bacteria on the growth rate of lentil. Materials and methods: Thirty bacterial samples were isolated from phyllosphere and rhizosphere of wheat. Nitrogen-free culture medium was used to identify nitrogen-fixing bacteria, and NBRIP culture medium was used to evaluate the ability of solubilization of phosphate. The ability of the isolates to produce auxin was investigated by the Salkowski method. Also, the effect of two superior isolates on the longitudinal growth of stem and root of lentil were investigated. Results: Out of 14 phyllospheric and 16 rhizospheric isolates, 2 isolates A and B, which were isolated from the rhizosphere and phyllosphere of wheat, respectively, and had the highest ability for the desired parameters, were identified based on sequencing a portion of 16S rDNA gene. Isolate A with nitrogen fixation and phosphate solubilization ability was 98% similar to Bacillus cereus and isolate B with auxin production ability was 99% similar to Arthrobacter globiformis. isolate A by 8% and isolate B by 50% were able to increase the longitudinal growth of lentil stem. Discussion and Conclusion: The wheat rhizosphere have bacteria capable of nitrogen fixation, phosphate solubilization, and auxin production. Applying such strains can play an effective role in improving plant growth and reducing the use of chemical fertilizers.

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References
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Applied Biology
Volume 37, Issue 3 - Serial Number 81
October 2024
Pages 36-46
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