Effect of Alpha-ketoglutarate on the proliferation behavior of breast cancer cells in vitro

Rashid Kalkhoran, Zahra; TALKHABI, MAHMOOD; Azizi, Vahid

doi:10.22051/jab.2024.45435.1596

Document Type : Research Paper

Authors

¹ MSC. Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

² Assistant Professor, Department of Animal Sciences and Marine Biology, Faculty of Biological Sciences and Technology, Shahid Beheshti University, Tehran, Iran

10.22051/jab.2024.45435.1596

Abstract

Introduction: Breast cancer is the most common type of cancer among women in the world. Research is ongoing to find new and less risky ways to treat breast cancer. Recent studies on the effect of Alpha-ketoglutarate (AKG) on various cancers have shown its inhibitory effect on some cancers. Here, the effect of AKG on the proliferation and migration of 4T1 breast cancer cells was investigated. Methods: 4T1 cells were treated with different concentrations of AKG, the cell viability was calculated using the MTT test, and 160 μM of AKG was selected. 4T1 cells were cultured in the presence and absence 160 μM AKG, and the growth of 4T1 cells, population doubling time, colony forming potential, cell cycle profile and cell migration rate were investigated. Result and discussion: The results showed that AKG reduces the growth of 4T1 cells and increases the population doubling time in the treatment group compared to the control group. Also, AKG decreased the colony forming potential of 4T1 cells (in terms of colony number and size). AKG caused changes in the cell cycle profile in the treatment group, and the results of the scratch repair test also showed the effects of AKG in reducing the ability of cell migration in the treatment group compared to the control. The results of this study showed that AKG can reduce the survival and proliferation of breast cancer cells. It seems that the use of AKG along with other available treatments can help to control and treat breast cancer.

Keywords

Main Subjects

animal

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Applied Biology

Effect of Alpha-ketoglutarate on the proliferation behavior of breast cancer cells in vitro

References

References

Volume 37, Issue 2 - Serial Number 80
July 2024

Effect of Alpha-ketoglutarate on the proliferation behavior of breast cancer cells in vitro

References

References

Volume 37, Issue 2 - Serial Number 80July 2024

Volume 37, Issue 2 - Serial Number 80
July 2024