Isolation and Identification of a Myxobacterium Producing Myxothiazol and Althiomycin from Iran Soil

Moradi, Azam; Yaghoobi Avini, Mohammad; Ebrahimipour, GholamHousein; Ghasempour, AliReza

doi:10.22051/jab.2019.4313

Document Type : Research Paper

Authors

¹ Department of Microbiology and Microbial Biotechnology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University, Tehran, Iran

² Department of Microbiology and Microbial Technology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University, Tehran, Iran

³ Institute of Medicinal Plants and Raw Materials, Shahid Beheshti University, Tehran, Iran

https://doi.org/10.22051/jab.2019.4313

Abstract

Myxobacteria are soil bacteria that move by gliding and have an astonishing life cycle culminating in fruiting body formation. The myxobacterial strain no.118 was isolated from unexplored soil of Koohrang County, Chaharmahal-o-Bakhtiari Province and tested for potential antimicrobial activity against various human pathogens. On the basis of results, strain 118 significantly inhibited growth of E. coli andM. luteus therefore was used for further characterization. Analysis of morphological, biochemical and 16S rRNA gene sequence indicated that this strain belongs to the genus Myxococcus. In addition, neighbor-joining phylogenetic tree confirmed the relationships of this strain to other members of Myxococcus genera. In order to explore the potential bioactivities, extract of the fermented broth culture was prepared with organic solvent extraction method. The methanol extract was subjected to HPLC fractionation against sensitive pathogens. LC/MS analysis resulted in the identification of Myxothiazol and Althiomycinantibiotics in methanol extract of the strain no. 118.

Keywords

Main Subjects

Microbiology

References

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Degli Esposti, Mauro. (1998). Inhibitors of NADH–ubiquinone reductase: an overview. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1364(2), 222-235.

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Eugene Rosenberg, Edward F. DeLong, Stephen Loy, Erko Stackebrandt and Fabiano Thompson (2014). Deltaproteobacteria and Epsilonproteobacteria (4th ed.). Berlin: Springer-verlag.

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Reichenbach, H. (2001). Myxobacteria, producers of novel bioactive substances. Journal of Industrial Microbiology and Biotechnology, 27(3), 149-156.

Reichenbach, H., & Dworkin, M. (1992). The myxobacteria The prokaryotes (pp. 3416-3487): Springer.

Reichenbach, H., & Höfle, G. (1999). Myxobacteria as producers of secondary metabolites. Drug discovery from nature, 149-179.

Reichenbach, H. & Dworkin, M. (2001). The myxobacteria. The Prokaryotes 4.

Shimkets, Lawrence J, Dworkin, Martin, & Reichenbach, Hans. (2006). The myxobacteria The prokaryotes (pp. 31-115): Springer.

Spröer, C., Reichenbach, H., & Stackebrandt, E. (1999). The correlation between morphological and phylogenetic classification of myxobacteria. International Journal of Systematic and Evolutionary Microbiology, 49(3), 1255-1262.

Weissman, K. J., & Müller, R (2010) Myxobacterial secondary metabolites: bioactivities and modes-of-action. Natural product reports, 27(9), 1276-1295.

Wiegand, Irith, Hilpert, Kai, & Hancock, Robert EW. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols, 3(2), 163-175.

Zhang, L., Wang, H., Fang, X., Stackebrandt, E., & Ding, Y. (2003). Improved methods of isolation and purification of myxobacteria and development of fruiting body formation of two strains. Journal of microbiological methods, 54(1), 21-27.

Applied Biology

Isolation and Identification of a Myxobacterium Producing Myxothiazol and Althiomycin from Iran Soil

References

References

Cazin, J., Wiemer, D. F., & Howard, J. J. (1989). Isolation, growth characteristics, and long-term storage of fungi cultivated by attine ants. Applied and environmental microbiology, 55(6), 1346-1350.

Dawid, Wolfgang. (2000). Biology and global distribution of myxobacteria in soils. FEMS microbiology reviews, 24(4), 403-427.

Degli Esposti, Mauro. (1998). Inhibitors of NADH–ubiquinone reductase: an overview. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1364(2), 222-235.

Dworkin, Martin. (1996) Recent advances in the social and developmental biology of the myxobacteria. Microbiological reviews, 60(1), 70.

Eugene Rosenberg, Edward F. DeLong, Stephen Loy, Erko Stackebrandt and Fabiano Thompson (2014). Deltaproteobacteria and Epsilonproteobacteria (4th ed.). Berlin: Springer-verlag.

Fujimoto, H, Kinoshita, Tadatoshi, Suzuki, Hideo, & Umezawa, Hamao. (1970). Studies on the mode of action of althiomycin. The Journal of antibiotics, 23(6), 271-275.

Kunze, B., Reichenbach, H, Augustiniak, H, & Höfle, G. (1982). Isolation and identification of althiomycin from Cystobacter fuscus (Myxobacterales). The Journal of antibiotics, 35(5), 635-636.

Reichenbach, H. (2001). Myxobacteria, producers of novel bioactive substances. Journal of Industrial Microbiology and Biotechnology, 27(3), 149-156.

Reichenbach, H., & Dworkin, M. (1992). The myxobacteria The prokaryotes (pp. 3416-3487): Springer.

Reichenbach, H., & Höfle, G. (1999). Myxobacteria as producers of secondary metabolites. Drug discovery from nature, 149-179.

Reichenbach, H. & Dworkin, M. (2001). The myxobacteria. The Prokaryotes 4.

Shimkets, Lawrence J, Dworkin, Martin, & Reichenbach, Hans. (2006). The myxobacteria The prokaryotes (pp. 31-115): Springer.

Spröer, C., Reichenbach, H., & Stackebrandt, E. (1999). The correlation between morphological and phylogenetic classification of myxobacteria. International Journal of Systematic and Evolutionary Microbiology, 49(3), 1255-1262.

Weissman, K. J., & Müller, R (2010) Myxobacterial secondary metabolites: bioactivities and modes-of-action. Natural product reports, 27(9), 1276-1295.

Wiegand, Irith, Hilpert, Kai, & Hancock, Robert EW. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols, 3(2), 163-175.

Zhang, L., Wang, H., Fang, X., Stackebrandt, E., & Ding, Y. (2003). Improved methods of isolation and purification of myxobacteria and development of fruiting body formation of two strains. Journal of microbiological methods, 54(1), 21-27.

Volume 32, Issue 1 - Serial Number 59
June 2019
Pages 125-137

Isolation and Identification of a Myxobacterium Producing Myxothiazol and Althiomycin from Iran Soil

References

References

Cazin, J., Wiemer, D. F., & Howard, J. J. (1989). Isolation, growth characteristics, and long-term storage of fungi cultivated by attine ants. Applied and environmental microbiology, 55(6), 1346-1350.

Dawid, Wolfgang. (2000). Biology and global distribution of myxobacteria in soils. FEMS microbiology reviews, 24(4), 403-427.

Degli Esposti, Mauro. (1998). Inhibitors of NADH–ubiquinone reductase: an overview. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1364(2), 222-235.

Dworkin, Martin. (1996) Recent advances in the social and developmental biology of the myxobacteria. Microbiological reviews, 60(1), 70.

Eugene Rosenberg, Edward F. DeLong, Stephen Loy, Erko Stackebrandt and Fabiano Thompson (2014). Deltaproteobacteria and Epsilonproteobacteria (4th ed.). Berlin: Springer-verlag.

Fujimoto, H, Kinoshita, Tadatoshi, Suzuki, Hideo, & Umezawa, Hamao. (1970). Studies on the mode of action of althiomycin. The Journal of antibiotics, 23(6), 271-275.

Kunze, B., Reichenbach, H, Augustiniak, H, & Höfle, G. (1982). Isolation and identification of althiomycin from Cystobacter fuscus (Myxobacterales). The Journal of antibiotics, 35(5), 635-636.

Reichenbach, H. (2001). Myxobacteria, producers of novel bioactive substances. Journal of Industrial Microbiology and Biotechnology, 27(3), 149-156.

Reichenbach, H., & Dworkin, M. (1992). The myxobacteria The prokaryotes (pp. 3416-3487): Springer.

Reichenbach, H., & Höfle, G. (1999). Myxobacteria as producers of secondary metabolites. Drug discovery from nature, 149-179.

Reichenbach, H. & Dworkin, M. (2001). The myxobacteria. The Prokaryotes 4.

Shimkets, Lawrence J, Dworkin, Martin, & Reichenbach, Hans. (2006). The myxobacteria The prokaryotes (pp. 31-115): Springer.

Spröer, C., Reichenbach, H., & Stackebrandt, E. (1999). The correlation between morphological and phylogenetic classification of myxobacteria. International Journal of Systematic and Evolutionary Microbiology, 49(3), 1255-1262.

Weissman, K. J., & Müller, R (2010) Myxobacterial secondary metabolites: bioactivities and modes-of-action. Natural product reports, 27(9), 1276-1295.

Wiegand, Irith, Hilpert, Kai, & Hancock, Robert EW. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols, 3(2), 163-175.

Zhang, L., Wang, H., Fang, X., Stackebrandt, E., & Ding, Y. (2003). Improved methods of isolation and purification of myxobacteria and development of fruiting body formation of two strains. Journal of microbiological methods, 54(1), 21-27.

Volume 32, Issue 1 - Serial Number 59June 2019Pages 125-137

Volume 32, Issue 1 - Serial Number 59
June 2019
Pages 125-137