Document Type : Research Paper

Authors

• Paricher Hanachi * ¹
• zahra Ghaseminya ²
• Khsroo Sadeghnyat Hagigi ³
• Abolfazl Golestani ⁴

¹ .Biotechnology Dep, Faculty of Biological Sciences, Alzahra University,Tehran Iran

² Occupational Medicine Dep , Tehran Medical University, Tehran Iran

³ Associate professor, Department of plant biology, Islamic Azad University, Gorgan branch, Gorgan, Golestan

⁴ Associate Professor, Department of Plant Biology, Islamic Azad University, Gorgan branch, Gorgan, Golestan.

Abstract

The aim of this study was to evaluate possible effect on antioxidant status on   ghost protein carbonyl and protein sulfhydryl content and ghost and plasma.
Methods: The 35 subjects referred to sleep laboratory were examined by polysomnography for obstructive sleep apnea. According to AHI (Apnea-hypopnea index), patients were categorized in two groups; mild (n=17) and severe (n=18). The fasting blood samples taken in morning after sleep study for protein carbonyl, protein sulfhydryl, and Malondialdehyde (MDA) assays as antioxidant indexes and confounding factors such as smoking and high blood pressure were studied.
Results: The mean of protein carbonyl were 4.17 ± 0.42 nmol/mg and 5.59 ± 0/49 nmol/mg and protein sulfhydryl content 7.09 ± 0.66 and 8.70 ± 2.03 in patients with mild and severe obstructive sleep apnea respectively. For MDA content in red blood cells 0.25 ± 0.02 nmol/mg, 0.28 ± 0.04 nmol/mg, and MDA content of plasma were 0.93 ± 0.06 nmol/mL and 1.35 ± 0.29 nmol/mL in mild and sever OSA patient for respectively. Protein carbonyl content was known as the only biomarker that showed significant (p<0.05) difference in both groups. .
Conclusion: According to the results in this study it can be proposed that protein carbonyl content in patient with sever obstructive sleep apnea has changed significantly. It may be a consequence of oxidative stress effect on membrane protein resulted from apnea.

Keywords

• Free radicals
• Obstructive sleep apnea
• Oxidative stress