Document Type : Research Paper

Authors

1 Shahid Bakeri High Education Center if Miandoab, Urmia University, Urmia, Iran

2 Graduated of Medicinal Plants Engineering, Shahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, Iran

Abstract

Spearmint (Mentha spicata L.) and Horsemint (Mentha longifolia L.) have abundant use in food expenses and Iranian Traditional Medicine. Tha aerial parts of both plants were collected and essential oil was isolated by hydrodistillation method (Clevenger). The extraction was conducted by maceration method (aqueous, hydroalcoholic and ethanolic solvents) and soxhlet method (ethanolic solvent). Total phenolic compounds content, Total flavonoid content, antioxidant capacity and free radical inhibition were measured. The results showed that total phenolic compounds and flavonoid content, total antioxidant capacity and percentage of free radical inhibition in applied extraction methods was different (p < 0.05). The highest total phenolic compounds and flavonoid was obtained by soxhlet method in spearmint. The highest total antioxidant capacity were belong to hydroalcoholic extract in spearmint. Soxhlet extraction method is recommended because of its low processing cost, simplicity of operation, consume lesser time and solvent compared to other traditional methods such as soaking or percolation to extract phenolic compounds using other solvents. The use of a two-solvent system in the soaking process with other solvents requires further research to achieve high antioxidant properties.

Keywords

Abad-García, B., Berrueta, L. A., López-Márquez, D. M., Crespo-Ferrer, I., Gallo, B. and Vicente, F. (2007) Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices. Journal of ChromatographyA. 1154(1): 87-96.
Akroum, S., Bendjeddou, D., Satta, D. and Lalaoui, K. (2009) Antibacterial activity and acute toxicity effect of flavonoids extracted from Mentha longifolia. American Journal of Scientific Research. 4(2): 93-96.
Alara, O. R., Abdurahman, N. H., and Ukaegbu, C. I. (2018) Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity. Journal of Applied Research on Medicinal and Aromatic Plants, 11, 12-17.
Al-Okbi, S. Y., Fadel, H. H. and Mohamed, D. A. (2015) Phytochemical Constituents, Antioxidant and Anticancer Activity of Mentha citrata and Mentha longifolia. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 6(1): 739-751.
Areias, F. M., Valentao, P., Andrade, P. B., Ferreres, F. and Seabra, R. M. (2001) Phenolic fingerprint of peppermint leaves. Food Chemisry. 73(3): 307-311.
Barchan, A., Bakkali, M., Arakrak, A., Pagán, R. and Laglaoui, A. (2014) The effects of sol‑vents polaritiy on the phenolic contents and antioxidant activity of three Mentha species extracts. International Journal of Current Microbiology and Applied Sciences. 3(11): 399-412.
Bimakr, M., Rahman, R. A., Taip, F. S., Ganjloo, A., Salleh, L. M., Selamat, J. and  Zaidul, I. S. M. (2011) Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and bioproducts processing. 89(1): 67-72.
Biswas, A. K., Chatli, M. K. and Sahoo, J. (2012) Antioxidant potential of curry (Murraya koenigii L.) and mint (Mentha spicata) leaf extracts and their effect on colour and oxidative stability of raw ground pork meat during refrigeration storage. Food chemistry. 133(2): 467-472.
Blois, M. S. (1958)  Antioxidant determinations by the use of a stable free radical. Nature. 1199-1200.
Brahmi, F., Boulkbache-Makhlouf, L., Yalaoui-Guellal, D., Chibane, M. and Madani, K. H. (2014) Comparative study on the antioxidant effect of aqueous and ethanolicextracts of Mentha pulegium L. grown at two different locations. Phyto Chemistry and Bio Sub Journal. 8(3): 138-149.
Chirinos, R., Rogez, H., Campos, D., Pedreschi, R. and Larondelle, Y. (2007) Optimization of extraction conditions of antioxidant phenolic compounds from mashua (TropaeolumtuberosumRuíz and Pavón) tubers. Separation and Purification Technology. 55(2): 217-225.
Damein Dorman, H.J., Kosar, M., Kahlos, K., Holm, Y. and Hiltunen, R. (2003) Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. Journal of Agricultural and Food Chemistry. 51: 4563-4569.
Fatiha, B., Didier, H., Naima, G., Khodir, M., Martin, K., Léocadie, K. and Pierre, D. (2015) Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae). Industrial Crops and Products. 74: 722-730.
Fernández-Ponce, M. T., Casas, L., Mantell, C., Rodríguez, M., and de la Ossa, E. M. (2012) Extraction of antioxidant compounds from different varieties of Mangifera indica leaves using green technologies. The Journal of Supercritical Fluids. 72: 168-175.
Gao, L., and Mazza, G. (1996) Extraction of anthocyanin pigments from purple sunflower hulls. Journal of Food Science. 61(3): 600-603.
Ghaderi, P., Ahmadi, R., Balkanyian, F., Moridikyia, A., Mahdavi, E. and Tavakoli, P. (2014). In-vitro antibacterial activity of Bunium persicum and Mentha longifolia against Bacillus subtilis and Staphylococcus aureus.International Conference on Chemical, Agricultural and Medical Sciences, May 2-3, Antalya, Turkey.
Goli, A. H., Barzegar, M. and Sahari, M. A. (2005) Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chemistry. 92(3): 521-525.
Herzi, N., Bouajila, J., Camy, S., Romdhane, M. and Condoret, J. S. (2013) Comparison of different methods for extraction from Tetraclinis articulata: Yield, chemical composition and antioxidant activity. Food Chemistry.  141 (4): 3537-3545.
Horwitz, W. (1984) Official Methods of Analysis of the Association of Official Analytical Chemists. Association of Official Analytical Chemists. 1-771.
Jayaprakasha, G. K., Girennavar, B. and Patil, B. S. (2008) Radical scavenging activities of Rio Red grapefruits and Sour orange fruit extracts in different in vitro model systems. Bioresource Technology. 99(10): 4484-4494.
Kamkar, A., Jebelli Javan, A., Asadi, F. and Kamalinejad, M. (2010) The antioxidative effect of Iranian Mentha pulegium extracts and essential oil in sunflower oil. Food and Chemical Toxicology. 48: 1796–1800.
Kothari, V. (2011) Antimicrobial and Antioxidant Properties of Plant Products, Lambert Academic Publishing: Germany; 6.
Kothari, V., Gupta, A., and Naraniwal, M. (2012) Comparative study of various methods for extraction of antioxidant and antibacterial compounds from plant seeds. Journal of natural remedies. 12(2): 162-173.
Kulisic, T., Radonic, A., Katalinic, V. and Milos, M. (2004) Use of different methods for testing antioxidative activity of oregano essential oil. Food chemistry. 85(4): 633-640.
Lakić, N. S., Mimica-Dukić, N. M., Isak, J. M. and Božin, B. N. (2010) Antioxidant properties of Galium verum L. (Rubiaceae) extracts. Central European Journal of Biology. 5(3): 331-337.
Luque de Castro, M. D. and Garcia-Ayuso, L. E. (1998) Soxhlet extraction of solid materials: An outdated technique with a promising innovative future. Analytical Chemical Acta. 369: 1-10.
Mata, A. T., Proença, C., Ferreira, A. R., Serralheiro, M. L. M., Nogueira, J. M. F. and Araújo, M. E. M. (2007) Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food chemistry. 103(3): 778-786.
Mathew, S. and Abraham, T.E. (2006) In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology. 44(2): 198-206.
Nickavar, B., Alinaghi, A., Kamalinejad, M. (2008) Evaluation of the antioxidant properties of five Mentha species. Iranian Journal of Pharmaceutical Research. 7(3): 203–209.
Omidbaigi, R. (2005) Production and processing of medicinal plants. Vol. 3. Astane Quds Publ. Tehran, 347 p.
Prieto, P., Pineda, M., and Aguilar, M. (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 269(2): 337-341.
Pinelo, M., Rubilar, M., Jerez, M., Sineiro, J., and Núñez, M. J. (2005) Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. Journal of Agricultural and Food Chemistry. 53(6): 2111-2117.
Proestos, C., and Komaitis, M. (2008) Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds. LWT-food science and technology. 41(4): 652-659.
Riekandeh, S. M., Mazandarani, M., Ebrahimzadeh, M. A., and Zargari, M. (2016) Antioxidant activities of Eryngium caucasicum Inflorescence. European Review for Medical and Pharmacological Sciences. 20(5): 946-949.
Sefidkon, F. (2007) Chemistry and Industrial Preparation of Essential oils. Zavesh, Iran, Tehran, 254 p.
Sefidkon, F., Abbasi, K., Jamzad, Z., Ahmadi, S. (2007). The effect of distillation methods and stage of plant growth on the essential oil content and composition of satureja rechinger jamzad. Journal of Food Chemistry. 100: 1054-1058.
Serra Bonvehí, J., Soliva Torrentó, M. and Centelles Lorente, E. (2001) Evaluation of polyphenolic and flavonoid compounds in honeybee-collected pollen produced in spain. Journal of Agricultural and Food Chemistry. 49(4): 1848-1853.
Stahl-Biskup, E. and Saez, F. (2002)  Thyme the genus thymus. NY, NJ: Taylor and Francis.
Stanisavljević, D., Dordevic, S., Milenkovic, M., Lazic, M., Velickovic, D., Randelovic, N. and Zlatkovic, B. (2014). Antimicrobial and antioxidant activity of the essential oils obtained from Mentha longifolia L. Hudson, dried by three different techniques. Records of Natural products. 8(1): 61.
Suhaj, M. (2006) Spice antioxidants isolation and their antiradical activity: a review. Journal of Food Composition and Analysis. 19(6-7): 531-537.
Sultana, B., Anwar F. and Ashraf M. (2009) Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 14: 2167-2180.
Sweetie, R.K., Chander, R. and Sharma, A. (2007) Antioxidant potential of mint (Mentha spicata L.) in radiation-processedlamb meat. Food Chemistry. 100: 451–458.
Taghiloo, A.H.,) 2010( Evaluation of spicula horsemint population are native to Iran by morphological traits and RAPD markers. Master's thesis of Horticulture sciences, Tehran University, 57 p.
Teixeira, B., Marques, A., Ramos, C., Batista, I., Serrano, C., Matos, O. and Nunes, M. L. (2012) European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products. 36(1): 81-87.
Tuberoso, C. I. G., Rosa, A., Bifulco, E., Melis, M. P., Atzeri, A., Pirisi, F. M. and Dessì, M. A. (2010) Chemical composition and antioxidant activities of Myrtus communis L. berries extracts. Food Chemistry. 123(4): 1242-1251.
Unnithan, C. R., Gebreselassie, H., Sushen, U., Reddy, D. N., Woldu, A. and Muuz, M. (2013) Chemical composition and antibacterial activity of essential oil of Mentha longifolia L of Mekole, Ethiopia. Journal of Biological and Scientific Opinion. 1(3): 151-153.
Yilmaz, Y., and Toledo, R. T. (2004) Major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid. Journal of agricultural and Food Chemistry, 52(2): 255-260.