Isolation and characterization of phosphate solubilizing bacteria from phosphate mines and investigation on their soil phosphate solubilization potential

Karami, Yaghoobali; Samadi, Abbas; Falah Nosrat Abad, Alireza; Sepehr, Ebrahim; Barin, Mohsen

doi:10.22051/jab.2021.34708.1400

Document Type : Research Paper

Authors

¹ PhD Student Dept. of Soil Science, Faculty of Agriculture, Urmia University, Urmia, Iran

² Professor, Dept. of Soil Science, Faculty of Agriculture, Urmia University, Urmia, Iran;

³ Associate Professor Soil and Water Research Institute, AREEO, Karaj, Iran

⁴ Associate Professor, Dept. of Soil Science, Faculty of Agriculture, Urmia University, Urmia, Iran

⁵ Assistant Professor, Dept. of Soil Science, Faculty of Agriculture, Urmia University, Urmia, Iran

https://doi.org/10.22051/jab.2021.34708.1400

Abstract

The aim of this study was to isolate and characterize phosphate-solubilizing bacteria from two phosphate mines and to investigate their soil phosphate solubility ability. Eighty five colonies with different morphologies were isolated and purified using the liquid Sperber culture medium enriched with Yazd’ Esphordi soil phosphate (YESP) and a form of diluents series in the Sparber solid culture medium enriched with YESP. Four out of 85 isolates (Rpy: from Esfordi mine and Tkd/4, Ggd/4 & Rpd/4 from Jiroud mine) with the highest amount of dissolved phosphorus in the Sperber liquid medium enriched with YESP as superior isolates were selected. With using 16S rRNA gene sequencing the isolates Tkd/4, Rpd/4, Rpy and Ggd/4 were recognized very closely (more than 99%) to Curtobacterium flaccumfaciens, Sphingobium yanoikuyae, Bacillus pumilus and Pantoaea aglomerans genera respectively. The multifold release of phosphorus by these isolates compared to the control from three phosphate sources showed that these bacteria can dissolve other phosphate sources.

Keywords

20.1001.1.22517901.1400.34.3.5.1

Main Subjects

Microbiology

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Applied Biology

Isolation and characterization of phosphate solubilizing bacteria from phosphate mines and investigation on their soil phosphate solubilization potential

References

References

Volume 34, Issue 3 - Serial Number 69
November 2021
Pages 82-97

Isolation and characterization of phosphate solubilizing bacteria from phosphate mines and investigation on their soil phosphate solubilization potential

References

References

Volume 34, Issue 3 - Serial Number 69November 2021Pages 82-97

Volume 34, Issue 3 - Serial Number 69
November 2021
Pages 82-97