Nanoemulsification of Thyme essence by a mixture of emulsifiers and evaluation of the antibacterial activity of E.coli DH5α

Shakeri, Fatemeh; Moradian, Fatemeh; Mehraban Jobini, Pouyan; Rostami, Mehran

doi:10.22051/jab.2023.45307.1594

Document Type : Research Paper

Authors

¹ Master's degree, Department of Basic Sciences, Sari University of Agricultural Sciences and Natural Resources, Mazandaran, Sari

² Associated professor, Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran.

³ Assistant professor, Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran.

⁴ Associated professor, Department of Nano Materials and Nano Coatings, Institute for Color Science and Technology, Tehran, Iran.

https://doi.org/10.22051/jab.2023.45307.1594

Abstract

Subject: The present study was conducted with the aim of nanoemulsifying Thymus vulgaris essential oil to increase its stability and investigate its antibacterial effects on E. coli DH5α bacteria. Methods: First, the initial microemulsion was prepared in the oil phase and then it was transformed into a nanoemulsion by ultrasound. The amount of surface charge, viscosity, particle size and morphology of essential oil nanoemulsion was investigated by zeta potential device, DLS and TEM electron microscope. Results: The results showed that the zeta potential of nanoemulsion containing 10 and 30% essential oil was -11.5 and -11.1 mV, respectively. The morphology of the nanoparticles of both samples was spherical and the average size of the nanoemulsion nanoparticles with 10% and 30% essential oil was 43 and 30 nm, respectively. The results of the antibacterial effects of nanoemulsions showed that the nanoemulsion containing 10% essential oil (v/v), with a volume of 30 µl, and the nanoemulsion containing 30% essential oil (v/v), with a volume of 20 µl, had approximately 50% inhibitory effect (IC50) on the growth of E. coli DH5α. Also, the primary essential oil showed approximately 50% inhibition (IC50) on bacterial growth in 20 µl. Conclusion: According to the stability of the produced thyme essential oil nanoemulsions, it can be concluded that the antibacterial effects of this compound have increased compared to the original essence.

Keywords

Main Subjects

Herbal

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Applied Biology

Nanoemulsification of Thyme essence by a mixture of emulsifiers and evaluation of the antibacterial activity of E.coli DH5α

References

References

Junyaprasert V. B. and Morakul B. (2015). Nanocrystals for enhancement of oral bioavailability of poorly water-soluble drugs. Asian Journal of Pharmaceutical Sciences. 10(1): 13-23.

Volume 37, Issue 2 - Serial Number 80
July 2024
Pages 61-72

Nanoemulsification of Thyme essence by a mixture of emulsifiers and evaluation of the antibacterial activity of E.coli DH5α

References

References

Junyaprasert V. B. and Morakul B. (2015). Nanocrystals for enhancement of oral bioavailability of poorly water-soluble drugs. Asian Journal of Pharmaceutical Sciences. 10(1): 13-23.

Volume 37, Issue 2 - Serial Number 80July 2024Pages 61-72

Volume 37, Issue 2 - Serial Number 80
July 2024
Pages 61-72