Document Type : Research Paper

Authors

1 Associate Professor of Biotechnology department, Faculty of biological Sciences, Alzahra University, Tehran

2 M.Sc in microbiology, Faculty of biological Sciences, Alzahra University, Tehran

3 Assistant Professor of Biotechnology department, Faculty of biological Sciences, Alzahra University, Tehran

Abstract

Catalase is an enzyme capable of catalyzing the alteration of H2O2 to O2 and H2O. It has recently acquired importance due to its application in the textile industries. Kocuria ASB107shows relative high resistance against ionizing and UV radiation. the antioxidant barrier in this bacterium consists enzymes such as catalase. In order to achieve the highest rate of bacterial growth and catalase production, sugar cane molasses, sugar beet molasses and whey were used as cheap carbon sources. Growth curves were plotted and at the late logarithmic phase, fermentation product was harvested. Catalase activity was measured spectrophotometrically by monitoring the decrease in absorbance at 240 nm affected by the decomposition of hydrogen peroxide. bacterial growth was also stimated from the weight of dry biomass. The highest biomass (5.19 g/L) and catalase activity (2136.25 U/mL) were found in medium consisted 1% molasses and 2.5 % yeast extract. In addition with the 4% whey as a carbon source, catalase activity and growth were 3032.5 U/mL and 6.16 g/L respectively. The results showed molasses and whey are suitable and inexpensive substrate. on the other hand inorganic nitrogen sources such as urea are not suitable for the production of catalase and cell growth.

Keywords

Main Subjects

Amorim, A.M., Gasques, M.G., Andreaus, J. and Scharf, M. (2002).  The application of catalase for the elimination of hydrogen peroxide residues after bleaching of cotton fabric. Annals of the Brazilian Academy of Sciences, 74: 433-436.
Aruldoss, V. and Kalaichelvan, P.T. (2014).  Production of catalase by solid state fermentation using different agro and fruit peel wastes as substrates. Journal of Modern Biotechnology, 3: 8 – 13.
Asgarani, E., Soudi, M.R., Borzooee, F. and Dabbagh, R. (2012).  Radio- resistance in psychrotrophic Kocuri asp. ASB 107 isolated from Ab-e-Siah radioactive spring. Journal of Environmental Radioactivity, 113: 171-176.
Capua, CD., Bortolotti, A., Far´ıas, M. and Cortez, N. (2011). UV-resistant Acinetobacter sp. isolates from Andeanwet lands display high catalase activity. FEMS Microbiology Letters, 317: 181-189.
Dehghan, M., Moosavi-Nejad, Z., Gharavi, S. and Fooladi, J. (2013).  Cane molasses as asource of precursors in the bioproduction of tryptophan by Bacillus subtilis. Irainian Journal of Microbiology, 5: 285-292.
Hua, Z., Yan, G., Du, G. and Chen, J. (2007). Study and improvement of the conditions for production of a novel alkali stable catalase. Biotechnology Journal, 2: 326–333.
Rochat, T., Gratadoux, J., Gruss, A., Corthier, G., Maguin, E., Langella, P. and Guchte, M. (2006).  Production of a heterologous nonheme catalase by Lactobacillus casei: an efficient tool for removal of H2O2 and protection of Lactobacillus bulgaricusfrom oxidative stress in milk. Applied and Environmental Microbiology, 72:5143.
Savvides, A.L., Katsifas, E.A., Hatzinikolaou, D., Karagouni, D.A. (2012). Xanthan production by Xanthomonas campestris using whey permeate medium. World Journal of Microbiology and Biotechnology, 28:2759–2764.
Schultz, D. and Kishony, R. (2013). Optimization and control in bacterial Lag phase. BMC Biology, 11: 120.
Shi, X., Feng, M., Zhao, Y., Guo, X. and Zhou, P. (2008). Overexpression, purification and characterization of a recombinant secretary catalase from Bacillus subtilis. Biotechnology Letters, 30:181–186.
Shikha, R., Sharan, A. and Darmwal, N. (2007). Improved production of alkaline protease from a mutant of alkalophilic Bacillus pantotheneticus using molasses as a substrate. Bioresource Technology, 98: 881-885.
Silva, M., Fornari, R.C. and Mazutti, M.A. (2009). Production and characterization of xantham gum by Xanthomonas campestris using cheese whey as sole carbon source. Journal of Food and Engineering, 90: 119-123.
Soung, N.K. and Lee, Y.N. (2000). Iso-catalase Profiles of Deinococcus spp.  Journal of Biochemistry and Molecular Biology, 33: 412- 416.
Xiao, Z.J., Liu, J.Y. and Qin, P. (2007). Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate. Applied Microbiology and Biotechnology, 74: 61-68.
Zeng, H.W., Cai, Y.J., Liao, X.R., Qian, S.L., Zhang, F. and Zhang, D.B. (2010). Optimization of catalase production and purification and characterization of a novel cold-adapted Cat-2 from mesophilic bacterium Serratia marcescens SYBC-01. Annals of Microbiology, 60:701–708.
Zeng, H.W., Cai, Y.J., Liao, X.R., Zhang, F., Li, Y.L., Zeng, X.K. and Zhang, D.B. (2011). Serratia marcescens SYBC08 catalase isolated from sludge containing hydrogen peroxide shows increased catalase production by regulation of carbon metabolism. Engineering in Life Sciences, 11: 37–43.