Design, synthesis and evaluation of antibacterial activity of peptide polymer based on branched PEI as a biomimetic polymer

Mirzaei, Samira; Alvandi, Hale; Rezayan, Ali Hossein; Bideh, Yasamin; Riazi, Gholam Hossein; Zarabi, Mehdi

doi:10.22051/jab.2022.39975.1484

Document Type : Research Paper

Authors

¹ MSC.Division of Nanobiotechnology, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

² PHD student.Division of Nanobiotechnology, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

³ Professor.Division of Nanobiotechnology, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

⁴ Assistant professor.Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

⁵ Professor.The Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

⁶ Assistant Professor.Division of Nanobiotechnology, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

https://doi.org/10.22051/jab.2022.39975.1484

Abstract

Introduction: Biomimetic means imitating models, systems, and elements of nature to solve complex human problems. This field of science has led to the creation of new technologies inspired by biological solutions in macro and nano sizes. Thousands of people die every year due to diseases caused by antibiotic resistance. Therefore, the use of antimicrobial peptides has become particularly important. Methods: in this study, branched polyethyleneimine polymer has been used as a biomimetic polymer of antibacterial peptides. In order to increase the antibacterial properties, the amino acids valine and lysine were used to modify the surface of branched polyethyleneimine. 1 H-NMR and FTIR were used to prove the binding of amino acids to the polymer. Antibacterial activity of the modified polymer by two methods of MIC test and growth inhibition zone on gram-positive bacteria S. aureus and B. subtilis and gram-negative bacteria E. coli and P. aeruginosa were examined. Results and discussion: Under optimal conditions, the concentration of branched polyethyleneimine polymer was 8 μl, and the amino acid valine to lysine ratio was 1: 6. Polyethyleneimine polymer modified with the amino acids valine and lysine inhibited bacterial growth concentration-dependent (p <0.05). 5 mg/ml of this polymer inhibits the growth of bacteria by more than 70%. The diameter of the inhibition zone of this polymer in examined bacteria reached about 15-20 mm. The antibacterial activity of this polymer was comparable to that of the antibiotic cefepime. It seems that this polymer can be used as an antibacterial polymer.

Keywords

Main Subjects

Biotechnology

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Applied Biology

Design, synthesis and evaluation of antibacterial activity of peptide polymer based on branched PEI as a biomimetic polymer

References

References

Volume 35, Issue 4 - Serial Number 74
February 2023
Pages 133-149

Design, synthesis and evaluation of antibacterial activity of peptide polymer based on branched PEI as a biomimetic polymer

References

References

Volume 35, Issue 4 - Serial Number 74February 2023Pages 133-149

Volume 35, Issue 4 - Serial Number 74
February 2023
Pages 133-149