Molecular tracing of caviar obtained from sturgeon species to identify the original caviar and species of sturgeon caviar

Jamshidi, Shirin; Hassanzadeh Saber, Mohammad; Monsef Shokri, Maryam

doi:10.22051/jab.2023.42758.1545

Document Type : Research Paper

Authors

¹ Assistant Professor, Department of Genetics and Biotechnology, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

² Instructor, Department of Genetics and Biotechnology, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

³ Assistant Professor, Department of Physiology and Biochemistry, International Sturgeon Research Institute, Iran Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran

10.22051/jab.2023.42758.1545

Abstract

Introduction: Identifying the species of sturgeons, the non-mixing of multi-species caviar in products made from sturgeons' caviar is one of the most important challenges in the legal and international trade of this product. One of the ways to identify the original caviar and correctly label caviar products is the use of barcodes and especially molecular barcode tracking.
Methods: In this research, fin tissue samples of Russian and Persian sturgeon, Ship, Sterlet, stellate and Siberian sturgeon, as well as their caviar samples, were used to investigate species identification markers of Caspian Sea sturgeons. DNA extraction was performed from three caviar eggs of each species and pure sturgeons fin tissue, and two types of primers were selected based on mitochondrial genes.
Results and discussion: sturgeon's marker amplified a 138 new nucleotide fragment using PCR method in all species based on COI gene, but the species-specific marker amplified different sized fragments based on D-loop gene. These fragments were reproduced in both caviar and fin tissue of sturgeons. The overall results showed that the amplified fragment of the sturgeon's marker and the species-specific marker in pure sturgeons can indicate the pure fish species.

Keywords

Main Subjects

Biotechnology

References

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Applied Biology

Molecular tracing of caviar obtained from sturgeon species to identify the original caviar and species of sturgeon caviar

References

References

Volume 36, Issue 3 - Serial Number 77
February 2024
Pages 40-48

Molecular tracing of caviar obtained from sturgeon species to identify the original caviar and species of sturgeon caviar

References

References

Volume 36, Issue 3 - Serial Number 77February 2024Pages 40-48

Volume 36, Issue 3 - Serial Number 77
February 2024
Pages 40-48